Chromosomal location by in situ hybridization of the human Sau3A family of DNA repeats

1987 ◽  
Vol 75 (4) ◽  
pp. 326-332 ◽  
Author(s):  
Alessandra Agresti ◽  
G. Rainaldi ◽  
Andrea Lobbiani ◽  
Ivana Magnani ◽  
R. Di Lernia ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Location ◽  
Dna Repeats

The genomic composition of Tricepiro, a synthetic forage crop

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 154-159 ◽  
Author(s):  
María Rosa Ferrari ◽  
Eduardo J Greizerstein ◽  
Héctor A Paccapelo ◽  
Carlos A Naranjo ◽  
Angelines Cuadrado ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Location ◽  
Hexaploid Triticale ◽  
Genomic Constitution ◽  
Forage Crop ◽  
A Genome ◽  
Total Dna ◽  
Synthetic Hexaploid ◽  
Specific Sequences

Chromosome in situ hybridization (FISH and GISH) is a powerful tool for determining the chromosomal location of specific sequences and for analysing genome organization and evolution. Tricepiro (2n = 6x = 42) is a synthetic cereal obtained by G. Covas in Argentina (1972), which crosses hexaploid triticale (2n = 6x = 42) and octoploid Trigopiro (2n = 8x = 56). Several years of breeding produced a forage crop with valuable characteristics from Secale, Triticum, and Thinopyrum. The aim of this work is to analyse the real genomic constitution of this important synthetic crop. In situ hybridization using total DNA of Secale, Triticum, and Thinopyrum as a probe (GISH) labelled with biotin and (or) digoxigenin showed that tricepiro is composed of 14 rye chromosomes and 28 wheat chromosomes. Small zones of introgression of Thinopyrum on wheat chromosomes were detected. The FISH using the rye repetitive DNA probe pSc 119.2 labelled with biotin let us characterize the seven pairs of rye chromosomes. Moreover, several wheat chromosomes belonging to A and B genomes were distinguished. Therefore, tricepiro is a synthetic hexaploid (2n = 6x = 42) being AABBRR in its genomic composition, with zones of introgression of Thinopyrum in the A genome of wheat.Key words: tricepiro, trihybrid, Triticum, Secale, Thinopyrum, in situ hybridization, FISH, GISH, genomic composition, synthetic forage crop.

scholarly journals Applications of fluorescence in situ hybridization (FISH) in genome research

2020 ◽  
Vol 17 (3) ◽  
pp. 393-410
Author(s):  
Hoang Thi Nhu Phuong ◽  
Huynh Thi Thu Hue ◽  
Cao Xuan Hieu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequences ◽  
Dna Probes ◽  
Dna Repeats ◽  
Genome Research ◽  
Multicolor Fish ◽  
Vector Systems ◽  
Assembly Sequences

Fluorescence in situ hybridization (FISH) technique enables the direct detection of DNA sequences inintact cellular materials (e.g. individual chromosomes in metaphase spreads). This review article focuses on theapplications of FISH in genome research, including validation and correction of the genome assembly from thenext-generation sequencing (NGS) projects. DNA probes for specific DNA fragments of the assembly can beobtained from PCR amplicon or cloned products using different vector systems. Localization of these probeson their respective chromosomal regions can be visualized by FISH, providing useful information to crosscheckthe assembly data. Furthermore, the recent refinements in the FISH technology including using smartpooling scheme of differently colored DNA probes, together with consecutive FISH experiments (stripping andreprobing of the same slide) are described. These advances in multicolor FISH can provide crucial linkageinformation on association of linkage groups and assembly scaffolds, resulting in so-called cytogenetic maps.Integration of the cytogenetic maps and assembly sequences assists to resolve the chromosome-level genomeassembly and to reveal new insights in genome architecture and genome evolution. Especially, comparativechromosome painting with pooled DNA probes from one reference species can be used to investigate ancestralrelationships (chromosome homeology and rearrangements) among other not-yet-sequenced species. Inaddition, FISH using DNA probes for certain specific classes of repetitive DNA elements as well as for basicchromosome structures (e.g. centromere or telomere DNA repeats, ribosomal DNA loci) can be used to studythe genome organization and karyotype differentiation. We also discussed about limitations and futureperspectives of the FISH technology.

Integration of growth hormone gene constructs in transgenic strains of coho salmon (Oncorhynchus kisutch) at centromeric or telomeric sites

Genome ◽  
2010 ◽  
Vol 53 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Ruth B. Phillips ◽  
Robert H. Devlin
Keyword(s):  
Growth Hormone ◽  
Coho Salmon ◽  
Chromosomal Location ◽  
Genetically Engineered ◽  
Growth Hormone Gene ◽  
Dna Repeats ◽  
Homologous Chromosomes ◽  
Chromosome Arm ◽  
Different Strains

Very little information is currently available regarding the sites of integration of transgenes in genetically engineered fish. We examined the chromosomal location of growth hormone gene constructs containing GH1 in three different strains of transgenic coho salmon produced by microinjection into pronuclei of fertilized eggs. The constructs were labeled and used as probes in fluorescence in situ hybridization experiments on chromosome preparations from the M77, MT5750A, and H3D0474 strains of transgenic coho salmon. The constructs were localized at 1–3 different sites in different strains. In the M77 strain the construct was found at a single centromeric site on a medium-sized metacentric chromosome, while in the MT5750A strain, the construct was found at a single telomeric site on the short arm of chromosome pair 21, a subtelocentric chromosome with a large band of repetitive DNA on the short arm. In the H3D0474 strain, the construct was found at telomeric sites on the long arms of three metacentric chromosomes that appear to represent one pair of homologous chromosomes and one chromosome containing the homeologous long arm (recently duplicated chromosome arm) corresponding to the long arm of the first pair. This suggests transfer of the construct may have occurred by homologous and homeologous crossing over. All of the constructs incorporated at restricted sites characterized by the presence of tandem DNA repeats.

Chromosomal location of rRNA genes in Diprion pini detected by in situ hybridization

1999 ◽  
Vol 322 (6) ◽  
pp. 461-466 ◽  
Author(s):  
Jérôme Rousselet ◽  
Nicole Chaminade ◽  
Claude Géri ◽  
Godfrey M. Hewitt ◽  
Françoise Lemeunier
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Location ◽  
Rrna Genes ◽  
Diprion Pini

scholarly journals Mapping of the nu gene using congenic nude strains and in situ hybridization.

1992 ◽  
Vol 175 (3) ◽  
pp. 873-876 ◽  
Author(s):  
Y Takahashi ◽  
A Shimizu ◽  
T Sakai ◽  
Y Endo ◽  
N Osawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Mouse Chromosome ◽  
Nude Mouse ◽  
Physical Mapping ◽  
Dna Markers ◽  
Chromosomal Location ◽  
Chromosome 11 ◽  
Mouse Chromosome 11

The chromosomal location of the nu gene, which is responsible for hairlessness and athymus, was determined using six DNA markers (interleukin 3 [Il-3], Myhs, Acrb, Evi-2, Mpo, and Hox-2) on mouse chromosome 11. We constructed the high-resolution physical mapping of the six DNA markers on chromosome 11 by in situ hybridization using fluorescence-labeled cosmid probes. The results indicate the order of centromere-(41cM)-Il-3-(3cM)-Myhs- (4cM)-Acrb-(6cM)-Evi-2-(3cM)-Mpo-(5cM)- Hox-2. We have used congenic nude strains and examined which of the six DNA markers were derived from the original nude mouse. We found the Evi-2 locus is linked to the nu gene in all the informative, independent congenic nude strains. From these data, we could estimate the location of the nu gene, not only genetically but also physically within a region that spans approximately 17 megabases (9 cM) between the Acrb and Mpo genes.

scholarly journals On the Genomic Location of theexuperantia1Gene inDrosophila miranda: The Limits ofin SituHybridization Experiments

Genetics ◽  
2003 ◽  
Vol 164 (3) ◽  
pp. 1237-1240 ◽  
Author(s):  
Doris Bachtrog ◽  
Brian Charlesworth
Keyword(s):  
In Situ Hybridization ◽  
X Chromosome ◽  
Sex Chromosome ◽  
Chromosomal Location ◽  
Polytene Chromosomes ◽  
Gene Families ◽  
Hybridization Signal ◽  
Genomic Location ◽  
Multiple Copies

AbstractIn situ hybridization to Drosophila polytene chromosomes is a powerful tool for determining the chromosomal location of genes. Using in situ hybridization experiments, Yi and Charlesworth recently reported the transposition of the exuperantia1 gene (exu1) from a neo-sex chromosome to the ancestral X chromosome of Drosophila miranda, close to exuperantia2 (exu2). By characterizing sequences flanking exu1, however, we found the position of exu1 to be conserved on the neo-sex chromosome. Further, the exu2 gene was found to be tandemly duplicated on the X chromosome of D. miranda. The misleading hybridization signal of exu1 may be caused by multiple copies of exu2, which interfere with the hybridization of the exu1 probe to its genomic position on the neo-X chromosome. This suggests that flanking DNA should be used to confirm the positions of members of gene families.

Definitive chromosomal location of the H-2 complex by in situ hybridization to pachytene chromosomes

1985 ◽  
Vol 22 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Eric Lader ◽  
Brian T. Clark ◽  
S. C. Jhanwar ◽  
R. S. K. Chaganti ◽  
Dorothea Bennett
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Location

scholarly journals Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae)

2019 ◽  
Author(s):  
Alexandra V. Amosova ◽  
Svyatoslav A. Zoshchuk ◽  
Alexander V. Rodionov ◽  
Lilit Ghukasyan ◽  
Tatiana E. Samatadze ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Molecular Cytogenetics ◽  
5S Rdna ◽  
Arctic Tundra ◽  
The Arctic ◽  
Grass Species ◽  
Dna Repeats ◽  
Microsatellite Motif ◽  
Pasture Grass

Abstract Background Grasslands in the Arctic tundra undergo irreversible degradation due to climatic changes and also over-exploitation and depletion of scarce resources. Comprehensive investigations of cytogenomic structures of valuable Arctic and sub-Arctic grassland species is essential for clarifying their genetic peculiarities and phylogenetic relationships and also successful developing new forage grass cultivars with high levels of adaptation, stable productivity and longevity. We performed molecular cytogenetic characterization of seven insufficiently studied pasture grass species from related genera Alopecurus, Arctagrostis, Beckmannia, Deschampsia and Holcus (Poaceae) which are the primary fodder resources in the Arctic tundra. Results For these species, integrated schematic habitat maps were constructed based on the available data on their distribution in Eurasia. The species karyotypes were examined with the use of DAPI-banding, fluorescence in situ hybridization with 35S rDNA, 5S rDNA and the (GTT)9 microsatellite motif and also sequential rapid genomic in situ hybridization with genomic DNAs of Deschampsia sukatschewii, Holcus lanatus and Deschampsia flexuosa. Cytogenomic structures of the studied species were specified; peculiarities and common features of their genomes were revealed. Different chromosomal rearrangements were detected in Beckmannia syzigachne, Deschampsia cespitosa and D. flexuosa; B chromosomes with distinct DAPI-bands were observed in karyotypes of D. cespitosa and H. lanatus. Conclusions The peculiarities of distribution patterns of the examined chromosomal markers and also presence of common homologous DNA repeats in karyotypes of the studies species allowed us to verify their relationships. The obtained unique data on distribution areas and cytogenomic structures of the valuable Arctic and sub-Arctic pasture species are important for further genetic and biotechnological studies and also plant breeding progress.

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