2-Heptanone and 10-hydroxy-trans-dec-2-enoic acid in the mandibular glands of worker honey bees of different ages

1966 ◽  
Vol 54 (1) ◽  
pp. 1-11 ◽  
Author(s):  
R. Boch ◽  
D. A. Shearer
Keyword(s):  
Honey Bees ◽  
Enoic Acid ◽  
Mandibular Glands ◽  
Different Ages

scholarly journals Deformed Wing Virus Infection in Honey Bees (Apis mellifera L.)

2019 ◽  
Vol 56 (4) ◽  
pp. 636-641 ◽  
Author(s):  
Roman V. Koziy ◽  
Sarah C. Wood ◽  
Ivanna V. Kozii ◽  
Claire Janse van Rensburg ◽  
Igor Moshynskyy ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Rna Virus ◽  
Mandibular Glands ◽  
Deformed Wing Virus ◽  
Clinically Normal ◽  
Bee Health ◽  
Honey Bee Health ◽  
Honey Bee Workers

Deformed wing virus (DWV) is a single-stranded RNA virus of honey bees ( Apis mellifera L.) transmitted by the parasitic mite Varroa destructor. Although DWV represents a major threat to honey bee health worldwide, the pathological basis of DWV infection is not well documented. The objective of this study was to investigate clinicopathological and histological aspects of natural DWV infection in honey bee workers. Emergence of worker honey bees was observed in 5 colonies that were clinically affected with DWV and the newly emerged bees were collected for histopathology. DWV-affected bees were 2 times slower to emerge and had 30% higher mortality compared to clinically normal bees. Hypopharyngeal glands in bees with DWV were hypoplastic, with fewer intracytoplasmic secretory vesicles; cells affected by apoptosis were observed more frequently. Mandibular glands were hypoplastic and were lined by cuboidal epithelium in severely affected bees compared to tall columnar epithelium in nonaffected bees. The DWV load was on average 1.7 × 106 times higher ( P < .001) in the severely affected workers compared to aged-matched sister honey bee workers that were not affected by deformed wing disease based on gross examination. Thus, DWV infection is associated with prolonged emergence, increased mortality during emergence, and hypoplasia of hypopharyngeal and mandibular glands in newly emerged worker honey bees in addition to previously reported deformed wing abnormalities.

scholarly journals Effects of the Age of Grafted Larvae and the Effects of Supplemental Feeding on Some Morphological Characteristics of Iranian Queen Honey Bees (Apis mellifera meda Skorikov, 1929)

2012 ◽  
Vol 56 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Ali Mahbobi ◽  
Mohammadbagher Farshineh-Adl ◽  
Jerzy Woyke ◽  
Saeed Abbasi
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Wing Length ◽  
Age Groups ◽  
Morphological Characteristics ◽  
Supplemental Feeding ◽  
Feeding Groups ◽  
Different Ages ◽  
Apis Mellifera Meda

Effects of the Age of Grafted Larvae and the Effects of Supplemental Feeding on Some Morphological Characteristics of Iranian Queen Honey Bees (Apis mellifera medaSkorikov, 1929)The research was conducted at the apiary of the Faculty of Agriculture, Zanjan University in Zanjan, Iran. Queens were reared in 24Apis mellifera medahoney bee rearing colonies. The colonies were assigned to 4 grafting larvae age groups; 1 day old larvae, 2 day old larvae, and 3 day old larvae, and the last group reared emergency queen cells. The groups were divided into the 2 feeding groups: fed additionally and no fed. The effects of the age of the grafted larvae and the effects of supplemental feeding on 9 morphological characteristics of queens were measured. The results showed that the age of the larvae significantly affected the morphological characteristics of reared queens, and thus, their quality. Queens reared from 1 day old larvae were of the highest quality. These queens were significantly heavier (158.83 mg) and had significantly larger spermatheca (0.99 mm3) than queens reared from larvae 2 and 3 days old. Queens from emergency queen cells were of lower quality than queens reared from 1 day old larvae. However, queens from emergency queen cells were of higher quality than queens reared from 3 day old larvae. The supplemental feeding significantly increased most morphological characteristics of the reared queens. The different ages of the larvae did not significantly affect the wing length nor did supplemental feeding affect the wing length.

Phospholipase A2 in venom extracts from honey bees (Apis mellifera L.) of different ages

Toxicon ◽  
1990 ◽  
Vol 28 (7) ◽  
pp. 813-820 ◽  
Author(s):  
Michael D. Owen ◽  
Laurie Ann Pfaff ◽  
Robert E. Reisman ◽  
J. Wypych
Keyword(s):  
Apis Mellifera ◽  
Phospholipase A2 ◽  
Honey Bees ◽  
Different Ages

LARVAL REARING BY WORKER HONEY BEES LACKING THEIR MANDIBULAR GLANDS: II. REARING BY LARGER NUMBERS OF WORKER BEES

1979 ◽  
Vol 111 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Ying-Shin Peng ◽  
S. C. Jay
Keyword(s):  
Honey Bees ◽  
Royal Jelly ◽  
Mandibular Gland ◽  
Caste Differentiation ◽  
Larval Rearing ◽  
Mandibular Glands ◽  
Larval Food ◽  
Mandibular Gland Secretion ◽  
Final Development ◽  
Queen Cell

AbstractFurther experiments were done in an attempt to ascertain the significance of the mandibular glands of nurse bees in female caste differentiation. Groups of 200, 10-day-old nurse bees, with their mandibular glands removed, fed female larvae for 80 h in plastic queen cell cups in the laboratory. After this, each larva finished feeding in a 4-day-old queen cell containing "royal jelly"; final development occurred in an artificial pupation dish. Because four adults, classified as "queenlike intermediates," were reared it appears that (1) mandibular gland secretion is less important as a larval food than that of the hypopharyngeal glands, and (2) if a "queen determining substance" exists the mandibular glands are not its only source.

scholarly journals Manganese metabolism in the body of the honey bee (Apis mellifera L.) at different periods of ontogenesis

2020 ◽  
Vol 22 (93) ◽  
pp. 74-78
Author(s):  
Y. Kovalskyi ◽  
L. Kovalska ◽  
P. Golovach ◽  
V. V. Fedak ◽  
A. I. Druzhbiak
Keyword(s):  
Honey Bees ◽  
Biological Material ◽  
Royal Jelly ◽  
Manganese Content ◽  
The Body ◽  
Bee Pollen ◽  
Different Ages ◽  
National University ◽  
A Cell ◽  
One Year

The results of studies of manganese metabolism in honey bees are presented. The study was conducted in conditions of the department of technology of production and processing of small animals of Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lvivy. In the course of work the material selected on an educational apiary of university was investigated and in some apiaries of Lviv region. In particular, samples of different species were taken for study pollen. The study was pollen, which was selected after 15 days of storage in the hive. Along with this, the material for the study was honey, royal jelly, wax. From the biological material we studied the mineral composition of larvae and bees stages of adults of different ages and genders. One-day-old worker bees and drones in May, August, and December were selected to determine seasonal change and sex differences. Research of age changes in larvae 3 and 6 days of age were carried out by sampling biological material from 5 bee families of equal strength and feeding regime. A frame was used to obtain a one-year-old brood - an insulator in which a cell with a uterus for laying eggs was placed. After the bees come out of the cells they were placed in one family. The main sources of its entry into the body have been studied. It is established that the largest amount of manganese is contained in samples of dandelion and winter oilseed rape and is 4.5 mg/100 g of pollen. The smallest amount of Manganese 1.87 mg/100 was recorded in clover skin g. The insignificant content of Manganese in bee pollen from buckwheat of 2.32 mg/100 g. Manganese metabolism in the body of honey bees at different stages of ontogenesis has been studied. In particular, the chemical composition of muscle fibers of adult bees, larvae of different ages was studied. the content of this element in different departments of insects. The dynamics of manganese accumulation is observed not only depending on sex, age, season of the year, and also on physiological features of bee families. The content of Manganese in the main products of beekeeping: royal jelly, honey, wax and bee pollen. According to research, the main number Manganese in the body of honey bees is contained in the cuticle – the outer skeleton that covers it body and chitinous formations that make up the internal skeleton. Its amount averages 1.78 mg/100 g. In the body of the bee, the manganese content varies widely.

Protein feed stimulates the development of mandibular glands of honey bees (Apis mellifera)

2020 ◽  
Vol 60 (1) ◽  
pp. 165-171
Author(s):  
Marcelo P Camilli ◽  
Daniel C B de Barros ◽  
Luis A Justulin ◽  
Marcos L P Tse ◽  
Ricardo de Oliveira Orsi
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Mandibular Glands ◽  
Protein Feed

LARVAL REARING BY WORKER HONEY BEES LACKING THEIR MANDIBULAR GLANDS: I. REARING BY SMALL NUMBERS OF WORKER BEES

1977 ◽  
Vol 109 (9) ◽  
pp. 1175-1180 ◽  
Author(s):  
Ying-Shin Peng ◽  
S. C. Jay
Keyword(s):  
Honey Bees ◽  
Caste Differentiation ◽  
Larval Rearing ◽  
Mandibular Glands ◽  
Queen Cell

AbstractExperiments were done in an attempt to ascertain the significance of the mandibular glands of nurse bees in female caste differentiation. Groups of 30 or 75, 10-day-old nurse bees, with their mandibular glands removed, were caged in the laboratory. Each group was provided with female larvae in plastic queen cell cups. The treated nurse bees were able to rear a few pupae, from these larvae, which were classified as queenlike intermediates while the untreated nurse bees reared pupae or adults which were classified as queens or queenlike intermediates.

The isolation and synthesis of queen substance, 9-oxodec- trans -2-enoic acid, a honeybee pheromone

1962 ◽  
Vol 155 (960) ◽  
pp. 417-432 ◽  
Keyword(s):  
Biological Activity ◽  
Methyl Ester ◽  
Small Mammals ◽  
The Other ◽  
Enoic Acid ◽  
Synthetic Material ◽  
Mandibular Glands ◽  
Queen Rearing ◽  
Wide Range ◽  
Queen Substance

A method is described for isolating ‘queen substance’, which is produced in the mandibular glands of queen honeybees and inhibits queen rearing by worker honeybees and development of their ovaries. Queen substance has been identified as 9-oxodec- trans -2-enoic acid and its identity confirmed by synthesis. The synthetic and natural materials have the same biological activity. The methyl ester of 9-oxodecenoic acid is also active. Neither the natural nor the synthetic material completely inhibits queen rearing, whereas the presence of a live queen does. The other inhibitory factor is ‘queen scent’, which also on its own does not completely inhibit queen rearing. Queen scent plus 9-oxodecenoic acid cause complete inhibition. 9-Oxodecenoic acid did not affect the reproduction of any of the small mammals on which it was tested; nor did it have any pharmacological activity in a wide range of tests.

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