Proliferative kinetics and mitomycin C-induced chromosome damage in Fanconi's anemia lymphocytes

1983 ◽  
Vol 63 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Kunihiko Miura ◽  
Kanehisa Morimoto ◽  
Akira Koizumi
Keyword(s):  
Mitomycin C ◽  
Chromosome Damage ◽  
Fanconi's Anemia ◽  
Fanconi’S Anemia

scholarly journals A test for Fanconi's anemia

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1637-1641
Author(s):  
J German ◽  
S Schonberg ◽  
S Caskie ◽  
D Warburton ◽  
C Falk ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Mitomycin C ◽  
Whole Blood ◽  
Normal Person ◽  
Diamond Blackfan Anemia ◽  
Fanconi's Anemia ◽  
Fanconi’S Anemia ◽  
Opposite Sex ◽  
Bona Fide ◽  
Normal Laboratory

A simple and reliable cytogenetic test for Fanconi's anemia (FA) that is based on the hypersensitivity of FA cells to mitomycin C (MC) is described. Equal volumes of whole blood from a patient in whom the diagnosis of FA is suspected and from a normal person of the opposite sex are co-cultured in phytohemagglutinin-containing medium in the presence and absence of MC. After five days' co-cultivation, 100 quinacrine-stained metaphases from both the MC-containing and the MC- free cultures are examined for the presence of a Y chromosome using fluorescence microscopy. In all bona fide FA patients in whom testing was successful, hypersensitivity to MC was readily demonstrated by the striking deficiency of FA metaphases (0.9% to 14.9%) in the MC- containing co-cultures. In contrast, none of the three patients with Diamond-Blackfan anemia and none of the five with undiagnosed conditions reminiscent of FA exhibited hypersensitivity to MC; cells from them, from parents of FA patients, and from several normal laboratory personnel constituted approximately half of the metaphases (40.4% to 71.2%) of MC-containing co-cultures, as would be expected in the absence of hypersensitivity to MC.

scholarly journals Flow cytometric characterization of the response of Fanconi's anemia cells to mitomycin C treatment

Cytometry ◽  
2005 ◽  
Vol 2 (5) ◽  
pp. 291-297 ◽  
Author(s):  
T. N. Kaiser ◽  
A. Lojewski ◽  
C. Dougherty ◽  
L. Juergens ◽  
E. Sahar ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Flow Cytometric ◽  
Fanconi's Anemia ◽  
Fanconi’S Anemia

scholarly journals A test for Fanconi's anemia

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1637-1641 ◽  
Author(s):  
J German ◽  
S Schonberg ◽  
S Caskie ◽  
D Warburton ◽  
C Falk ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Mitomycin C ◽  
Whole Blood ◽  
Normal Person ◽  
Diamond Blackfan Anemia ◽  
Fanconi's Anemia ◽  
Fanconi’S Anemia ◽  
Opposite Sex ◽  
Bona Fide ◽  
Normal Laboratory

Abstract A simple and reliable cytogenetic test for Fanconi's anemia (FA) that is based on the hypersensitivity of FA cells to mitomycin C (MC) is described. Equal volumes of whole blood from a patient in whom the diagnosis of FA is suspected and from a normal person of the opposite sex are co-cultured in phytohemagglutinin-containing medium in the presence and absence of MC. After five days' co-cultivation, 100 quinacrine-stained metaphases from both the MC-containing and the MC- free cultures are examined for the presence of a Y chromosome using fluorescence microscopy. In all bona fide FA patients in whom testing was successful, hypersensitivity to MC was readily demonstrated by the striking deficiency of FA metaphases (0.9% to 14.9%) in the MC- containing co-cultures. In contrast, none of the three patients with Diamond-Blackfan anemia and none of the five with undiagnosed conditions reminiscent of FA exhibited hypersensitivity to MC; cells from them, from parents of FA patients, and from several normal laboratory personnel constituted approximately half of the metaphases (40.4% to 71.2%) of MC-containing co-cultures, as would be expected in the absence of hypersensitivity to MC.

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