Barley embryo globulin 1 gene, Beg1: Characterization of cDNA, chromosome mapping and regulation of expression

1993 ◽  
Vol 239 (1-2) ◽  
pp. 209-218 ◽  
Author(s):  
Gregory R. Heck ◽  
Aaron K. Chamberlain ◽  
T.-H. David Ho
Keyword(s):  
Chromosome Mapping ◽  
Regulation Of Expression ◽  
Barley Embryo

scholarly journals Chromosome Mapping of Repetitive Sequences inRachycentron canadum(Perciformes: Rachycentridae): Implications for Karyotypic Evolution and Perspectives for Biotechnological Uses

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Uedson Pereira Jacobina ◽  
Marcelo de Bello Cioffi ◽  
Luiz Gustavo Rodrigues Souza ◽  
Leonardo Luiz Calado ◽  
Manoel Tavares ◽  
...  
Keyword(s):  
Repetitive Sequences ◽  
Chromosome Mapping ◽  
Karyotypic Evolution ◽  
Dapi Staining ◽  
Telomeric Sequences ◽  
Replication Banding ◽  
The Family ◽  
Early Replication

The cobia,Rachycentron canadum, a species of marine fish, has been increasingly used in aquaculture worldwide. It is the only member of the family Rachycentridae (Perciformes) showing wide geographic distribution and phylogenetic patterns still not fully understood. In this study, the species was cytogenetically analyzed by different methodologies, including Ag-NOR and chromomycin A3(CMA3)/DAPI staining, C-banding, early replication banding (RGB), andin situfluorescent hybridization with probes for 18S and 5S ribosomal genes and for telomeric sequences (TTAGGG)n. The results obtained allow a detailed chromosomal characterization of the Atlantic population. The chromosome diversification found in the karyotype of the cobia is apparently related to pericentric inversions, the main mechanism associated to the karyotypic evolution of Perciformes. The differential heterochromatin replication patterns found were in part associated to functional genes. Despite maintaining conservative chromosomal characteristics in relation to the basal pattern established for Perciformes, some chromosome pairs in the analyzed population exhibit markers that may be important for cytotaxonomic, population, and biodiversity studies as well as for monitoring the species in question.

scholarly journals Characterization of Four Lectin-Like Receptor Kinases Expressed in Roots of Medicago truncatula. Structure, Location, Regulation of Expression, and Potential Role in the Symbiosis with Sinorhizobium meliloti

2003 ◽  
Vol 133 (4) ◽  
pp. 1893-1910 ◽  
Author(s):  
Maria-Téresa Navarro-Gochicoa ◽  
Sylvie Camut ◽  
Antonius C.J. Timmers ◽  
Andreas Niebel ◽  
Christine Hervé ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Sinorhizobium Meliloti ◽  
Potential Role ◽  
Regulation Of Expression ◽  
Receptor Kinases

scholarly journals Diacylglycerol Kinase Is Involved in Regulation of Expression of the Lantibiotic Mutacin II of Streptococcus mutans

1998 ◽  
Vol 180 (1) ◽  
pp. 167-170 ◽  
Author(s):  
Ping Chen ◽  
Jan Novak ◽  
Fengxia Qi ◽  
Page W. Caufield
Keyword(s):  
Environmental Stress ◽  
Gene Product ◽  
Streptococcus Mutans ◽  
Structural Gene ◽  
Genetic Characterization ◽  
Diacylglycerol Kinase ◽  
Primer Extension ◽  
Regulation Of Expression ◽  
Integration Vector

ABSTRACT Genetic characterization of a Tn916 transposon mutant,Streptococcus mutans T8-1, defective in mutacin II production, revealed that the transposon was inserted into the 3′ region of a diacylglycerol kinase (dgk) gene. The insertion occurred in the same region as described for another S. mutans mutant, GS5Tn1, which was altered in its ability to respond to environmental stress (Y. Yamashita, T. Takehara, and H. K. Kuramitsu, J. Bacteriol. 175:6220–6228, 1993). Quantitative primer extension from the mutacin structural gene mutA showed a decreased level (about eightfold) of mutA transcription for mutant T8-1. Mutacin production was restored by transforming mutant T8-1 with integration vector pVA891 containing an intactdgk gene. These data indicated that the full-lengthdgk gene product along with the mutacin biosynthetic operon are required for the production of the mutacin II lantibiotic.

scholarly journals Further characterization of erythrocyte p80 and the membrane protein defect of In(Lu) Lu(a-b-) erythrocytes

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1475-1481
Author(s):  
MJ Telen ◽  
I Rogers ◽  
M Letarte
Keyword(s):  
Monoclonal Antibody ◽  
Membrane Protein ◽  
Concanavalin A ◽  
Polyclonal Antibodies ◽  
Erythrocyte Ghosts ◽  
Con A ◽  
Down Regulation ◽  
Regulation Of Expression ◽  
Variable Effect

We have previously shown that the In(Lu) gene down-regulates expression of an erythrocyte protein antigen identified by murine monoclonal antibody (MoAb) A3D8. In the present study we have examined In(Lu) Lu(a- b-) erythrocytes for expression of additional epitopes on the erythrocyte 80 kilodalton protein (p80) bearing the A3D8 antigen. Using a total of seven additional MoAbs that recognize three epitopes on erythrocyte p80, we have shown that In(Lu) Lu(a-b-) erythrocytes exhibit down-regulation of expression of all three epitopes. In(Lu) erythrocytes also showed a reduction in their reactivity to rabbit antibodies produced against purified p80 from either erythrocytes or lymphocytes. Furthermore the reactivity of the MoAbs was not altered by treatment of the cells with neuraminidase but was substantially reduced by treatment of cells with trypsin or chymotrypsin. The polyclonal anti- p80 sera were shown to react with a fragment of 50,000 daltons, still associated with erythrocyte ghosts, following treatment of the cells with trypsin or chymotrypsin. Treatment of erythrocytes with the thiol- reactive reagent AET decreased their reactivity with the MoAbs but had a variable effect on their reactivity with polyclonal antibodies. Erythrocyte p80 is a glycoprotein with N-linked oligosaccharides, as demonstrated by its binding to concanavalin A (Con A) and Len culinaris lectins. Following Endoglycosidase F treatment, erythrocyte p80 underwent a reduction in apparent mol wt of 11,000. The presence of a reduced amount of an intact p80 glycoprotein, seen by a decrease in reactivity with MoAbs directed at three distinct epitopes and with two different polyclonal antibodies, suggests that the In(Lu) gene interferes with expression by erythrocytes of the entire p80 glycoprotein.

scholarly journals Bioinformatics as a Tool for the Structural and Evolutionary Analysis of Proteins

2020 ◽  
Author(s):  
Edna María Hernández-Domínguez ◽  
Laura Sofía Castillo-Ortega ◽  
Yarely García-Esquivel ◽  
Virginia Mandujano-González ◽  
Gerardo Díaz-Godínez ◽  
...  
Keyword(s):  
Biological Molecules ◽  
Evolutionary Analysis ◽  
Evolutionary Processes ◽  
Regulation Of Expression ◽  
Computational Mathematics ◽  
Functional Changes ◽  
Sequencing Technologies ◽  
Metabolomic Data ◽  
Changes Over Time

This chapter deals with the topic of bioinformatics, computational, mathematics, and statistics tools applied to biology, essential for the analysis and characterization of biological molecules, in particular proteins, which play an important role in all cellular and evolutionary processes of the organisms. In recent decades, with the next generation sequencing technologies and bioinformatics, it has facilitated the collection and analysis of a large amount of genomic, transcriptomic, proteomic, and metabolomic data from different organisms that have allowed predictions on the regulation of expression, transcription, translation, structure, and mechanisms of action of proteins as well as homology, mutations, and evolutionary processes that generate structural and functional changes over time. Although the information in the databases is greater every day, all bioinformatics tools continue to be constantly modified to improve performance that leads to more accurate predictions regarding protein functionality, which is why bioinformatics research remains a great challenge.

scholarly journals Characterization of Streptococcus pneumoniae TrmD, a tRNA Methyltransferase Essential for Growth

2004 ◽  
Vol 186 (8) ◽  
pp. 2346-2354 ◽  
Author(s):  
Karen O'Dwyer ◽  
Joseph M. Watts ◽  
Sanjoy Biswas ◽  
Jennifer Ambrad ◽  
Michael Barber ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Kinetic Studies ◽  
Mobility Shift ◽  
Regulation Of Expression ◽  
E Coli ◽  
Trna Species ◽  
Sequential Mechanism ◽  
Trna Methyltransferase ◽  
Gel Mobility Shift

ABSTRACT Down-regulation of expression of trmD, encoding the enzyme tRNA (guanosine-1)-methyltransferase, has shown that this gene is essential for growth of Streptococcus pneumoniae. The S. pneumoniae trmD gene has been isolated and expressed in Escherichia coli by using a His-tagged T7 expression vector. Recombinant protein has been purified, and its catalytic and physical properties have been characterized. The native enzyme displays a molecular mass of approximately 65,000 Da, suggesting that streptococcal TrmD is a dimer of two identical subunits. In fact, this characteristic can be extended to several other TrmD orthologs, including E. coli TrmD. Kinetic studies show that the streptococcal enzyme utilizes a sequential mechanism. Binding of tRNA by gel mobility shift assays gives a dissociation constant of 22 nM for one of its substrates, \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathit{tRNA}_{\mathit{CAG}}^{\mathit{Leu}}\) \end{document} . Other heterologous nonsubstrate tRNA species, like \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathit{tRNA}_{\mathit{GGT}}^{\mathit{Thr}}\) \end{document} , tRNAPhe, and \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathit{tRNA}_{\mathit{TGC}}^{\mathit{Ala}}\) \end{document} , bind the enzyme with similar affinities, suggesting that tRNA specificity is achieved via a postbinding event(s).

Molecular cloning, chromosome mapping and characterization of UBQLN3 a testis-specific gene that contains an ubiquitin-like domain

Gene ◽  
2000 ◽  
Vol 249 (1-2) ◽  
pp. 91-98 ◽  
Author(s):  
Darrell Conklin ◽  
Susan Holderman ◽  
Theodore E. Whitmore ◽  
Mark Maurer ◽  
Andrew L. Feldhaus
Keyword(s):  
Molecular Cloning ◽  
Chromosome Mapping ◽  
Specific Gene

scholarly journals Modulation of transcription and characterization of the promoter organization of the autotransporter adhesin heptosyltransferase and the autotransporter adhesin AIDA-I

Microbiology ◽  
2010 ◽  
Vol 156 (4) ◽  
pp. 1155-1166 ◽  
Author(s):  
Inga Benz ◽  
Tessa van Alen ◽  
Julia Bolte ◽  
Mirka E. Wörmann ◽  
M. Alexander Schmidt
Keyword(s):  
Protein Translocation ◽  
Growth Conditions ◽  
Limited Attention ◽  
Gram Negative Bacteria ◽  
Regulation Of Expression ◽  
Gene Encoding ◽  
E Coli ◽  
K 12 ◽  
Transcriptional Fusions

In Gram-negative bacteria, autotransporter proteins constitute the largest family of secreted proteins, and exhibit many different functions. In recent years, research has largely focused on mechanisms of autotransporter protein translocation, where several alternative models are still being discussed. In contrast, the biogenesis of only a few autotransporters has been studied and, likewise, regulation of expression has received only very limited attention. The glycosylated autotransporter adhesin involved in diffuse adherence (AIDA)-I system consists of the aah gene, encoding a specific autotransporter adhesin heptosyltransferase (AAH), and the aidA gene, encoding the autotransporter protein (AIDA-I). In this study, we investigated the promoter organization and transcription of these two genes using reporter plasmids carrying lacZ transcriptional fusions. The two genes, aah and aidA, are transcribed as a bicistronic message. However, aidA is additionally transcribed from its own promoter. There are two distinct start sites for each of the two genes. Interestingly, transcription of both genes is enhanced in hns and rfaH mutant backgrounds. Furthermore, we addressed the influence of environmental factors and different genetic backgrounds of Escherichia coli K-12 strains on transcription activity. We found that transcription varied considerably in different E. coli K-12 laboratory strains and under different growth conditions.

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