Gene disruption in Lactobacillus plantarum strain 80 by site-specific recombination: isolation of a mutant strain deficient in conjugated bile salt hydrolase activity

1993 ◽  
Vol 239 (1-2) ◽  
pp. 269-272 ◽  
Author(s):  
R. J. Leer ◽  
H. Christiaens ◽  
W. Verstraete ◽  
L. Peters ◽  
M. Posno ◽  
...  
Keyword(s):  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Mutant Strain ◽  
Gene Disruption ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Bile Salt Hydrolase Activity

scholarly journals Bile salt hydrolase and antimicrobial activities of three bile resistant probiotics Lactobacillus plantarum strains isolated from Cameroonian artisanal fermented milk

2017 ◽  
Vol 7 (5) ◽  
pp. 21
Author(s):  
Bemmo Kamdem Ulrich Landry ◽  
Kaktcham Pierre-Marie ◽  
Momo Kenfack Chancel Hector ◽  
Foko Kouam Edith Marius ◽  
ZAMBOU NGOUFACK FRANCOIS ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Fermented Milk ◽  
Antimicrobial Activities ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Bile Tolerance ◽  
Spoilage Bacteria ◽  
Bile Salt Hydrolase Activity

Probiotics are well known for their efficacy as dietary adjuncts providing benefits to consumers. However, the selection of probiotics before incorporation into diet requires the scrutiny of a well-defined set of criteria. Hence, three Lactobacillus plantarum strains (GGU, GLA51 and GLP56, having KU949009, KU949010 and KU949011 respectively as GenBank accession numbers) previously isolated from Cameroonian traditional fermented milk have been used in this study for the evaluation of their Bile salt hydrolase and antimicrobial activities. Bile tolerance test was carried out by monitoring the bacterial growth at different Oxgall bile concentration (0.3%, 0.5% and 1%). The bile salt hydrolase activity was measured by determining the amount of amino acid liberated from conjugated bile salts by lactobacilli strains. Also, three bshgenes (bsh1 gene specific to Lactobacillus plantarum species, bshA and bshB genes specific to Lactobacillus acidophilus species) involved in the bile salt hydrolase activity were screened. The antimicrobial activity of the strains was evaluated using the agar-spot test. The three Lactobacillus plantarum strains showed survival percentages higher than 90% in the presence of 0.3% of Oxgall bile, and the delays of growth ranged from 0 to 10 min. Their bile salt hydrolase activity ranged from 15.62 ± 3.00 to 23.91 ± 5.82 U/mg towards Oxgall and from 10.47 ± 2.76 to 24.57 ± 6.31 U/mg towards Taurodeoxycholate. Only Lactobacillus plantarum GLA51 was found to harbor thebsh1 gene. Its sequence was deposited in the Genbank under the accession number MF098542. The analysis of thisbsh1 gene sequence from L. plantarum GLA51 indicated that it contained an open reading frame (ORF) of 838 nucleotides encoding a 278 amino acids protein. The three Lactobacilli strains showed inhibitory activity against the pathogenic or spoilage bacteria tested, except for Shigella flexneri. These results suggest that the three Lactobacillus plantarum strain show potential for probiotic applications.Keywords: Bile salt hydrolase activity, antimicrobial activity, Lactobacillus plantarum, bile tolerance 

Relationship between bile salt hydrolase activity, changes in the internal pH and tolerance to bile acids in lactic acid bacteria

2012 ◽  
Vol 34 (8) ◽  
pp. 1511-1518 ◽  
Author(s):  
Ana Yanina Bustos ◽  
Lucila Saavedra ◽  
Graciela Font de Valdez ◽  
Raúl Ricardo Raya ◽  
María Pía Taranto
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Bile Acids ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Internal Ph ◽  
Bile Salt Hydrolase Activity

Bile tolerance and bile salt hydrolase activity of lactobacilli and bifidobacteria isolated from the human intestine

1995 ◽  
Vol 108 (4) ◽  
pp. A928
Author(s):  
G. Thornton ◽  
M. O'Sullivan ◽  
G. O'Sullivan ◽  
A. Weerkamp ◽  
F. Shanahan ◽  
...  
Keyword(s):  
Bile Salt ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Human Intestine ◽  
Bile Tolerance ◽  
Bile Salt Hydrolase Activity
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