Flow cytometric analysis of marrow cell kinetics in children treated with high-dose MTX and CF rescue

1986 ◽  
Vol 16 (3) ◽  
pp. 277-281 ◽  
Author(s):  
Masahito Tsurusawa ◽  
Kuniaki Sasaki ◽  
Hatsufumi Matsuoka ◽  
Yoshifumi Yamamoto ◽  
Naoyuki Katano ◽  
...  
Keyword(s):  
Cell Kinetics ◽  
Marrow Cell ◽  
Flow Cytometric Analysis ◽  
High Dose ◽  
Flow Cytometric

scholarly journals Cell Kinetics of Regenerating Liver After 70% Hepatectomy in Rats - 2-Color Flow Cytometric Analysis

HPB Surgery ◽  
1992 ◽  
Vol 5 (2) ◽  
pp. 103-115 ◽  
Author(s):  
Jun Tamura ◽  
Junji Tanaka ◽  
Ken-Ichi Fujita ◽  
Masanori Yoshida ◽  
Takayuki Kasamatsu ◽  
...  
Keyword(s):  
Cell Kinetics ◽  
Flow Cytometric Analysis ◽  
Regenerating Liver ◽  
Rat Hepatocyte ◽  
Color Flow ◽  
Flow Cytometric ◽  
Incorporated Brdu ◽  
Fcm Analysis ◽  
Kinetics Of ◽  
Dna Histograms

Two-color flow cytometric (FCM) analysis using anti-bromodeoxyuridine (BrdU) monoclonal antibody (MoAb) was used to investigate the cell kinetics of regenerating liver after 70% partial hepatectomy in rats. Three peaks were seen in DNA histograms of rat hepatocyte nuclei, corresponding to diploid(2c), tetraploid(4c), and octaploid(8c). These proportions changed in the course of regeneration which were clearly demonstrated by DNA histograms using flow cytometry. The proportion of diploid, tetraploid, and octaploid nuclei in control liver were 49.3 ± 1.6%, 45.0 ± 7.4%, and 1.7 ± 0.7%, respectively. A significant change occurred at 24 hours after hepatectomy, as FCM revealed 25.9 ± 1.1% diploid, 54.5 ± 1.2% tetraploid, and 9.0 ± 0.9% octaploid. This shift to polyploid nuclei persisted until 72 hours, and then gradually returned to the pattern of control liver. The S-phase nuclei which incorporated BrdU increased rapidly at 24 hours to a peak of 11.3 ± 0.9%, and gradually decreased to 5.8 ± 0.8%, 5.3 ± 0.8%, 2.4 ± 0.6%, 2.9 ± 1.1%, and 1.2 ± 0.6%, at 48, 72, 96, 120, and 168 hours, respectively. This 2-color FCM analysis made a detailed analysis of the cell kinetics in regenerating hepatocytes possible, and may be applied in investigations of various aspects of liver regeneration.

scholarly journals Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Hui Liu ◽  
Rong Fu ◽  
Yihao Wang ◽  
Hong Liu ◽  
Lijuan Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Protein Extraction ◽  
Flow Cytometric Analysis ◽  
High Dose ◽  
Bone Marrow Mononuclear Cell ◽  
Hemopoietic Cells ◽  
Molecular Weights ◽  
Flow Cytometric ◽  
Immunoglobulin Treatment ◽  
Adrenocortical Hormone

Previously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases. Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia might be mediated by autoantibodies. Autoantibodies were detected on the membrane of various bone marrow (BM) hemopoietic cells by bone marrow mononuclear-cell-Coombs test or flow cytometric analysis. Thus, the hemocytopenia was termed “Immunorelated Pancytopenia” (IRP) to distinguish it from other pancytopenias. Autoantigens in IRP were investigated by membrane protein extraction from BM hemopoietic cells and BM supernatant from IRP patients. Autoantibody IgG was detected in the BM supernatant of 75% of patients (15/20), which was significantly higher than that in aplastic anemia, myelodysplastic syndrome, or autoimmune hemolytic anemia patients (0%) and normal healthy controls (0%) (P<0.01). Autoantigens had approximate molecular weights of 25, 30, 47.5, 60, 65, 70, and 80 kDa, some of which were further identified by mass fingerprinting. This study identified that a G-protein-coupled receptor 156 variant and chain P, a crystal structure of the cytoplasmic domain of human erythrocyte band-3 protein, were autoantigens in IRP. Further studies are needed to confirm the antigenicity of these autoantigens.

scholarly journals Modeling cell survival and change in amount of DNA during protracted irradiation

2016 ◽  
Vol 58 (3) ◽  
pp. 302-312 ◽  
Author(s):  
Yusuke Matsuya ◽  
Kaori Tsutsumi ◽  
Kohei Sasaki ◽  
Yuji Yoshii ◽  
Takaaki Kimura ◽  
...  
Keyword(s):  
Low Dose ◽  
Dose Range ◽  
Flow Cytometric Analysis ◽  
Chinese Hamster ◽  
Clonogenic Survival ◽  
High Dose ◽  
X Rays ◽  
Theoretical Evaluation ◽  
Flow Cytometric ◽  
M Phase

Abstract Hyper-radiosensitivity (HRS) is a well-known bioresponse under low-dose or low-dose-rate exposures. Although disorder of the DNA repair function, non-targeted effects and accumulation of cells in G2 have been experimentally observed, the mechanism for inducing HRS by long-term irradiation is still unclear. On the basis of biological experiments and a theoretical study, we have shown that change in the amount of DNA associated with accumulation of cells in G2 enhances radiosensitivity. To demonstrate continuous irradiation with 250 kVp X-rays, we adopted a fractionated regimen of 0.186 or 1.00 Gy per fraction at intervals of 1 h (i.e. 0.186 Gy/h, 1.00 Gy/h on average) to Chinese Hamster Ovary (CHO)-K1 cells. The change in the amount of DNA during irradiation was quantified by flow cytometric analysis with propidium iodide (PI). Concurrently, we attempted a theoretical evaluation of the DNA damage by using a microdosimetric-kinetic (MK) model that was modified to incorporate the change in the amount of DNA. Our experimental results showed that the fraction of the cells in G2/M phase increased by 6.7% with 0.186 Gy/h and by 22.1% with 1.00 Gy/h after the 12th irradiation. The MK model considering the change in amount of DNA during the irradiation exhibited a higher radiosensitivity at a high dose range, which could account for the experimental clonogenic survival. The theoretical results suggest that HRS in the high dose range is associated with an increase in the total amount of DNA during irradiation.

Segmental Cell Kinetics of the Human Anagen Hair: A DNA-Flow Cytometric Analysis

1990 ◽  
Vol 94 (4) ◽  
pp. 456-460 ◽  
Author(s):  
Franklin Kiesewetter ◽  
Hermann Schell ◽  
Christian Seidel ◽  
Akira Arai ◽  
Otto P. Hornstein
Keyword(s):  
Cell Kinetics ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Anagen Hair ◽  
Kinetics Of

Rapid administration of multiple cycles of high-dose myelosuppressive chemotherapy in patients with metastatic breast cancer.

1993 ◽  
Vol 11 (6) ◽  
pp. 1144-1149 ◽  
Author(s):  
J Crown ◽  
A Kritz ◽  
L Vahdat ◽  
L Reich ◽  
M Moore ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Peripheral Blood ◽  
Flow Cytometric Analysis ◽  
Metastatic Breast ◽  
High Dose Chemotherapy ◽  
High Dose ◽  
Myelosuppressive Chemotherapy ◽  
Flow Cytometric ◽  
Multiple Cycles

PURPOSE To determine the feasibility and safety of a rapidly cycled sequence of high-dose myelosuppressive chemotherapy courses. PATIENTS AND METHODS Seventeen patients with metastatic breast cancer were treated with two courses of cyclophosphamide (CPA; 3.0 g/m2) supported by granulocyte colony-stimulating factor (G-CSF). Following the first CPA treatment, peripheral-blood leukaphereses commenced when the leukocyte count recovered to 1.0 x 10(9)/L. After hematologic recovery from the second dose of CPA, patients were treated with carboplatin 1,500 mg/m2, etoposide 1,200 mg/m2, and CPA 5.0 g/m2 administered over 3 days. The peripheral-blood progenitors (PBPs) were reinfused 3 days later, and G-CSF was recommenced. RESULTS All patients received the three courses. The median interval between treatments was 14 days (range, 13 to 21). Sixteen of the 34 courses of CPA resulted in admissions for fever. Following the third course, neutrophil counts recovered to 0.5 x 10(9)/L at a median of 9 days (range, 8 to 18) after PBP reinfusion and platelets recovered to 50 x 10(9)/L at a median of 12 days (range, 9 to 102). There were no treatment-related deaths. Flow-cytometric analysis was performed on the leukapheresis collections of eight patients. Seven patients with at least 2.0 x 10(6) CD34+ CD33- cells per kilogram body weight exhibited prompt hematologic recovery. One patient with 0.03 x 10(6) CD34+ CD33- cells was still cytopenic on day 21, and required reinfusion of her back-up marrow. Among seven patients with measurable or assessable disease, there were two complete responses (CRs) and four partial responses (PRs). CONCLUSION These preliminary results suggest that multiple, rapidly cycled courses of high-dose myelosuppressive chemotherapy can be administered. PBPs, harvested during the G-CSF-augmented rebound from CPA-induced cytopenia, produce rapid hematologic recovery in patients undergoing high-dose chemotherapy (HDC). Further follow-up will be necessary to assess the efficacy of this specific regimen in the treatment of metastatic breast cancer.

Cell kinetics in a model of artificial skin. An immunohistochemical and flow cytometric analysis

10.4081/1622 ◽  
2009 ◽  
Vol 45 (2) ◽  
pp. 125 ◽  
Author(s):  
A Casasco ◽  
M Casasco ◽  
A Icaro Cornaglia ◽  
N Zerbinati ◽  
G Mazzini ◽  
...  
Keyword(s):  
Cell Kinetics ◽  
Flow Cytometric Analysis ◽  
Artificial Skin ◽  
Flow Cytometric
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