Variation in tritiated thymidine uptake during DNA synthesis in the adrenal cortex

Histochemie ◽  
1971 ◽  
Vol 28 (2) ◽  
pp. 99-102 ◽  
Author(s):  
N. A. Wright
Keyword(s):  
Dna Synthesis ◽  
Adrenal Cortex ◽  
Tritiated Thymidine ◽  
Thymidine Uptake ◽  
Tritiated Thymidine Uptake

Damage by Dichlorvos of Human Lymphocyte DNA

1980 ◽  
Vol 66 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Paolo Perocco ◽  
Angela Fini
Keyword(s):  
Dna Synthesis ◽  
Human Lymphocyte ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Ultraviolet Rays ◽  
Thymidine Uptake ◽  
Short Term ◽  
In Vitro System ◽  
Tritiated Thymidine Uptake

The action of dichlorvos (2.2-dichlorovinyldimethyl phosphate) was studied with a short-term in vitro system which utilizes human lymphocytes. The parameters studied were the action exerted by the pesticide on scheduled (semiconservative) and unscheduled (reparative) DNA synthesis measured as tritiated thymidine uptake. The results obtained show that dichlorvos affects semiconservative DNA synthesis, damages human lymphocyte DNA inducing low reparative synthesis, and interferes with DNA repair processes after damage exerted by ultraviolet rays.

Permeability alterations in amphibian embryos caused by salt solution and measured by tritiated thymidine uptake

Development ◽  
1964 ◽  
Vol 12 (3) ◽  
pp. 407-424
Author(s):  
Clarence A. Loeffler ◽  
Malcolm C. Johnston
Keyword(s):  
Salt Solution ◽  
Tap Water ◽  
Tritiated Thymidine ◽  
Permeability Barrier ◽  
Thymidine Uptake ◽  
Surface Coat ◽  
Amphibian Embryo ◽  
Amphibian Embryos ◽  
Tritiated Thymidine Uptake ◽  
Permeability Alterations

In attempts to label the nuclei of intact amphibian embryos with tritiated thymidine we found, in agreement with other authors (Tencer, 1961; Quertier, 1962; Chibon, 1962), that when the nucleoside is dissolved in tap water or media of low salt concentration it fails to enter the embryo. However, when tritiated thymidine was either injected into the embryo or applied to isolated tissues immersed in salt solution, the marker diffused readily through the tissues. Evidently the outer surface of the amphibian embryo is furnished with a special permeability barrier which prevents or reduces the entrance of these molecules. This barrier corresponds most likely to what Holtfreter (1943a, 1943b) has described as the ‘surface coat’. In order that the results of our present experiments may be better understood some of the pertinent properties of the coat will be reviewed. A protective coat, while still absent in the ovarian egg, arises after the egg is deposited in water.

Mediators of the mitogenic action of human V1vascular vasopressin receptors

2000 ◽  
Vol 279 (5) ◽  
pp. H2529-H2539 ◽  
Author(s):  
Marc Thibonnier ◽  
Doreen M. Conarty ◽  
Christine L. Plesnicher
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Protein Kinase ◽  
Dna Synthesis ◽  
Cell Cycle Progression ◽  
Tritiated Thymidine ◽  
Chinese Hamster ◽  
Mitogen Activated Protein Kinase ◽  
Thymidine Uptake ◽  
Mitogenic Action

Arginine vasopressin (AVP) activation of V1 vascular receptors (V1Rs) stimulates cell growth and proliferation in different tissues via cellular signaling pathways that remain to be identified. To explore the intracellular mediators of the mitogenic action of V1R, Chinese hamster ovary (CHO) cells were stably transfected with the human V1R cDNA clone we isolated previously. We assessed AVP effects on kinase activation (immunoblotting with phosphospecific antibodies), DNA synthesis (tritiated thymidine uptake), cell cycle progression (flow cytometry analysis after nuclear labeling with propidium iodide), and cell proliferation (conversion of the colorimetric reagent MTS) in the presence or absence of various pathway inhibitors. AVP stimulation of V1Rs leads to the phosphorylation of several kinases, an increase in DNA synthesis, a progression through the S and G2–M phases of the cell cycle, and an increase in cell proliferation. The mediators of the mitogenic action of V1R activation included calcium mobilization, coupling to a Gq protein, and the simultaneous and parallel activation of several kinases, mainly calcium/calmodulin-dependent kinase II, phosphatidylinositol 3 kinase, protein kinase C, and p42/p44 mitogen-activated protein kinase.

Vandellia Cordifolia Regulated Cell Proliferation and Cytokines Production in Human Mononuclear Cells

2000 ◽  
Vol 28 (03n04) ◽  
pp. 313-323 ◽  
Author(s):  
An-Pang Lin ◽  
Wei-Jern Tsai ◽  
Chi-Yen Fan ◽  
Ming-Jen Lee ◽  
Yuh-Chi Kuo
Keyword(s):  
Cell Cycle Progression ◽  
Mononuclear Cells ◽  
Tritiated Thymidine ◽  
Interleukin 2 ◽  
Interferon Γ ◽  
Thymidine Uptake ◽  
Dependent Manner ◽  
Human Mononuclear Cells ◽  
Tritiated Thymidine Uptake ◽  
Ifn Γ

Vandellia cordifolia (V. cordifolia) used for treatment inflammation in traditional Chinese medicine was selected for immunopharmacological activity test. The effects of V. cordifolia extracted fractions on human mononuclear cells (HMNC) proliferation were determined by tritiated thymidine uptake. The results indicated that VC-ME fraction suppressed HMNC proliferation activated with phytohemagglutinin (PHA) and stimulated cell cycle progression was arrested at the G0/G1 stage. The inhibitory mechanisms may involve the blocking of interleukin-2 (IL-2) and interferon-γ (IFN-γ) production, since VC-ME suppressed IL-2 and IFN-γ production of HMNC in a dose-dependent manner. Therefore, it is suggested that immunomodulatory agents are contained in V. cordifolia.

scholarly journals OBSERVATIONS ON THE UPTAKE OF TRITIATED THYMIDINE IN THE PRONUCLEI OF FERTILIZED SAND DOLLAR EMBRYOS

1961 ◽  
Vol 10 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Eva B. Simmel ◽  
David A. Karnofsky
Keyword(s):  
Dna Synthesis ◽  
Nuclear Localization ◽  
Tritiated Thymidine ◽  
Nuclear Material ◽  
Thymidine Uptake ◽  
Sand Dollar ◽  
Male And Female ◽  
Daughter Cells ◽  
Minute Period ◽  
Independent Replication

Following fertilization of the egg of the sand dollar Echinarachnius parma, tritiated thymidine (H3TDR) was taken up independently by the male and female pronuclei beginning within about 15 to 20 minutes, and the labeled pronuclei fused at about 30 to 40 minutes. At cleavage 90 minutes later the labeled nuclear material was distributed to both daughter cells. Unfertilized eggs and sperm exposed to H3TDR did not show nuclear localization of thymidine. DNA replication, thus, is initiated in the haploid pronuclei shortly after fertilization and prior to fusion. The major portion of DNA synthesis, as evidenced by thymidine uptake, appears to be during a 20 to 30 minute period after fertilization. Fertilization is associated with the activation of a mechanism which initiates early and independent replication of DNA in both the male and female pronuclei.

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