Nonrandom distribution of methotrexate-induced aberrations on human chromosomes. Detection of further folic acid sensitive fragile sites

G. Barbi; P. Steinbach; W. Vogel

doi:10.1007/bf00292586

Nonrandom distribution of methotrexate-induced aberrations on human chromosomes. Detection of further folic acid sensitive fragile sites

Human Genetics ◽

10.1007/bf00292586 ◽

1984 ◽

Vol 68 (4) ◽

pp. 290-294 ◽

Cited By ~ 37

Author(s):

G. Barbi ◽

P. Steinbach ◽

W. Vogel

Keyword(s):

Folic Acid ◽

Fragile Sites ◽

Human Chromosomes ◽

Nonrandom Distribution

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On the nature of folic-acid-sensitive fragile sites in human chromosomes: An hypothesis

American Journal of Medical Genetics ◽

10.1002/ajmg.1320160105 ◽

1983 ◽

Vol 16 (1) ◽

pp. 23-28 ◽

Cited By ~ 27

Author(s):

Carlos L. Krumdieck ◽

Patricia N. Howard-Peebles ◽

John M. Opitz

Keyword(s):

Folic Acid ◽

Fragile Sites ◽

Human Chromosomes

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A Novel Technique for Observing the Internal Ultrastructure of Human Chromosomes with Known Karyotype

Microscopy and Microanalysis ◽

10.1017/s143192760808063x ◽

2008 ◽

Vol 14 (4) ◽

pp. 357-361 ◽

Cited By ~ 2

Author(s):

Mohammad Ghazizadeh ◽

Yoshihiro Sasaki ◽

Tatsuo Oguro ◽

Shigeru Sato ◽

Seiko Egawa ◽

...

Keyword(s):

Metaphase Chromosome ◽

Osmium Tetroxide ◽

Human Lymphocytes ◽

Fragile Sites ◽

Human Chromosomes ◽

Chromosome Research ◽

Novel Technique ◽

Transmission Electron ◽

Comprehensive Information ◽

Normal Human

Observation of the internal ultrastructure of human chromosomes by transmission electron microscopy (TEM) has frequently been attempted in spite of the difficulties in detaching metaphase chromosome spreads from the glass slide for further processing. In this study we have used a method in which metaphase chromosome spreads were prepared on a flexible thermoplastic membrane (ACLAR) film. To assess chromosome identity, a diamidino-phenylindole staining and karyotying was first done using a conventional cytogenetic system. The chromosome spreads were then fixed with 1% osmium tetroxide, stained with freshly prepared 2% tannic acid, dehydrated, and flat-embedded in epoxy resin. The resin sheet was easily detachable and carried whole chromosome spreads. By this method, TEM observation of chromosomes from normal human lymphocytes allowed a thorough examination of the ultrastructure of centromeres, telomeres, fragile sites, and other chromosomal regions. Various ultrastructural patterns including thick electron dense boundaries, less dense internal regions, and extended chromatin loops at the periphery of the chromosomes were discernible. Application of the present method to chromosome research is expected to provide comprehensive information on the internal ultrastructure of different chromosomal regions in relation to function.

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