scholarly journals Chromosomal localization of human homologs of the Drosophila heterochromatin protein 1 (HP1) gene

1993 ◽  
Vol 4 (2) ◽  
pp. 124-126 ◽  
Author(s):  
Christophe Chevillard ◽  
Wolf Reik ◽  
Mickael Mc Dermott ◽  
Michel Fontes ◽  
Marie G. Mattei ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Heterochromatin Protein 1 ◽  
Heterochromatin Protein ◽  
Drosophila Heterochromatin ◽  
Drosophila Heterochromatin Protein

Mapping of two human homologs of a Drosophila heterochromatin protein gene to the X Chromosome

1992 ◽  
Vol 3 (11) ◽  
pp. 650-652 ◽  
Author(s):  
Wolf Reik ◽  
Margaret A. Leversha ◽  
Nick R. Waterfield ◽  
Prim B. Singh
Keyword(s):  
X Chromosome ◽  
Protein Gene ◽  
Heterochromatin Protein ◽  
Drosophila Heterochromatin ◽  
Drosophila Heterochromatin Protein

scholarly journals Molecular cloning of a human homologue of Drosophila heterochromatin protein HP1 using anti-centromere autoantibodies with anti-chromo specificity

1993 ◽  
Vol 104 (2) ◽  
pp. 573-582 ◽  
Author(s):  
W.S. Saunders ◽  
C. Chue ◽  
M. Goebl ◽  
C. Craig ◽  
R.F. Clark ◽  
...  
Keyword(s):  
Epitope Mapping ◽  
Structural Motif ◽  
Amino Terminus ◽  
Cdna Clones ◽  
Human Homologue ◽  
Expression Library ◽  
Heterochromatin Protein ◽  
Chromosomal Proteins ◽  
Drosophila Heterochromatin ◽  
Drosophila Heterochromatin Protein

We have identified a novel autoantibody specificity in scleroderma that we term anti-chromo. These antibodies recognize several chromosomal antigens with apparent molecular mass of between 23 and 25 kDa, as determined by immunoblots. Anti-chromo autoantibodies occur in 10–15% of sera from patients with anti-centromere antibodies (ACA). We used anti-chromo antibodies to screen a human expression library and obtained cDNA clones encoding a 25 kDa chromosomal autoantigen. DNA sequence analysis reveals this protein to be a human homologue of HP1, a heterochromatin protein of Drosophila melanogaster. We designate our cloned protein HP1Hs alpha. Epitope mapping experiments using both human and Drosophila HP1 reveal that anti-chromo antibodies target a region at the amino terminus of the protein. This region contains a conserved motif, the chromo domain (or HP1/Pc box), first recognized by comparison of Drosophila HP1 with the Polycomb gene product. Both proteins are thought to play a role in creating chromatin structures in which gene expression is suppressed. Anti-chromo thus defines a novel type of autoantibody that recognizes a conserved structural motif found on a number of chromosomal proteins.

Sign in / Sign up

Export Citation Format

Share Document