Comparison of an endogenous protein kinase C substrate in rat aorta with rat brain MARCKS

1992 ◽  
Vol 116 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Dayuan Zhao ◽  
Morley D. Hollenberg ◽  
David L. Severson
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Rat Aorta ◽  
Kinase C ◽  
Endogenous Protein

scholarly journals Two components of type III protein kinase C with different substrate specificities and a phospholipid-dependent but Ca2+ -inhibited protein kinase in rat brain

FEBS Letters ◽  
1989 ◽  
Vol 249 (2) ◽  
pp. 324-328 ◽  
Author(s):  
Lázló Buday ◽  
György Mészáros ◽  
Gyöngyi Farkas ◽  
János Seprődi ◽  
Ferenc Antoni ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Substrate Specificities ◽  
Type Iii ◽  
Kinase C

scholarly journals Mammalian Homologue of the Caenorhabditis elegans UNC-76 Protein Involved in Axonal Outgrowth Is a Protein Kinase C ζ–interacting Protein

1999 ◽  
Vol 144 (3) ◽  
pp. 403-411 ◽  
Author(s):  
Shun'ichi Kuroda ◽  
Noritaka Nakagawa ◽  
Chiharu Tokunaga ◽  
Kenji Tatematsu ◽  
Katsuyuki Tanizawa
Keyword(s):  
Plasma Membrane ◽  
Protein Kinase C ◽  
Caenorhabditis Elegans ◽  
Protein Kinase ◽  
Rat Brain ◽  
Axonal Outgrowth ◽  
Yeast Two Hybrid ◽  
Interacting Protein ◽  
Kinase C ◽  
Two Hybrid

By the yeast two-hybrid screening of a rat brain cDNA library with the regulatory domain of protein kinase C ζ (PKCζ) as a bait, we have cloned a gene coding for a novel PKCζ-interacting protein homologous to the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth and fasciculation. The protein designated FEZ1 (fasciculation and elongation protein zeta-1) consisting of 393 amino acid residues shows a high Asp/Glu content and contains several regions predicted to form amphipathic helices. Northern blot analysis has revealed that FEZ1 mRNA is abundantly expressed in adult rat brain and throughout the developmental stages of mouse embryo. By the yeast two-hybrid assay with various deletion mutants of PKC, FEZ1 was shown to interact with the NH2-terminal variable region (V1) of PKCζ and weakly with that of PKCε. In the COS-7 cells coexpressing FEZ1 and PKCζ, FEZ1 was present mainly in the plasma membrane, associating with PKCζ and being phosphorylated. These results indicate that FEZ1 is a novel substrate of PKCζ. When the constitutively active mutant of PKCζ was used, FEZ1 was found in the cytoplasm of COS-7 cells. Upon treatment of the cells with a PKC inhibitor, staurosporin, FEZ1 was translocated from the cytoplasm to the plasma membrane, suggesting that the cytoplasmic translocation of FEZ1 is directly regulated by the PKCζ activity. Although expression of FEZ1 alone had no effect on PC12 cells, coexpression of FEZ1 and constitutively active PKCζ stimulated the neuronal differentiation of PC12 cells. Combined with the recent finding that a human FEZ1 protein is able to complement the function of UNC-76 necessary for normal axonal bundling and elongation within axon bundles in the nematode, these results suggest that FEZ1 plays a crucial role in the axon guidance machinery in mammals by interacting with PKCζ.

Characterization of Phorbol Ester Binding to Membrane-Inserted Protein Kinase C in Rat Brain Membrane

1990 ◽  
Vol 44 (1) ◽  
pp. 141-142
Author(s):  
Ken'ichi Osada ◽  
Mikio Asakura ◽  
Makiko Shibata ◽  
Tohru Tsukamoto ◽  
Jun Imafuku ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Phorbol Ester ◽  
Brain Membrane ◽  
Kinase C

Protein kinase C-delta in rat brain: Association with sensory neuronal hierarchies

1993 ◽  
Vol 331 (3) ◽  
pp. 375-388 ◽  
Author(s):  
Meredith M. Garcia ◽  
Catherine G. Cusick ◽  
Richard E. Harlan
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Protein Kinase C Delta ◽  
Kinase C
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