Differentiation of Carnobacterium divergens and Carnobacterium piscicola by numerical analysis of total soluble cell protein patterns and DNA-DNA hybridizations

1995 ◽  
Vol 31 (2) ◽  
pp. 77-79 ◽  
Author(s):  
Leon M. T. Dicks ◽  
Barbara Janssen ◽  
Franco Dellaglio
Keyword(s):  
Numerical Analysis ◽  
Cell Protein ◽  
Protein Patterns ◽  
Soluble Cell ◽  
Carnobacterium Piscicola ◽  
Dna Hybridizations ◽  
Carnobacterium Divergens

scholarly journals Wachstumsgeschwindigkeit der Peptidkette im Tabakmosaikvirus

1967 ◽  
Vol 22 (12) ◽  
pp. 1280-1291 ◽  
Author(s):  
H. Diringer ◽  
F. A. Anderer ◽  
G. Schramm
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Protein Synthesis ◽  
Peptide Chain ◽  
Cell Protein ◽  
Virus Protein ◽  
Animal Cells ◽  
Tobacco Leaves ◽  
Soluble Cell ◽  
C Terminus

The rate of incorporation of labelled amino acids into the complete tobacco mosaic virus (TMV), into soluble virus protein and into soluble cell proteins has been determined in discs of infected and healthy tobacco leaves. The rate of overall protein synthesis is increased by 50% in the infected leaves. At least 60% of the increase derives from the synthesis of virus-specific proteins and the synthesis of cellular proteins is not inhibited. The virus protein synthesis is strongly temperature dependent and shows a maximum at 28 °C.The exchange of free labelled amino acids between the external medium and the inner cellular pool reaches equilibrium within ten minutes. The influence of the exchange rate on the measurement of the kinetics of peptide chain synthesis is discussed in detail.Discs from infected leaves were incubated for short periods at low temperatures in media containing 3H-tyrosine or 3H-proline. Peptides isolated after 5 minutes incubation at 15 °C were found to be uniformly labelled with no apparent gradient of radioactivity from the N- to the C-terminus. The results indicate that the growth rate of the peptide chain at 15 °C is probably higher than 2 - 3 amino acids/sec and at 28 °C higher than 20 amino acids/sec. These values are higher than those for animal cells and similar to those for protein synthesis in Escherichia coli.Comparison of the growth rate of TMV protein with rate of total protein synthesis and the number of ribosomes in the tobacco leaves indicate that only a small portion of the ribosomes takes part in cell protein synthesis.

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