The actin loci in the genus Drosophila: establishment of chromosomal homologies among distantly related species by in situ hybridization

Chromosoma ◽  
1986 ◽  
Vol 94 (4) ◽  
pp. 297-308 ◽  
Author(s):  
Michael Loukas ◽  
Fotis C. Kafatos
Keyword(s):  
In Situ Hybridization ◽  
Related Species ◽  
Chromosomal Homologies

scholarly journals UNDER-REPLICATION OF RIBOSOMAL CISTRONS IN POLYTENE CHROMOSOMES OF RHYNCHOSCIARA

1974 ◽  
Vol 62 (1) ◽  
pp. 215-222 ◽  
Author(s):  
A. G. Gambarini ◽  
F. J. S. Lara
Keyword(s):  
Salivary Gland ◽  
In Situ Hybridization ◽  
Xenopus Laevis ◽  
Related Species ◽  
Polytene Chromosomes ◽  
Chromosome X ◽  
Heterologous Hybridization ◽  
Ribosomal Cistrons ◽  
Rhynchosciara Americana

DNA preparations obtained from several tissues of Rhynchosciara americana and two related species, R. milleri and R. papaveroi, were hybridized to R. americana rRNA. The percentage of hybridization was found to be higher in tissues with low polyteny than in tissues with high polyteny, suggesting a relationship between the amount of rDNA and the tissue polyteny. This could be explained by under-replication of ribosomal cistrons in polytene cells, such as those from the salivary gland. Only slight tissue-dependent changes in the percentages of hybridization can be observed in heterologous hybridization using Xenopus laevis rRNA. The possibility that these experiments could not detect differences in the amount of ribosomal cistrons among tissues is discussed. The female:male ratio for the percentages of hybridization in the salivary gland of R. americana agrees with the results obtained by in situ hybridization experiments (16, 17) which have shown that the rRNA cistrons are distributed among chromosomes other than chromosome X.

Tandem repetitive Afa-family sequences from Leymus racemosus and Psathyrostachys juncea (Poaceae)

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1258-1260 ◽  
Author(s):  
Kiyotaka Nagaki ◽  
Masahiro Kishii ◽  
Hisashi Tsujimoto ◽  
Tetsuo Sasakuma
Keyword(s):  
In Situ Hybridization ◽  
Phylogenetic Tree ◽  
Tandem Repeat ◽  
Related Species ◽  
Perennial Species ◽  
Elymus Trachycaulus ◽  
Psathyrostachys Juncea ◽  
Leymus Racemosus ◽  
Tribe Triticeae

Tandem repetitive Afa-family sequences of 340 bp are known to occur in wheat and related species of tribe Triticeae. We isolated six and three Afa-family sequences from Leymus racemosus and Psathyrostachys juncea, respectively, both of which are perennial species. The sequences account for 0.5% and 0.2% of L. racemosus and P. juncea genomes, respectively, and using in situ hybridization were located in subtelomeric and interstitial regions of L. racemosus chromosomes. These sequences are clustered with those of Elymus trachycaulus in the phylogenetic tree. Our findings indicate that the Afa-family sequences have been amplified at least twice in the lineage of L. racemosus, P. juncea, and E. trachycaulus.Key words: Triticeae, Leymus, Psathyrostachys, tandem repeat, Afa-family sequences.

scholarly journals In situ hybridization analysis of chromosomal homologies in Drosophila melanogaster and Drosophila virilis.

Genetics ◽  
1989 ◽  
Vol 122 (1) ◽  
pp. 99-109 ◽  
Author(s):  
J H Whiting ◽  
M D Pliley ◽  
J L Farmer ◽  
D E Jeffery
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
In Situ Hybridization ◽  
Recombinant Dna ◽  
Unique Sequence ◽  
Drosophila Virilis ◽  
Multigene Families ◽  
Larval Salivary Gland ◽  
Chromosomal Homologies

Abstract Twenty-four biotin-labeled recombinant-DNA probes which contained putative unique-sequence Drosophila melanogaster DNA were hybridized to larval salivary-gland chromosomes of D. melanogaster and Drosophila virilis. All probes hybridized to D. melanogaster chromosomes at the expected sites. However, one probe hybridized to at least 16 additional sites, and one hybridized to one additional site. Thirteen probes hybridized strongly to D. virilis chromosomes, four hybridized weakly and infrequently, and seven did not hybridize. Probes representing two multigene families (beta-tubulin and yolk-protein) hybridized as would be expected if all sites had been conserved in the two species on the same chromosomal elements. The multiple hybridization sites of a third probe which may represent a multigene family were also conserved. The results were consistent with H.J. Muller's proposal that chromosomal elements have been conserved during evolution of this genus.

The actin loci in the genus Drosophila: establishment of chromosomal homologies among five nearctic species of the Drosophila obscura group by in situ hybridization

Chromosoma ◽  
2002 ◽  
Vol 111 (4) ◽  
pp. 256-266 ◽  
Author(s):  
George P. Bondinas ◽  
Michael G. Loukas ◽  
George N. Goulielmos ◽  
Diether Sperlich
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Homologies ◽  
Obscura Group

scholarly journals Differentiation of Muller's Chromosomal Elements D and E in the Obscura Group of Drosophila

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 139-146
Author(s):  
Carmen Segarra ◽  
Griselda Ribó ◽  
Montserrat Aguadé
Keyword(s):  
In Situ Hybridization ◽  
Homologous Chromosome ◽  
Genomic Library ◽  
Pairwise Comparisons ◽  
Species Divergence ◽  
Chromosomal Homologies ◽  
Obscura Group

Abstract Twenty-two markers located on Muller's elements D or E have been mapped by in situ hybridization in six species of the obscura group of Drosophila and in D. melanogaster. The obscura species can be grouped into a Palearctic cluster (D. subobscura, D. madeirensis and D. guanche) and a Nearctic one (D. pseudoobscura, D. persimilis and D. miranda). Eleven of the probes contain known genes: E74, Acp70A, Est-5, hsp28/23, hsp83, emc, hsp70, Xdh, Acph-I, Cec and rp49. The remaining probes are recombinant phages isolated from a D. subobscura genomic library. All these markers hybridize to the putative homologous chromosome or chromosomal arm of elements D and E. Thus, these elements have conserved their genic content during species divergence. Chromosomal homologies proposed previously for each element among the species of the same cluster have been compared with the present results. The distribution of markers within each element has changed considerably as inferred from pairwise comparisons of obscura species included in the two different clusters. Only chromosomal segments defined by closely linked markers have been conserved: one such segment has been detected in element D and three in element E between D. subobscura and D. pseudoobscura.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

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