Determination of the calcium binding capacity to discriminate between urines of calcium stone formers and healthy persons

1992 ◽  
Vol 20 (1) ◽  
pp. 101-102
Author(s):  
H. F�redi-Milhofer ◽  
K. Kiss ◽  
F. Kahana ◽  
S. Sarig
Keyword(s):  
Calcium Binding ◽  
Binding Capacity ◽  
Calcium Stone ◽  
Stone Formers ◽  
Healthy Persons

Preparation of dextran–casein phosphopeptide conjugates, evaluation of its calcium binding capacity and digestion in vitro

2021 ◽  
pp. 129332
Author(s):  
Lan Jiang ◽  
Shuhong Li ◽  
Nan Wang ◽  
Shuang Zhao ◽  
Yue Chen ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Binding Capacity ◽  
Casein Phosphopeptide

Changes in the secondary structures and zeta potential of soybean peptide and its calcium complexes in cell membrane medium

2021 ◽  
Author(s):  
He Liu ◽  
Ying Lv ◽  
Jingting Xu ◽  
Chen Chen ◽  
Shuntang Guo
Keyword(s):  
Enzymatic Hydrolysis ◽  
Cell Membrane ◽  
Zeta Potential ◽  
Calcium Binding ◽  
Binding Capacity ◽  
Secondary Structures ◽  
High Calcium ◽  
Calcium Complexes

In this study, soybean peptides (10-30kDa) with high calcium binding capacity were prepared by enzymatic hydrolysis and ultrafiltration. The results of cell experiments showed that the peptide could transport calcium...

Muscle Na+-K+-ATPase response during 16 h of heavy intermittent cycle exercise

2007 ◽  
Vol 293 (2) ◽  
pp. E523-E530 ◽  
Author(s):  
H. J. Green ◽  
T. A. Duhamel ◽  
G. P. Holloway ◽  
J. W. Moule ◽  
J. Ouyang ◽  
...  
Keyword(s):  
Aerobic Power ◽  
Intermittent Exercise ◽  
Binding Capacity ◽  
Vastus Lateralis ◽  
Intrinsic Activity ◽  
Cycle Exercise ◽  
Maximum Binding Capacity ◽  
Phosphatase Assay ◽  
Quantitative Immunoblotting

This study investigated the effects of a 16-h protocol of heavy intermittent exercise on the intrinsic activity and protein and isoform content of skeletal muscle Na+-K+-ATPase. The protocol consisted of 6 min of exercise performed once per hour at ∼91% peak aerobic power (V̇o2 peak) with tissue sampling from vastus lateralis before (B) and immediately after repetitions 1 (R1), 2 (R2), 9 (R9), and 16 (R16). Eleven untrained volunteers with a V̇o2 peak of 44.3 ± 2.3 ml·kg−1·min−1 participated in the study. Maximal Na+-K+-ATPase activity ( Vmax, in nmol·mg protein−1·h−1) as measured by the 3- O-methylfluorescein K+-stimulated phosphatase assay was reduced ( P < 0.05) by ∼15% with exercise regardless of the number of repetitions performed. In addition, Vmax at R9 and R16 was lower ( P < 0.05) than at R1 and R2. Vanadate-facilitated [3H]ouabain determination of Na+-K+-ATPase content (maximum binding capacity, pmol/g wet wt), although unaltered by exercise, increased ( P < 0.05) 8.3% by R9 with no further increase observed at R16. Assessment of relative changes in isoform abundance measured at B as determined by quantitative immunoblotting showed a 26% increase ( P < 0.05) in the α2-isoform by R2 and a 29% increase in α3 by R9. At R16, β3 was lower ( P < 0.05) than at R2 and R9. No changes were observed in α1, β1, or β2. It is concluded that repeated sessions of heavy exercise, although resulting in increases in the α2- and α3-isoforms and decreases in β3-isoform, also result in depression in maximal catalytic activity.

ENZYMATISCH-FLUOROMETRISCHE BESTIMMUNG DER CORTISOLBINDUNGSKAPAZITÄT DES PLASMA

1967 ◽  
Vol 56 (1) ◽  
pp. 99-106 ◽  
Author(s):  
K. Leybold ◽  
J. Rieper ◽  
L. Weissbecker
Keyword(s):  
Binding Capacity ◽  
Liver Microsomes ◽  
Mean Value ◽  
Female Rats ◽  
Plasma Samples ◽  
Simple Method ◽  
Adult Men ◽  
The Mean ◽  
Precision And Accuracy

ABSTRACT A simple method for the determination of cortisol-binding capacity is described. For saturation of the cortisol-binding proteins, plasma samples are incubated with an excess of cortisol. In the next step NADPH and liver microsomes of female rats are added. The microsomal Δ4-3-ketosteroid hydrogenase only reduces non protein-bound cortisol to tetrahydrocortisol-5α. Then the steroids are extracted by dichloromethane, and after some purification steps analyzed by fluorometry. Tetrahydrocortisol gives practically no fluorescence. The cortisol determined by this method corresponds to protein-bound cortisol and indicates the extent of cortisolbinding capacity. Precision and accuracy of the method were found to be good. The values of cortisol-binding capacity obtained by our method are compared with the results of other authors. The mean value of adult men was 25.5 ± 3.4 μg/100 ml, that of pregnant women, mens IX-X, 42.3 ± 4.2 μg/100 ml.

Sign in / Sign up

Export Citation Format

Share Document