Chromosomal arrangement of heat shock locus 2-48B in Drosophila hydei

Chromosoma ◽  
1982 ◽  
Vol 85 (2) ◽  
pp. 237-249 ◽  
Author(s):  
F. P. A. M. N. Peters ◽  
C. J. Grond ◽  
P. J. A. Sondermeijer ◽  
N. H. Lubsen
Keyword(s):  
Heat Shock ◽  
Drosophila Hydei ◽  
Chromosomal Arrangement ◽  
Shock Locus

The unusual structure of heat shock locus 2-48B in Drosophila hydei

1984 ◽  
Vol 197 (3) ◽  
pp. 392-398 ◽  
Author(s):  
Frans P. A. M. N. Peters ◽  
Nicolette H. Lubsen ◽  
Uwe Walldorf ◽  
Rob J. M. Moormann ◽  
Bernd Hovemann
Keyword(s):  
Heat Shock ◽  
Unusual Structure ◽  
Drosophila Hydei ◽  
Shock Locus

Regulated expression of a Drosophila melanogaster heat shock locus after stable integration in a Drosophila hydei cell line

1985 ◽  
Vol 5 (11) ◽  
pp. 3208-3213
Author(s):  
J H Sinclair ◽  
S E Saunders ◽  
J F Burke ◽  
J H Sang
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cell Line ◽  
Stable Integration ◽  
Drosophila Hydei ◽  
Regulated Expression ◽  
Shock Locus

DNA-mediated cotransformation has been used to transfer a Drosophila melanogaster heat shock locus into cultured Drosophila hydei cells by use of the copia-based selectable vector pCV2gpt and of pMH10A, a cloned 87A7 heat shock locus encoding a mutant heat shock protein (hsp). Transformed lines contain between 50 and 200 copies of both plasmids, each separately organized as a head-to-tail concatemer which is stably maintained in the transformed lines. Exposure of the cotransformants to heat shock temperatures induces the regulated expression of the hsp RNA and the mutant hsp in all the lines analyzed.

scholarly journals Regulated expression of a Drosophila melanogaster heat shock locus after stable integration in a Drosophila hydei cell line.

1985 ◽  
Vol 5 (11) ◽  
pp. 3208-3213 ◽  
Author(s):  
J H Sinclair ◽  
S E Saunders ◽  
J F Burke ◽  
J H Sang
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cell Line ◽  
Stable Integration ◽  
Drosophila Hydei ◽  
Regulated Expression ◽  
Shock Locus

DNA-mediated cotransformation has been used to transfer a Drosophila melanogaster heat shock locus into cultured Drosophila hydei cells by use of the copia-based selectable vector pCV2gpt and of pMH10A, a cloned 87A7 heat shock locus encoding a mutant heat shock protein (hsp). Transformed lines contain between 50 and 200 copies of both plasmids, each separately organized as a head-to-tail concatemer which is stably maintained in the transformed lines. Exposure of the cotransformants to heat shock temperatures induces the regulated expression of the hsp RNA and the mutant hsp in all the lines analyzed.

scholarly journals MUTATIONAL ANALYSIS OF THE REGION SURROUNDING THE 93D HEAT SHOCK LOCUS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1984 ◽  
Vol 106 (2) ◽  
pp. 249-265
Author(s):  
Jym Mohler ◽  
Mary Lou Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Mutational Analysis ◽  
Point Mutations ◽  
Γ Irradiation ◽  
Lethal Mutations ◽  
Complementation Groups ◽  
In Trans ◽  
Shock Locus ◽  
Shock Proteins

ABSTRACT The region containing subdivisions 93C, 93D and 93E on chromosome 3 of Drosophila melanogaster has been screened for visible and lethal mutations. Treatment with three mutagens, γ irradiation, ethyl methanesulfonate and diepoxybutane, has produced mutations that fall into 20 complementation groups, including the previously identified ebony locus. No point mutations affecting the heat shock locus in 93D were detected; however, a pair of deficiencies that overlap in the region of this locus was isolated. Flies heterozygous in trans for this pair of deficiencies are capable of producing all of the major heat shock puffs (except 93D) and the major heat shock proteins. In addition, these flies show recovery of normal protein synthesis following a heat shock.

Deficiency mapping of the 93D heat-shock locus in Drosophila melanogaster

Chromosoma ◽  
1982 ◽  
Vol 86 (4) ◽  
pp. 457-467 ◽  
Author(s):  
Jym Mohler ◽  
Mary Lou Pardue
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Shock Locus

scholarly journals The dynamics of chromatin condensation: redistribution of topoisomerase II in the 87A7 heat shock locus during induction and recovery.

1993 ◽  
Vol 13 (12) ◽  
pp. 7522-7530 ◽  
Author(s):  
A Udvardy ◽  
P Schedl
Keyword(s):  
Heat Shock ◽  
Chromatin Structure ◽  
Topoisomerase Ii ◽  
Chromatin Condensation ◽  
Intergenic Spacer ◽  
Domain Boundaries ◽  
Shock Locus ◽  
Sites Of Action ◽  
Putative Domain

We have examined the in vivo sites of action for topoisomerases II in the 87A7 heat shock locus as a function of gene activity. When the hsp70 genes are induced, there is a dramatic redistribution of topoisomerase II in the locus which parallels many of the observed alterations in chromatin structure. In addition to changes in the topoisomerase II distribution within the locus, we find topoisomerase II localized around the putative domain boundaries scs and scs'. During recovery, when the chromatin fiber of the locus recondenses, the major sites of action for topoisomerase II appear to be located within the two hsp70 genes and in the intergenic spacer separating the two genes.

Genomic organization and functional analysis of a deletion variant of the 87A7 heat shock locus of Drosophila melanogaster

1982 ◽  
Vol 155 (3) ◽  
pp. 267-280 ◽  
Author(s):  
Andor Udvardy ◽  
János Sümegi ◽  
Éva Csordás Tóth ◽  
János Gausz ◽  
Henrik Gyurkovics ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Genomic Organization ◽  
Deletion Variant ◽  
Shock Locus
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