Cell culture studies on neurofibromatosis (von Recklinghausen). II. Occurrence of glial cells in primary cultures of peripheral neurofibromas

1983 ◽  
Vol 63 (3) ◽  
Author(s):  
W. Krone ◽  
G. Jirikowski ◽  
O. M�hleck ◽  
H. Kling ◽  
H. Gall
Keyword(s):  
Cell Culture ◽  
Glial Cells ◽  
Primary Cultures ◽  
Culture Studies ◽  
Von Recklinghausen

Cell culture studies on neurofibromatosis (von Recklinghausen)

1986 ◽  
Vol 74 (4) ◽  
pp. 453-455 ◽  
Author(s):  
Winfrid Krone ◽  
Inge H�gemann
Keyword(s):  
Cell Culture ◽  
Culture Studies ◽  
Von Recklinghausen

Cell Culture Studies on Neurofibromatosis (von Recklinghausen).

1986 ◽  
Vol 486 (1 Neurofibromat) ◽  
pp. 354-370 ◽  
Author(s):  
W. KRONE ◽  
R. MAO ◽  
O. S. MÜHLECK ◽  
H. KLING ◽  
T. FINK
Keyword(s):  
Cell Culture ◽  
Culture Studies ◽  
Von Recklinghausen

The actions of leptin on survival and hydrogen peroxide toxicity in primary mixed glial cells of rat

Biologia ◽  
2007 ◽  
Vol 62 (6) ◽  
Author(s):  
Selda Kabadere ◽  
Gokhan Kus ◽  
Ruhi Uyar ◽  
Pinar Oztopcu-Vatan ◽  
Nilufer Erkasap ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Glial Cell ◽  
Glial Cells ◽  
Cell Types ◽  
Positive Control ◽  
Culture Studies ◽  
Mtt Method ◽  
Diphenyltetrazolium Bromide

AbstractStudies indicate that leptin is involved in not only energy expenditure and food intake, but also in protection against apoptosis, in inflammation and in stimulation of proliferation in many cell types. However, leptin treatment increases the oxidative stress in many cell culture studies. This contradiction evoked a question of whether leptin acts as an oxidant or antioxidant on glial cells. We investigated the effect of leptin on glial cell survival and hydrogen peroxide (H2O2)-induced toxicity in vitro. The survival rate of the cells was determined by using 3-(4,5-D-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, thyazolyl blue (MTT) method. The cells obtained from the whole brain of 1–3 day-old rat were treated with 1, 10, 100 and 1000 ng/mL leptin for 24 or 72 h. Either the pretreatment of leptin alone for 5 h or leptin combined simultaneously with H2O2 or well known antioxidant glutathione (GSH) were applied to the cells. Malondialdehyde (MDA) levels were measured in cell lysates to which leptin was added for 24 h. The 100 and 1000 ng/mL leptin treatment for 72 h increased the glial viability by 19% and 36%, respectively. The dose of H2O2 that killed 75% of the cells was determined as 100 µM. GSH at different doses was applied as a positive control to the cells and the dose of 500 µM completely eliminated toxic effect of 100 µM H2O2. Either the pretreatment of leptin alone for 5 h or leptin combined simultaneously with H2O2 could not eliminate H2O2-caused toxicity. Furthermore, respective leptin doses did not change the glia MDA level. We suggest that leptin can increase glia survival dose dependently, but can not eliminate H2O2-induced oxidation in primary mixed glial cell culture.

Cell culture studies on neurofibromatosis (von Recklinghausen)

1981 ◽  
Vol 58 (2) ◽  
pp. 188-193 ◽  
Author(s):  
Winfrid Krone ◽  
Sybille Z�rlein ◽  
Richard Mao
Keyword(s):  
Cell Culture ◽  
Culture Studies ◽  
Von Recklinghausen

scholarly journals Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions

2021 ◽  
Author(s):  
Mridul Johari ◽  
Jaakko Sarparanta ◽  
Anna Vihola ◽  
Per Harald Jonson ◽  
Marco Savarese ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput Sequencing ◽  
Muscle Protein ◽  
Distal Myopathy ◽  
Missense Mutations ◽  
Limb Muscle ◽  
Muscle Involvement ◽  
Culture Studies ◽  
Protein X ◽  
Small Muscle

AbstractUsing deep phenotyping and high-throughput sequencing, we have identified a novel type of distal myopathy caused by mutations in the Small muscle protein X-linked (SMPX) gene. Four different missense mutations were identified in ten patients from nine families in five different countries, suggesting that this disease could be prevalent in other populations as well. Haplotype analysis of patients with similar ancestry revealed two different founder mutations in Southern Europe and France, indicating that the prevalence in these populations may be higher. In our study all patients presented with highly similar clinical features: adult-onset, usually distal more than proximal limb muscle weakness, slowly progressing over decades with preserved walking. Lower limb muscle imaging showed a characteristic pattern of muscle involvement and fatty degeneration. Histopathological and electron microscopic analysis of patient muscle biopsies revealed myopathic findings with rimmed vacuoles and the presence of sarcoplasmic inclusions, some with amyloid-like characteristics. In silico predictions and subsequent cell culture studies showed that the missense mutations increase aggregation propensity of the SMPX protein. In cell culture studies, overexpressed SMPX localized to stress granules and slowed down their clearance.

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

Electrospun poly (N-isopropylacrylamide-co-acrylic acid)/cellulose laurate blend nanofibers containing adapalene: Morphology, drug release, and cell culture studies

2016 ◽  
Vol 65 (9) ◽  
pp. 477-486 ◽  
Author(s):  
Morteza Ziaee ◽  
Payam Zahedi ◽  
Majid Abdouss ◽  
Mohammad Amin Zarandi ◽  
Saeed Manouchehri ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Release ◽  
Acrylic Acid ◽  
Culture Studies
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