Characterization of extrachromosomal DNA in the flesh fly Sarcophaga bullata

Chromosoma ◽  
1979 ◽  
Vol 75 (2) ◽  
pp. 145-159 ◽  
Author(s):  
David Samols ◽  
Hewson Swift
Keyword(s):  
Extrachromosomal Dna ◽  
Flesh Fly ◽  
Sarcophaga Bullata

Characterization of phenoloxidases from larval cuticle of Sarcophaga bullata and a comparison with cuticular enzymes from other species

1987 ◽  
Vol 65 (5) ◽  
pp. 1158-1166 ◽  
Author(s):  
F. Michael Barrett
Keyword(s):  
Musca Domestica ◽  
Sodium Azide ◽  
Lucilia Sericata ◽  
Phormia Regina ◽  
Ph Optimum ◽  
Larval Cuticle ◽  
Sarcophaga Bullata ◽  
Tyrosinase Enzyme ◽  
Laccase Enzyme

A tyrosinase, enzyme A, and a laccase, enzyme B, have been partially purified from larval cuticle of the flesh fly Sarcophaga bullata. Enzyme A (EC 1.10.3.1, o-diphenol: O2 oxidoreductase) oxidizes o-diphenols but not p-diphenols, is strongly inhibited by phenylthiourea, and has a pH optimum around pH 6.5–7.0. Assays on intact cuticle suggest that it becomes maximally activated at pH between 8 and 9. Enzyme B (EC 1.10.3.2, p-diphenol: O2 oxidoreductase) oxidizes both o-diphenols and p-diphenols, is not inhibited by phenylthiourea but is inhibited by concentrations of sodium azide that have little effect on enzyme A, and has a pH optimum near pH 4.5. Enzyme A was identified in extracts of cuticle from nine other species representing five orders. Enzyme B was much less readily extractable but was partially purified from larval cuticle of Phormia regina, Musca domestica, and Lucilia sericata. A summary of all species studied to date makes possible the test of a hypothesis about the distribution of these cuticular phenoloxidases within the Insecta.

scholarly journals Histochemical localization of trehalase activity in dorsal flight muscle of the flesh fly Sarcophaga bullata with light and electron microscopy.

1975 ◽  
Vol 23 (11) ◽  
pp. 800-807 ◽  
Author(s):  
P L Chang ◽  
P E Morrison
Keyword(s):  
Electron Microscopy ◽  
Incubation Medium ◽  
Mitochondrial Membrane ◽  
Flight Muscle ◽  
Light And Electron Microscopy ◽  
Inner Mitochondrial Membrane ◽  
Electron Microscopic ◽  
Flesh Fly ◽  
Trehalase Activity ◽  
Sarcophaga Bullata

Trehalase activity in flight muscle of the flesh fly Sacrophaga bullata is detected histochemically at light- and electron-microscopic levels by using diaminobenzidine, glucose oxidase and peroxidase in the incubation medium. The association of trehalase activity with the inner mitochondrial membrane is confirmed. Biochemical assay shows that about 50% of the initial total trehalase activity is lost from the tissue during the histochemical processing and about 50% remains for histochemical detection.

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