Characterization of repetitive DNA in rye (Secale cereale)

Chromosoma ◽  
1974 ◽  
Vol 48 (4) ◽  
pp. 427-440 ◽  
Author(s):  
P. K. Ranjekar ◽  
J. G. Lafontaine ◽  
D. Pallotta
Keyword(s):  
Repetitive Dna ◽  
Secale Cereale

Wheat specific repetitive DNA sequences ? construction and characterization of four different genomic clones

1986 ◽  
Vol 72 (2) ◽  
pp. 207-210 ◽  
Author(s):  
M. Metzlaff ◽  
W. Troebner ◽  
F. Baldauf ◽  
R. Schlegel ◽  
J. Cullum
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Genomic Clones

scholarly journals Genetic Diversity of Xanthomonas campestris pv. vitians, the Causal Agent of Bacterial Leafspot of Lettuce

2003 ◽  
Vol 93 (5) ◽  
pp. 596-603 ◽  
Author(s):  
Jeri D. Barak ◽  
Robert L. Gilbertson
Keyword(s):  
Genetic Diversity ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Plasmid Dna ◽  
Xanthomonas Campestris ◽  
Rflp Analysis ◽  
Dna Probe ◽  
Internal Transcribed Spacer Region ◽  
Geographical Regions

Bacterial leafspot of lettuce (BLS), caused by Xanthomonas campes-tris pv. vitians, has become more prevalent in many lettuce-growing areas of the world over the past decade. To gain insight into the nature of these outbreaks, the genetic variation in X. campestris pv. vitians strains from different geographical locations was examined. All strains were first tested for pathogenicity on lettuce plants, and then genetic diversity was assessed using (i) gas-chromatographic analysis of bacterial fatty acids, (ii) polymerase chain reaction analysis of repetitive DNA sequences (rep-PCR), (iii) DNA sequence analysis of the internal transcribed spacer region 1 (ITS1) of the ribosomal RNA, (iv) restriction fragment length polymorphism (RFLP) analysis of total genomic DNA with a repetitive DNA probe, and (v) detection and partial characterization of plasmid DNA. Fatty acid analysis identified all pathogenic strains as X. campestris, but did not consistently identify all the strains as X. campestris pv. vitians. The rep-PCR fingerprints and ITS1 sequences of all pathogenic X. campestris pv. vitians strains examined were identical, and distinct from those of the other X. campestris pathovars. Thus, these characteristics did not reveal genetic diversity among X. campestris pv. vitians strains, but did allow for differentiation of X. campestris pathovars. Genetic diversity among X. campestris pv. vitians strains was revealed by RFLP analysis with a repetitive DNA probe and by characterization of plasmid DNA. This diversity was greatest among strains from different geographical regions, although diversity among strains from the same location also was detected. The results of this study suggest that these X. campestris pv. vitians strains are not clonal, but comprise a relatively homogeneous group.

scholarly journals Correction to: low coverage sequencing for repetitive DNA analysis in Passiflora edulis Sims: citogenomic characterization of transposable elements and satellite DNA

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Vanessa Carvalho Cayres Pamponét ◽  
Margarete Magalhães Souza ◽  
Gonçalo Santos Silva ◽  
Fabienne Micheli ◽  
Cláusio Antônio Ferreira de Melo ◽  
...  
Keyword(s):  
Transposable Elements ◽  
Repetitive Dna ◽  
Satellite Dna ◽  
Dna Analysis ◽  
Passiflora Edulis ◽  
Passiflora Edulis Sims ◽  
Low Coverage

Characterization of two repetitive DNA families (RrS1 and Rana/Pol III) in the genomes of Palaearctic green water frogs

1999 ◽  
Vol 66 (3) ◽  
pp. 255-263 ◽  
Author(s):  
Matilde Ragghianti ◽  
Stefania Bucci ◽  
Francesca Guerrini ◽  
Giorgio Mancino
Keyword(s):  
Repetitive Dna ◽  
Green Water ◽  
Water Frogs ◽  
Pol Iii

Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1061-1069 ◽  
Author(s):  
A. Cuadrado ◽  
N. Jouve ◽  
C. Ceoloni
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
5S Rdna ◽  
Individual Identification ◽  
Highly Repetitive Dna ◽  
The Individual

The molecular characterization of heterochromatin in six lines of rye has been performed using fluorescence in situ hybridization (FISH). The highly repetitive rye DNA sequences pSc 119.2, pSc74, and pSc34, and the probes pTa71 and pSc794 containing the 25S–5.8S–18S rDNA (NOR) and the 5S rDNA multigene families, respectively, were used. This allowed the individual identification of all seven rye chromosomes and most chromosome arms in all lines. All varieties showed similar but not identical patterns. A standard in situ hybridization map was constructed following the nomenclature system recommended for C-bands. All FISH sites observed appeared to correspond well with C-band locations, but not all C-banding sites coincided with hybridization sites of the repetitive DNA probes used. Quantitative and qualitative differences between different varieties were found for in situ hybridization response at corresponding sites. Variation between plants and even between homologous chromosomes of the same plant was found in open-pollinated lines. In inbred lines, the in situ pattern of the homologues was practically identical and no variation between plants was detected. The observed quantitative and qualitative differences are consistent with a corresponding variation for C-bands detected both within and between cultivars.Key words: fluorescence in situ hybridization, repetitive DNA, rye, Secale cereale, polymorphism.

scholarly journals Identification and VIGS-based characterization of Bx1 ortholog in rye (Secale cereale L.)

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171506 ◽  
Author(s):  
Jolanta Groszyk ◽  
Mariusz Kowalczyk ◽  
Yuliya Yanushevska ◽  
Anna Stochmal ◽  
Monika Rakoczy-Trojanowska ◽  
...  
Keyword(s):  
Secale Cereale ◽  
Secale Cereale L

Characterization of a repetitive DNA element from Entamoeba histolytica

1992 ◽  
Vol 51 (1) ◽  
pp. 165-167 ◽  
Author(s):  
Bertha Michel ◽  
Alejandro Alagón ◽  
Paul M. Lizardi ◽  
Mario Zurita
Keyword(s):  
Entamoeba Histolytica ◽  
Repetitive Dna

scholarly journals In Depth Characterization of Repetitive DNA in 23 Plant Genomes Reveals Sources of Genome Size Variation in the Legume Tribe Fabeae

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143424 ◽  
Author(s):  
Jiří Macas ◽  
Petr Novák ◽  
Jaume Pellicer ◽  
Jana Čížková ◽  
Andrea Koblížková ◽  
...  
Keyword(s):  
Genome Size ◽  
Repetitive Dna ◽  
Size Variation ◽  
Genome Size Variation ◽  
Plant Genomes
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