Heavy metal tolerant transgenic Brassica napus L. and Nicotiana tabacum L. plants

1989 ◽  
Vol 78 (2) ◽  
pp. 161-168 ◽  
Author(s):  
S. Misra ◽  
L. Gedamu
Keyword(s):  
Heavy Metal ◽  
Brassica Napus ◽  
Nicotiana Tabacum ◽  
Brassica Napus L ◽  
Nicotiana Tabacum L

scholarly journals Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn

2008 ◽  
Vol 53 (No. 1) ◽  
pp. 7-15 ◽  
Author(s):  
M. Turan ◽  
A. Esringü
Keyword(s):  
Heavy Metal ◽  
Brassica Napus ◽  
Metal Uptake ◽  
Indian Mustard ◽  
Metal Availability ◽  
Heavy Metal Uptake ◽  
Brassica Napus L ◽  
Plant Parts ◽  
Spiked Soil ◽  
Soil Phytoremediation

The use of plants to remove heavy metals from soil (phytoremediation) is expanding due to its cost-effectiveness as compared to conventional methods and it has revealed a great potential. Since contaminants such as Pb or Cd have a limited bioavailability in the soil, methods to facilitate their transport to the shoots and roots of plants are required for successful phytoremediation. The objective of this study was to investigate the effects of addition of different rates (0, 3, 6 and 12 mmol/kg) of ethylene diaminetetraacetate (EDTA) on heavy metal availability in soils contaminated with 50 mg/kg Cd (CdCl<sub>2</sub>), 50 mg/kg Cu (CuSO<sub>4</sub>), 50 mg/kg Pb [Pb(NO<sub>3</sub>)<sub>2</sub>] and 50 mg/kg Zn (ZnSO<sub>4</sub>), and on the capacity of canola (Brassica napus L.) and Indian mustard (Brassica junceaL.) plants to uptake Cu, Cd, Pb and Zn in a growth chamber. Results indicated that EDTA application increased heavy metal availability and uptake by plants. Significant differences were obtained in both species and plant parts. As for plant species tested, canola was more effective in the uptake of Cu, Cd, Pb and Zn. Root heavy metal uptake of both species was higher than shoot heavy metal uptake.

scholarly journals Cadmium and zinc induced similar changes in protein and glycoprotein patterns in tobacco (nicotiana tabacum l.) seedlings and plants

2012 ◽  
Vol 63 (3) ◽  
pp. 321-335 ◽  
Author(s):  
Petra Peharec Štefanić ◽  
Sandra Šikić ◽  
Petra Cvjetko ◽  
Biljana Balen
Keyword(s):  
Heavy Metal ◽  
Nicotiana Tabacum ◽  
Protein Pattern ◽  
Heavy Metal Stress ◽  
Toxic Element ◽  
Total Soluble Protein ◽  
Nicotiana Tabacum L ◽  
Tobacco Seedlings ◽  
The Difference ◽  
Adult Plants

The effects of 10 μmol L-1 and 15 μmol L-1 cadmium (Cd), a nonessential toxic element and 25 μmol L-1 and 50 μmol L-1 zinc (Zn), an essential micronutrient, on proteins and glycoproteins of Nicotiana tabacum L. seedlings and plants were investigated after exposure to each metal alone or to their combinations. Changes in only few polypeptides related to heavy metal treatments were observed in tobacco seedlings and leaves of adult plants, while the greatest change in total soluble protein pattern was observed in plant roots. Differences between control and treated tobacco tissues were more pronounced in the glycoprotein pattern, which was analysed by application of different lectins. The majority of the detected glycoproteins in leaves and roots of adult plants can be considered as a result of enhanced glycosylation due to heavy metal stress. The difference in glycoproteins between Cd and Zn application on tobacco seedlings and adult plants could not be determined since enhanced glycosylation was noticed after treatment with either metal alone or in combination. Therefore, it can be concluded that both metals induced N- and Oglycosylation as a result of changed environmental conditions.

scholarly journals Genome-Wide Characterization and Analysis of Metallothionein Family Genes That Function in Metal Stress Tolerance in Brassica napus L.

2018 ◽  
Vol 19 (8) ◽  
pp. 2181 ◽  
Author(s):  
Yu Pan ◽  
Meichen Zhu ◽  
Shuxian Wang ◽  
Guoqiang Ma ◽  
Xiaohu Huang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Brassica Napus ◽  
Metal Binding ◽  
Genome Mapping ◽  
Expression Patterns ◽  
Biomass Productivity ◽  
Brassica Species ◽  
Brassica Napus L ◽  
Origin And Evolution ◽  
Duplication Events

Brassica plants exhibit both high biomass productivity and high rates of heavy metal absorption. Metallothionein (MT) proteins are low molecular weight, cysteine-rich, metal-binding proteins that play crucial roles in protecting plants from heavy metal toxicity. However, to date, MT proteins have not been systematically characterized in Brassica. In this study, we identified 60 MTs from Arabidopsis thaliana and five Brassica species. All the MT family genes from Brassica are closely related to Arabidopsis MTs, encoding putative proteins that share similar functions within the same clades. Genome mapping analysis revealed high levels of synteny throughout the genome due to whole genome duplication and segmental duplication events. We analyzed the expression levels of 16 Brassica napus MTs (BnaMTs) by RNA-sequencing and real-time RT-PCR (RT-qPCR) analysis in plants under As3+ stress. These genes exhibited different expression patterns in various tissues. Our results suggest that BnaMT3C plays a key role in the response to As3+ stress in B. napus. This study provides insight into the phylogeny, origin, and evolution of MT family members in Brassica, laying the foundation for further studies of the roles of MT proteins in these important crops.

Tobacco Chemistry/Chemische Zusammensetzung des Tabaks

1976 ◽  
Vol 8 (6) ◽  
Author(s):  
Arne J. Aasen ◽  
Sven-Olof Almquist ◽  
Curt R. Enzell
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L

Abstract35: two isomeric 5,6-Epoxy-3-hydroxy-7-megastigmen-9-ones from Nicotiana tabacum L.

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