Cytostatic drug sensitivity test for human multiple myeloma, measuring monoclonal immunoglobulin produced by bone marrow cells in vitro

1986 ◽  
Vol 17 (1) ◽  
pp. 69-74 ◽  
Author(s):  
D. Peest ◽  
B. Bartels ◽  
I. Dallmann ◽  
I. Schedel ◽  
H. Deicher
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Drug Sensitivity ◽  
Bone Marrow Cells ◽  
Sensitivity Test ◽  
Cytostatic Drug ◽  
Monoclonal Immunoglobulin ◽  
Drug Sensitivity Test ◽  
Human Multiple Myeloma

O-45 Drug sensitivity test (in vitro growth assay) of gefitinib (Iressa, ZD1839) for primary lung cancer

Lung Cancer ◽  
2003 ◽  
Vol 41 ◽  
pp. S16
Author(s):  
Motoshi Takao ◽  
Kentaro Inoue ◽  
Fumiaki Watanabe ◽  
Tomohito Tarukawa ◽  
Masanori Kaneda ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Drug Sensitivity ◽  
Primary Lung Cancer ◽  
Sensitivity Test ◽  
In Vitro Growth ◽  
Drug Sensitivity Test ◽  
Growth Assay

Study on the effect of superparamagnetic γ-Fe2O3 combined with carmustine on cervical cancer

2020 ◽  
Vol 10 (8) ◽  
pp. 1213-1217
Author(s):  
Jinghua Xiao ◽  
Yani Li ◽  
Yanxia Wang ◽  
Xinru Wang ◽  
Wanwan Zhang ◽  
...  
Keyword(s):  
Cervical Carcinoma ◽  
Drug Sensitivity ◽  
Combined Treatment ◽  
Scientific Basis ◽  
Carcinoma Cells ◽  
Sensitivity Test ◽  
Control Group ◽  
Multidrug Resistance Gene ◽  
Drug Sensitivity Test

This study aims to investigate the killing effect of γ-Fe2O3 combined with carmustine on cervical carcinoma cells, as well as the mechanism of drug resistance of this combined treatment. RT-PCR was used to detect the expression of Multidrug Resistance Gene (MDR-l) gene in all the samples. Results showed that the combination of γ-Fe2O3 and carmustine drugs was significantly better than the control group (P < 0.05), and the tumor showed different degrees of tolerance in combination with the drug. The results of an in vitro drug sensitivity test were consistent with those of mRNA expression of MDR-l gene (P < 0.04). The combination of superparamagnetic γ-Fe2O3 and carmustine derivatives has a better therapeutic effect on cervical carcinoma than either treatment alone. The drug sensitivity test and MDR-l mRNA detection in vitro can improve the accuracy and predictability of cervical carcinoma chemotherapy, providing a scientific basis for individual tumor chemotherapy.

scholarly journals Production of cytokines by bone marrow cells obtained from patients with multiple myeloma

Blood ◽  
1989 ◽  
Vol 74 (4) ◽  
pp. 1266-1273 ◽  
Author(s):  
A Lichtenstein ◽  
J Berenson ◽  
D Norman ◽  
MP Chang ◽  
A Carlile
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Interleukin 1 ◽  
Interleukin 2 ◽  
In Vitro Production ◽  
Osteolytic Bone Disease ◽  
Vitro Production ◽  
Necrosis Factor

Abstract Previous work with continuously cultured multiple myeloma lines suggested that cytokine production by tumor cells may mediate some of the medical complications of this disease. To further investigate this issue, we assayed freshly obtained bone marrow (BM) cells from myeloma patients for the in vitro production of cytokines and the presence of cytokine RNA. Production of cytokine protein was assessed by bioassays with the aid of specific neutralizing anticytokine antibodies. These assays detected interleukin-1 (IL-1) and tumor necrosis factor (TNF) secretion by myeloma BM cells, which was significantly greater than secretion from similarly processed BM cells of control individuals. In contrast, lymphotoxin and interleukin-2 (IL-2) production could not be detected. The levels of IL-1 and TNF produced in vitro peaked at 24 hours of culture and correlated with stage and the presence (or absence) of extensive osteolytic bone disease. Northern blot analysis demonstrated the presence of IL-1 beta and TNF RNA in uncultured myeloma BM cells but no detectable IL-1 alpha or lymphotoxin RNA. In addition, the amount of cytokine RNA correlated with protein production, being significantly greater in patients' BM cells than in control marrow. These data suggest a role for IL-1 beta and/or TNF in the pathophysiology of multiple myeloma and argue against a role for lymphotoxin or IL-2.

In vitro-in vivo Correlation in Anticancer Drug Sensitivity Test Using AUC-Based Concentrations and Collagen Gel Droplet-Embedded Culture

Oncology ◽  
1996 ◽  
Vol 53 (3) ◽  
pp. 250-257 ◽  
Author(s):  
Makoto Inaba ◽  
Tazuko Tashiro ◽  
Shigeo Sato ◽  
Yasuyuki Ohnishi ◽  
Keizo Tanisaka ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Drug Sensitivity ◽  
Collagen Gel ◽  
Sensitivity Test ◽  
Drug Sensitivity Test

Verification of biomarkers in predicting drug sensitivity with in vitro 3D drug sensitivity test in non-small cell lung cancer.

2016 ◽  
Vol 34 (15_suppl) ◽  
pp. 11602-11602
Author(s):  
Dage Liu ◽  
Nariyasu Nakashima ◽  
Yoshuke Kita ◽  
Yoshimasa Tokunaga ◽  
Kazuhito Nii ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Drug Sensitivity ◽  
Small Cell ◽  
Sensitivity Test ◽  
Small Cell Lung ◽  
Drug Sensitivity Test

3200 Effect of chemotherapy drugs in vitro on the death of bone marrow cells in patients with multiple myeloma

2015 ◽  
Vol 51 ◽  
pp. S649
Author(s):  
I. Lysenko ◽  
E. Zlatnik ◽  
I. Novikova ◽  
N. Nikolaeva ◽  
O. Shatokhina ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Chemotherapy Drugs ◽  
Marrow Cells
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