Histone H1 in the centromeric heterochromatin of Glyptotendipes barbipes larval polytene chromosomes

Chromosoma ◽  
1989 ◽  
Vol 98 (1) ◽  
pp. 64-68 ◽  
Author(s):  
S. G. Nonchev ◽  
P. V. Michailova ◽  
C. D. Venkov ◽  
R. G. Tsanev
Keyword(s):  
Polytene Chromosomes ◽  
Histone H1 ◽  
Centromeric Heterochromatin

Bands, interbands and puffs in native Drosophila polytene chromosomes are recognized by a monoclonal antibody to an epitope in the carboxy-terminal tail of histone H1

Chromosoma ◽  
1989 ◽  
Vol 98 (6) ◽  
pp. 411-421 ◽  
Author(s):  
Ronald J. Hill ◽  
Fujiko Watt ◽  
Catherine M. Wilson ◽  
Theodora Fifis ◽  
P. Anne Underwood ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Polytene Chromosomes ◽  
Histone H1 ◽  
Carboxy Terminal

scholarly journals The Drosophila salivary gland chromocenter contains highly polytenized subdomains of mitotic heterochromatin.

Genetics ◽  
1995 ◽  
Vol 139 (2) ◽  
pp. 659-670 ◽  
Author(s):  
P Zhang ◽  
A C Spradling
Keyword(s):  
Salivary Gland ◽  
Dna Sequences ◽  
Polytene Chromosomes ◽  
Centromeric Heterochromatin ◽  
Mitotic Chromosomes ◽  
Satellite Dnas ◽  
Central Mass ◽  
P Elements

Abstract Peri-centromeric regions of Drosophila melanogaster chromosomes appear heterochromatic in mitotic cells and become greatly underrepresented in giant polytene chromosomes, where they aggregate into a central mass called the chromocenter. We used P elements inserted at sites dispersed throughout much of the mitotic heterochromatin to analyze the fate of 31 individual sites during polytenization. Analysis of DNA sequences flanking many of these elements revealed that middle repetitive or unique sequence DNAs frequently are interspersed with satellite DNAs in mitotic heterochromatin. All nine Y chromosome sites tested were underrepresented > 20-fold on Southern blots of polytene DNA and were rarely or never detected by in situ hybridization to salivary gland chromosomes. In contrast, nine tested insertions in autosomal centromeric heterochromatin were represented fully in salivary gland DNA, despite the fact that at least six were located proximal to known blocks of satellite DNA. The inserted sequences formed diverse, site-specific morphologies in the chromocenter of salivary gland chromosomes, suggesting that domains dispersed at multiple sites in the centromeric heterochromatin of mitotic chromosomes contribute to polytene beta-heterochromatin. We suggest that regions containing heterochromatic genes are organized into dispersed chromatin configurations that are important for their function in vivo.

A standard cytogenetic map for Anopheles sundaicus (Diptera: Culicidae) and evidence for chromosomal differentiation in populations from Thailand and Indonesia

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 165-173 ◽  
Author(s):  
S. Sukowati ◽  
V. Baimai
Keyword(s):  
Karyotype Analysis ◽  
Polytene Chromosomes ◽  
Centromeric Heterochromatin ◽  
Chromosome X ◽  
Cytogenetic Map ◽  
Banding Patterns ◽  
Chromosome Y ◽  
Central Java ◽  
Chromosomal Differentiation ◽  
North Sumatra

A standard photographic map of polytene chromosomes of Anopheles sundaicus was constructed from ovarian nurse cells and is described herein. Polytene chromosomes of wild specimens collected from 9 different geographical areas in Thailand and Indonesia have been analyzed. Specimens from these populations appear to share banding patterns with standard gene arrangements, except for some specimens from Purworejo, in Central Java, and South Tapanuli and Asahan, both of North Sumatra, which exhibited distinct banding patterns at the tip of chromosome X (Xb) compared with the standard sequence (Xa). Moreover, some specimens collected from Asahan, North Sumatra, consistently showed distinct loosely diffuse bands in zone 19 of chromosome arm 2R (2Rb) compared with the standard banding patterns (2Ra). The existence of the 2Rb pattern correlates perfectly with the presence of an extra block of centromeric heterochromatin in autosome 2 as revealed by mitotic karyotype analysis (2n = 6). These cytological differences have led to the recognition of 3 distinct forms, viz., A, B, and C, within the taxon An. sundaicus. In addition, forms A and C show a normal size for chromosome Y, (Y1), while form B has a relatively larger type of chromosome Y, (Y2). Form A is widely distributed in Thailand and Indonesia, while form B has been found in North Sumatra and Central Java. Form C, however, has been found only in Asahan, North Sumatra. Key words : Anopheles sundaicus, polytene chromosome map, mitotic karyotype, chromosomal differentiation.

The Control of Phosphate Content of Histone H1

1979 ◽  
Vol 7 (1) ◽  
pp. 229-229
Author(s):  
HARRY MATTHEWS
Keyword(s):  
Histone H1 ◽  
Phosphate Content
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