Integration of repeated sequences (pBR322) in the Bacillus subtilis 168 chromosome without affecting the genome structure

1993 ◽  
Vol 241-241 (3-4) ◽  
pp. 287-297 ◽  
Author(s):  
Mitsuhiro Itaya
Keyword(s):  
Bacillus Subtilis ◽  
Genome Structure ◽  
Repeated Sequences ◽  
Bacillus Subtilis 168

A new method for multiple gene inactivations in Bacillus subtilis 168, producing a strain free of selectable markers

2011 ◽  
Vol 57 (5) ◽  
pp. 427-436 ◽  
Author(s):  
Chong Zhang ◽  
Xiaohui Zhang ◽  
Zhengying Yao ◽  
Yaping Lu ◽  
Fengxia Lu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Resistance Gene ◽  
Target Genes ◽  
Genome Structure ◽  
Subtilis Strain ◽  
Gene Target ◽  
Selectable Markers ◽  
Vector Integration ◽  
Bacillus Subtilis 168 ◽  
Counter Selection

This study describes a novel method for repeated gene inactivation in Bacillus subtilis 168. A B. subtilis strain (BS-PS) that is conditionally auxotrophic for lysine was obtained by replacing the PlysA promoter with the Pspac promoter. The homologous recombination integration vector PLC-T was constructed to contain lacI, which encodes a Pspac promoter repressor, and the chloromycetin resistance gene. Target genes were manipulated by generating an insertion sequence with two homologous arms and the target gene in PLC-T to create a specific integrating vector. Integration into the BS-PS chromosome occurred by a single crossover at either of the two homologous arms. The resulting transitional strain (BS-PS-PI) was chloromycetin resistant and lysine auxotrophic and had an unstable genome structure because of the duplication. Excision of lacI and chloromycetin resistance gene was achieved by a second single crossover at the duplication. Recovery of a lysine prototroph functioned as counter-selection and was identified by PCR. In this work, we inactivated nprE and aprE, two protease genes secreted by B. subtilis 168 free of selectable markers.

Crystal Structure of Urate Oxidase from Bacillus Subtilis 168

2019 ◽  
Vol 64 (7) ◽  
pp. 1126-1133 ◽  
Author(s):  
A. Nayab ◽  
S. A. Moududee ◽  
Y. Shi ◽  
Y. Jiang ◽  
Q. Gong
Keyword(s):  
Crystal Structure ◽  
Bacillus Subtilis ◽  
Urate Oxidase ◽  
Bacillus Subtilis 168

In vitro characterization of N-terminal truncated EpsC from Bacillus subtilis 168, a UDP-N-acetylglucosamine 4,6-dehydratase

2018 ◽  
Vol 657 ◽  
pp. 78-88 ◽  
Author(s):  
Chinmayi R. Kaundinya ◽  
Handanahal S. Savithri ◽  
K. Krishnamurthy Rao ◽  
Petety V. Balaji
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Subtilis 168 ◽  
In Vitro Characterization

An SfiI restriction map of the Bacillus subtilis 168 genome

Gene ◽  
1991 ◽  
Vol 101 (1) ◽  
pp. 15-21 ◽  
Author(s):  
M. Amjad ◽  
J.M. Castro ◽  
H. Sandoval ◽  
J.-J. Wu ◽  
M. Yang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Restriction Map ◽  
Bacillus Subtilis 168
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