Molecular characterization of thirteen gyrA mutations conferring nalidixic acid resistance in Bacillus subtilis

1994 ◽  
Vol 244 (1) ◽  
pp. 97-103 ◽  
Author(s):  
N. Munakata ◽  
F. Morohoshi ◽  
M. Saitou ◽  
N. Yamazaki ◽  
K. Hayashi
Keyword(s):  
Bacillus Subtilis ◽  
Nalidixic Acid ◽  
Molecular Characterization ◽  
Acid Resistance ◽  
Nalidixic Acid Resistance

Phenotypic and genetic characterization of multidrug-resistant Salmonella Infantis strains isolated from broiler chicken meats in Turkey

Biologia ◽  
2011 ◽  
Vol 66 (3) ◽  
Author(s):  
Duygu Abbasoglu ◽  
Mustafa Akcelık
Keyword(s):  
Nalidixic Acid ◽  
Acid Resistance ◽  
Molecular Size ◽  
Multidrug Resistant ◽  
Chromosomal Dna ◽  
Gyra Gene ◽  
Class I Integrons ◽  
Phenotypic And Genetic Characterization ◽  
Nalidixic Acid Resistance

AbstractTwenty Salmonella Infantis strains resistant against kanamycin, tetracycline, neomycin, spectinomycin, sulphonamide, nalidixic acid and trimethoprim were selected for this study out of 103 Salmonella strains isolated from broiler samples collected from several markets in the Bolu and Ankara regions of Turkey. The resistance genes aadA1, aphA1, sul1, tet(A), dfrA5/dfrA14 and gyrA were determined for these multidrug-resistant S. Infantis strains. S. Infantis strains contained a mega plasmid with the molecular size of 206 kb. The strains were divided into three groups according to the pulsed field gel electrophoresis patterns of XbaI digested chromosomal DNA. A Ser83→Tyr83 point mutation was found in the gyrA gene of all quinolone-resistant isolates. Filter mating experiments showed that 206 kb plasmid transferred nalidixic acid resistance associated with class I integrons.

scholarly journals Susceptibility to patterns of ciprofloxacin among nalidixic acid-resistant Salmonella isolates collected in Banepa, Nepal from enteric fever patients

2016 ◽  
Vol 89 (2) ◽  
Author(s):  
Girija Roka ◽  
Subash Pandaya ◽  
Md. Reyad-ul Ferdous ◽  
Manson Pandey ◽  
Naba Raj Pokhrel ◽  
...  
Keyword(s):  
Nalidixic Acid ◽  
Salmonella Enterica ◽  
Enteric Fever ◽  
Inhibitory Concentration ◽  
Acid Resistance ◽  
Dilution Method ◽  
Disc Diffusion ◽  
Antimicrobial Sensitivity ◽  
Broth Dilution ◽  
Nalidixic Acid Resistance

The present study determined the susceptibility to ciprofloxacin of nalidixic acid resistant <em>Salmonella</em> (NARS) isolated from enteric fever patients at Scheer Memorial Hospital, Banepa, Nepal, from June 2012 to December 2012. The antimicrobial sensitivity to nalidixic acid and ciprofloxacin was determined using modified Kirby-Bauer disc diffusion and broth dilution method according to the guidelines of the Clinical and Laboratory Standard Institute. <em>Salmonella</em> was isolated from 34 out of 992 (3.43%) blood cultures collected during the study period, and 10 (29.4%) isolates were identified as <em>Salmonella enterica</em> serotype Typhi, while 24 (70.6%) were identified as <em>Salmonella enterica</em> serotype Paratyphi. Out of the total isolates, 31 (91.2%) were nalidixic acid-resistant <em>Salmonella</em> (NARS). Among NARS, the minimum inhibitory concentration values for ciprofloxacin ranged from 0.25 to 2 mg/L and were constantly higher than those shown by the nalidixic acid-susceptible <em>Salmonella</em>. Therefore, in typhoid <em>Salmonella</em> nalidixic acid resistance may be the indicator of decreased susceptibility to ciprofloxacin.

Isolation and Molecular Characterization of Thermostable Phytase from Bacillus subtilis (BSPhyARRMK33)

2015 ◽  
Vol 175 (6) ◽  
pp. 3058-3067 ◽  
Author(s):  
Chinreddy Subramanyam Reddy ◽  
V. Mohan Murali Achary ◽  
Mrinalini Manna ◽  
Jitender Singh ◽  
Tanushri Kaul ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Molecular Characterization

scholarly journals Differential Effects of Temperature on Natural Transformation to Erythromycin and Nalidixic Acid Resistance in Campylobacter coli

2008 ◽  
Vol 74 (19) ◽  
pp. 6121-6125 ◽  
Author(s):  
Joo-Sung Kim ◽  
Jae-Won Kim ◽  
S. Kathariou
Keyword(s):  
Nalidixic Acid ◽  
Acid Resistance ◽  
Campylobacter Coli ◽  
Limited Information ◽  
Erythromycin Resistance ◽  
Highly Efficient ◽  
Animal Hosts ◽  
Effects Of Temperature ◽  
The Impact ◽  
Nalidixic Acid Resistance

ABSTRACT Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic acid resistance. Frequency of transformation was not significantly different between microaerobic (5 to 10% CO2) and aerobic conditions. However, C. coli was transformed to erythromycin resistance at a significantly higher frequency at 42�C than at 25�C (P < 0.05), and few or no transformants were obtained at 25�C. In contrast, transformation to nalidixic acid resistance was highly efficient at both 42�C and 25�C and was similar or, at the most, fourfold higher at 42�C than at 25�C. DNase I treatment experiments suggested that steps both prior and subsequent to internalization of DNA were influenced by temperature in the case of transformation of C. coli to erythromycin resistance. However, the moderately increased (fourfold) frequency of transformation to nalidixic acid resistance at 42�C compared to that at 25�C was exclusively associated with steps prior to DNA internalization. These findings suggest that transformation to erythromycin resistance may be significantly more frequent in the gastrointestinal tract of hosts such as poultry (at 42�C) than in other habitats characterized by lower temperatures, whereas transformation to nalidixic acid resistance may be highly efficient both within and outside the animal hosts.

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