Organization and characterization of three genes involved in d-xylose catabolism in Lactobacillus pentosus

1991 ◽  
Vol 230 (1-2) ◽  
pp. 161-169 ◽  
Author(s):  
B. Christien Lokman ◽  
Pieter van Santen ◽  
Jan C. Verdoes ◽  
Jaap Krüse ◽  
Rob J. Leer ◽  
...  
Keyword(s):  
Lactobacillus Pentosus ◽  
Xylose Catabolism

Optimization of extraction conditions and fatty acid characterization of Lactobacillus pentosus cell-bound biosurfactant/bioemulsifier

2014 ◽  
Vol 95 (2) ◽  
pp. 313-320 ◽  
Author(s):  
Xanel Vecino ◽  
Letricia Barbosa-Pereira ◽  
Rosa Devesa-Rey ◽  
José M Cruz ◽  
Ana B Moldes
Keyword(s):  
Fatty Acid ◽  
Lactobacillus Pentosus

Expression and biochemical characterization of a new alkaline tannase from Lactobacillus pentosus

2019 ◽  
Vol 157 ◽  
pp. 36-41 ◽  
Author(s):  
Apinun Kanpiengjai ◽  
Kridsada Unban ◽  
Thu-Ha Nguyen ◽  
Dietmar Haltrich ◽  
Chartchai Khanongnuch
Keyword(s):  
Biochemical Characterization ◽  
Lactobacillus Pentosus

scholarly journals Partial Characterization of Biosurfactant fromLactobacillus pentosusand Comparison with Sodium Dodecyl Sulphate for the Bioremediation of Hydrocarbon Contaminated Soil

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
A. B. Moldes ◽  
R. Paradelo ◽  
X. Vecino ◽  
J. M. Cruz ◽  
E. Gudiña ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Contaminated Soil ◽  
Sodium Dodecyl Sulphate ◽  
Day Treatment ◽  
Sodium Dodecyl ◽  
Carbonyl Groups ◽  
Lactobacillus Pentosus ◽  
Peptide Linkage ◽  
A Cell

The capability of a cell bound biosurfactant produced byLactobacillus pentosus, to accelerate the bioremediation of a hydrocarbon-contaminated soil, was compared with a synthetic anionic surfactant (sodium dodecyl sulphate SDS-). The biosurfactant produced by the bacteria was analyzed by Fourier transform infrared spectroscopy (FTIR) that clearly indicates the presence of OH and NH groups, C=O stretching of carbonyl groups and NH nebding (peptide linkage), as well as CH2–CH3and C–O stretching, with similar FTIR spectra than other biosurfactants obtained from lactic acid bacteria. After the characterization of biosurfactant by FTIR, soil contaminated with 7,000 mg Kg−1of octane was treated with biosurfactant fromL. pentosusor SDS. Treatment of soil for 15 days with the biosurfactant produced byL. pentosusled to a 65.1% reduction in the hydrocarbon concentration, whereas SDS reduced the octane concentration to 37.2% compared with a 2.2% reduction in the soil contaminated with octane in absence of biosurfactant used as control. Besides, after 30 days of incubation soil with SDS or biosurfactant gave percentages of bioremediation around 90% in both cases. Thus, it can be concluded that biosurfactant produced byL. pentosusaccelerates the bioremediation of octane-contaminated soil by improving the solubilisation of octane in the water phase of soil, achieving even better results than those reached with SDS after 15-day treatment.

Lactobacillus pentosus B231 Isolated from a Portuguese PDO Cheese: Production and Partial Characterization of Its Bacteriocin

2014 ◽  
Author(s):  
Joana Guerreiro ◽  
Vitor Monteiro ◽  
Carla Ramos ◽  
Bernadette Dora Gombossy de Melo Franco ◽  
Rafael Chacon Ruiz Martinez ◽  
...  
Keyword(s):  
Partial Characterization ◽  
Lactobacillus Pentosus ◽  
Cheese Production

Cloning and characterization of two distinct water-forming NADH oxidases from Lactobacillus pentosus for the regeneration of NAD

2016 ◽  
Vol 39 (4) ◽  
pp. 603-611 ◽  
Author(s):  
Jian-Dong Zhang ◽  
Zhi-Mei Cui ◽  
Xiao-Jun Fan ◽  
Hua-Lei Wu ◽  
Hong-Hong Chang
Keyword(s):  
Lactobacillus Pentosus

scholarly journals Cloning, Sequence Analysis, and Characterization of the Genes Involved in Isoprimeverose Metabolism in Lactobacillus pentosus

1998 ◽  
Vol 180 (9) ◽  
pp. 2312-2320 ◽  
Author(s):  
Stéphane Chaillou ◽  
B. Christien Lokman ◽  
Rob J. Leer ◽  
Clara Posthuma ◽  
Pieter W. Postma ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Catabolite Repression ◽  
Promoter Region ◽  
Membrane Fraction ◽  
Wild Type ◽  
Lactobacillus Pentosus ◽  
Repressor Gene ◽  
Hydrolysis Of ◽  
Xylosidase Activity

ABSTRACT Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon,xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylRbinding site (xylO) and a cre-like element, mediating CcpA-dependent catabolite repression, were found in the promoter region. L. pentosus mutants in which bothxylP and xylQ (LPE1) or only xylQ(LPE2) was inactivated retained the ability to ferment xylose but were impaired in their ability to ferment isoprimeverose (α-d-xylopyranosyl-(1,6)-d-glucopyranose). Disruption of xylQ resulted specifically in the loss of a membrane-associated α-xylosidase activity when LPE1 or LPE2 cells were grown on xylose. In the membrane fraction of wild-type bacteria, α-xylosidase could catalyze the hydrolysis of isoprimeverose andp-nitrophenyl-α-d-xylopyranoside with apparent Km and V maxvalues of 0.2 mM and 446 nmol/min/mg of protein, and 1.3 mM and 54 nmol/min/mg of protein, respectively. The enzyme could also hydrolyze the α-xylosidic linkage in xyloglucan oligosaccharides, but neither methyl-α-d-xylopyranoside nor α-glucosides were substrates. Glucose repressed the synthesis of α-xylosidase fivefold, and 80% of this repression was released in an L. pentosus ΔccpA mutant. The α-xylosidase gene was also expressed in the absence of xylose when xylR was disrupted.

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