Characterization and comparison of the soluble and membrane-bound cyclic AMP-dependent protein kinase from swine kidney

1983 ◽  
Vol 50 (2) ◽  
Author(s):  
Kalappagowda Muniyappa ◽  
FredrichH. Leibach ◽  
Joseph Mendicino
1975 ◽  
Vol 24 (1) ◽  
pp. 145-159 ◽  
Author(s):  
Rolf Kinne ◽  
Linda J. Shlatz ◽  
Evamaria Kinne-Saffran ◽  
Irving L. Schwartz

1986 ◽  
Vol 238 (2) ◽  
pp. 341-344 ◽  
Author(s):  
J G Church ◽  
J B Derdemezi ◽  
S Yuan ◽  
A K Sen

Highly purified sarcolemma from dog and pig cardiac muscle has been shown to contain significant activities of a membrane-bound cyclic AMP-dependent protein kinase. In addition, these membranes undergo endogenous phosphorylation when incubated with Mg2+ and [gamma-32P]ATP. By comparing 32P-labelled patterns obtained with [gamma-32P]ATP and the photoaffinity label 8-azidoadenosine 3′:5′-[32P]monophosphate (8-azido-cyclic [32P]AMP), we have demonstrated that, whereas the major kinase isoenzyme in dog sarcolemma was Type II, that in the pig membrane was the Type I isoenzyme.


1984 ◽  
Vol 259 (1) ◽  
pp. 654-661 ◽  
Author(s):  
I H Majerfeld ◽  
B H Leichtling ◽  
J A Meligeni ◽  
E Spitz ◽  
H V Rickenberg

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