S allele discrimination in styles of Petunia hybrida bearing stylar-conditioned pseudo-self-compatibility

1979 ◽  
Vol 55 (1) ◽  
pp. 23-28 ◽  
Author(s):  
D. R. Flaschenriem ◽  
P. D. Ascher
Keyword(s):  
Petunia Hybrida ◽  
Allele Discrimination ◽  
Self Compatibility ◽  
S Allele

scholarly journals How does the S-locus determining self-incompatibility in stone fruits work in self-compatible peach?

2005 ◽  
pp. 93-100
Author(s):  
Attila Hegedűs ◽  
Júlia Halász ◽  
Zoltán Szabó ◽  
József Nyéki ◽  
Andrzej Pedryc
Keyword(s):  
Prunus Persica ◽  
Fruit Trees ◽  
S Locus ◽  
Recognition Mechanism ◽  
Specific Pcr ◽  
Fruit Species ◽  
Self Fertilization ◽  
Self Compatibility ◽  
S Allele ◽  
Available Information

The majority of stone fruit species are self-incompatible, a feature that is determined by a specific recognition mechanism between the S-ribonuclease enzymes residing in the pistils and the F-box proteins expressed in the pollen tubes. Failure in the function of any component of this bipartite system resulted in self-compatibility (SC) in many cultivars of Prunus species. Peach (Prunus persica (L.) Batsch.) is the only species in the Prunoideae subfamily that is traditionally known to be self-compatible, but its molecular background is completely unknown. Isoelectric focusing and S-gene specific PCR revealed that SC is not due to functional inability of pistil ribonucleases. We hypothesize that SC may be a consequence of a kind of pollen-part mutation or the action of one or more currently unknown modifier gene(s). Only two S-alleles were identified in a set of peach genotypes of various origin and phenotypes in contrast to the 17–30 alleles described in self-incompatible fruit trees. Most important commercial cultivars carry the same S-allele and are in a homozygote state. This indicates the common origin of these cultivars and also the consequence of self-fertilization. According to the available information, this is the first report to elucidate the role of S-locus in the fertilization process of peach. 

scholarly journals Evaluation of Self-Incompatibility Locus Diversity of Domestic Plum (Prunus Domestica L.) using DNA-Based S-Genotyping

2013 ◽  
Vol 67 (2) ◽  
pp. 109-115 ◽  
Author(s):  
Irita Kota-Dombrovska ◽  
Gunārs Lācis
Keyword(s):  
Self Incompatibility ◽  
Limited Information ◽  
Prunus Domestica ◽  
Prunus Species ◽  
Allele Specific ◽  
Self Compatibility ◽  
S Allele ◽  
Functional Relevance ◽  
Further Development ◽  
Incompatibility Locus

There is limited information on genetics of self-incompatibility in domestic plum (P. domestica L.). In comparison with other Prunus species, there are no S-allele specific markers for the identification of compatibility groups, and thus, genetic diversity of self-compatibility genes is not clear. Six S-locus-specific markers previously used for other Prunus species were used to study genetics of self-compatibility of plums, and 33 domestic plum cultivars were genotyped. The applied primer pairs showed good transferability among Prunus species and showed high diversity in the tested plant material (14-37 alleles per marker, average observed heterozygocity - 0.953). Applification of the tested primer pairs allowed discrimination of all plum cultivars by unique S-genotypes. Based on the obtained results, primer pairs EM-PC2consFD/ EM-PC3cons RD, PasPcons-F1/ PaC1cons-R1 and F-Box50A/ F-Box intronR are suggested as supplementary markers for characterisation and identification of plum germplasm with potential functional relevance. Although the markers used did not strictly distinguish plum cultivars according to compatibility groups (selfcompatible, partly self-compatible and self-incompatible), group-unique amplification fragments were identified, which can serve as a baseline in further development of specific markers.

Discriminating styles (DS) and pollen-mediated pseudo-self compatibility (PMPSC) in Petunia hybrida hort

Euphytica ◽  
1985 ◽  
Vol 34 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Michael N. Dana ◽  
Peter D. Ascher
Keyword(s):  
Petunia Hybrida ◽  
Self Compatibility

scholarly journals Self-compatibility of 3 Apple Cultivars and Identification of S-allele Genotypes in Their Self-pollinated Progenies

2007 ◽  
Vol 6 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Akira Saito ◽  
Tomoko Fukasawa-Akada ◽  
Megumi Igarashi ◽  
Takashi Sato ◽  
Masahiko Suzuki
Keyword(s):  
Self Compatibility ◽  
Apple Cultivars ◽  
S Allele

Segregation Distortion of T-DNA Markers Linked to the Self-Incompatibility (S) Locus in Petunia hybrida

Genetics ◽  
2000 ◽  
Vol 154 (3) ◽  
pp. 1323-1333
Author(s):  
Robin M Harbord ◽  
Carolyn A Napoli ◽  
Timothy P Robbins
Keyword(s):  
Segregation Distortion ◽  
Petunia Hybrida ◽  
Selectable Marker ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus ◽  
Differential Transmission ◽  
S Allele ◽  
Maize Transposable Element ◽  
General Method

Abstract In plants with a gametophytic self-incompatibility system the specificity of the pollen is determined by the haploid genotype at the self-incompatibility (S) locus. In certain crosses this can lead to the exclusion of half the gametes from the male parent carrying a particular S-allele. This leads to pronounced segregation distortion for any genetic markers that are linked to the S-locus. We have used this approach to identify T-DNA insertions carrying a maize transposable element that are linked to the S-locus of Petunia hybrida. A total of 83 T-DNA insertions were tested for segregation distortion of the selectable marker used during transformation with Agrobacterium. Segregation distortion was observed for 12 T-DNA insertions and at least 8 of these were shown to be in the same linkage group by intercrossing. This indicates that differential transmission of a single locus (S) is probably responsible for all of these examples of T-DNA segregation distortion. The identification of selectable markers in coupling with a functional S-allele will allow the preselection of recombination events around the S-locus in petunia. Our approach provides a general method for identifying transgenes that are linked to gametophytic self-incompatibility loci and provides an opportunity for transposon tagging of the petunia S-locus.

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