Sequence of the cellular T-DNA in the untransformed genome of Nicotiana glauca that is homologous to ORFs 13 and 14 of the Ri plasmid and analysis of its expression in genetic tumors of N. glauca x N. langsdorffii

1994 ◽  
Vol 243 (6) ◽  
pp. 706-710 ◽  
Author(s):  
Seishirō Aoki ◽  
Akiyoshi Kawaoka ◽  
Masami Sekine ◽  
Takanari Ichikawa ◽  
Tomomichi Fujita ◽  
...  
Keyword(s):  
Nicotiana Glauca ◽  
Ri Plasmid ◽  
Genetic Tumors

Evidence for the expression of the rol genes of Nicotiana glauca in genetic tumors of N. glauca x N. langsdorflli

1990 ◽  
Vol 220 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Takanari Ichikawa ◽  
Yoshihiro Ozeki ◽  
Kunihiko Syöno
Keyword(s):  
Rol Genes ◽  
Nicotiana Glauca ◽  
Genetic Tumors

scholarly journals Tobacco mild green mosaic virus found naturally infecting Nicotiana glauca in Brazil

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Gabriel Madoglio Favara ◽  
Viviana Marcela Camelo-García ◽  
Tatsuya Nagata ◽  
João Marcos Fagundes Silva ◽  
Mariana Saito ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Glauca

scholarly journals The Presence and Characterization of a virF Gene on Agrobacterium vitis Ti Plasmids

1998 ◽  
Vol 11 (5) ◽  
pp. 429-433 ◽  
Author(s):  
B. Schrammeijer ◽  
J. Hemelaar ◽  
P. J. J. Hooykaas
Keyword(s):  
Amino Acids ◽  
Agrobacterium Tumefaciens ◽  
Molecular Mass ◽  
Promoter Region ◽  
Consensus Sequence ◽  
Tumor Formation ◽  
Nicotiana Glauca ◽  
Agrobacterium Vitis ◽  
Ti Plasmids

Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plas-mids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca.

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