The regulatory gene nit-2 of Neurospora crassa complements a nnu mutant of Gibberella zeae (Fusarium graminearum)

1992 ◽  
Vol 235 (2-3) ◽  
pp. 458-462 ◽  
Author(s):  
Martin B. Dickman ◽  
John F. Leslie
Keyword(s):  
Neurospora Crassa ◽  
Fusarium Graminearum ◽  
Regulatory Gene ◽  
Gibberella Zeae

scholarly journals Técnicas de aplicação de fungicida em trigo para o controle de Giberela (Gibberella zeae)

2003 ◽  
Vol 33 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Edivan Panisson ◽  
Walter Boller ◽  
Erlei Melo Reis ◽  
Laércio Hoffmann
Keyword(s):  
Fusarium Graminearum ◽  
Gibberella Zeae

Durante muitos anos, a giberela, causada pelo fungo Gibberella zeae (anamorfo Fusarium graminearum), foi considerada de importância secundária no sul do Brasil. O aumento da intensidade e da freqüência de ocorrência, tornou a giberela uma das doenças de maior importância na cultura do trigo. A giberela é uma doença de infecção floral e mesmo os fungicidas sistêmicos recomendados apresentam apenas efeito protetor das anteras. Os objetivos do presente trabalho foram avaliar a eficiência de controle e os efeitos nos grãos colhidos, de dois tipos de pontas de pulverização (leque e duplo leque), diferentes arranjos dos bicos na barra de aplicação e dois volumes de calda. A desuniformidade da antese aparece como um dos principais fatores envolvidos com a baixa eficiência dos fungicidas. Nos experimentos, realizados no ano 2000, a aplicação dos tratamentos reduziu significativamente a incidência, o número de espiguetas gibereladas e a severidade da doença, aumentando o rendimento de grãos. As pontas de pulverização, seus arranjos na barra e os volumes de calda utilizados comportaram-se de maneira semelhante em todas as variáveis avaliadas. O incremento no rendimento de grãos, obtido em relação à testemunha, sugere que se deve recomendar a aplicação de fungicidas para o controle da giberela, utilizando pontas que geram gotas finas a médias com volume de calda de 200 L.ha-1.

Physiological and Environmental Aspects of Ascospore Discharge in Gibberella zeae (Anamorph Fusarium graminearum)

Mycologia ◽  
2002 ◽  
Vol 94 (2) ◽  
pp. 181 ◽  
Author(s):  
Frances Trail ◽  
Haixin Xu ◽  
Rachel Loranger ◽  
David Gadoury
Keyword(s):  
Fusarium Graminearum ◽  
Gibberella Zeae ◽  
Environmental Aspects ◽  
Ascospore Discharge

scholarly journals The Spread of a Released Clone of Gibberella zeae from Different Amounts of Infested Corn Residue

Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1458-1464 ◽  
Author(s):  
Melissa D. Keller ◽  
Wade E. Thomason ◽  
David G. Schmale
Keyword(s):  
Fusarium Graminearum ◽  
Wheat Cultivar ◽  
Gibberella Zeae ◽  
Corn Stalk ◽  
Head Blight ◽  
Inoculum Sources ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Corn Residue ◽  
Moderately Resistant

Corn residue is a significant source of inoculum for epidemics of Fusarium head blight (FHB) in wheat and barley, but little is known about the influence of different amounts of corn residue on FHB. We monitored the spread of a released clone of Gibberella zeae (Fusarium graminearum), causal agent of FHB, from small 0.84-m-diameter research plots containing 45, 200, or 410 g of infested corn stalk pieces in winter wheat and barley fields in Virginia over 3 years (2008 to 2010). The fungus was recaptured through the collection of wheat and barley spikes at 0 and 3 m from the source and the released clone was identified in heterogeneous background populations using amplified fragment length polymorphisms. Results showed a slightly greater intensity of recovery of the clone at a greater distance when more infested residue was present. Plots containing larger amounts of inoculum (410 g) generally resulted in a smaller decline of recovery of the clone at 3 m from the source, indicating a greater spread from the larger inoculum source. The clone was also recovered at distances ≥18 m from inoculum sources. Larger amounts of corn residue generally had less influence on clone recovery in plots containing a moderately resistant wheat cultivar than those containing a susceptible wheat cultivar.

scholarly journals Isolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system.

1989 ◽  
Vol 9 (9) ◽  
pp. 4113-4117 ◽  
Author(s):  
G J Sorger ◽  
D Brown ◽  
M Farzannejad ◽  
A Guerra ◽  
M Jonathan ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Gene Product ◽  
Regulatory Gene ◽  
Nitrate Assimilation ◽  
Gene Products ◽  
Specific Pathway

Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.

scholarly journals Mutational analysis of the DNA-binding domain of the CYS3 regulatory protein of Neurospora crassa.

1991 ◽  
Vol 11 (9) ◽  
pp. 4356-4362 ◽  
Author(s):  
M N Kanaan ◽  
G A Marzluf
Keyword(s):  
Dna Binding ◽  
Neurospora Crassa ◽  
Mutational Analysis ◽  
Regulatory Gene ◽  
Regulatory Protein ◽  
Binding Activity ◽  
Binding Domain ◽  
Site Directed Mutagenesis ◽  
Dna Binding Domain ◽  
Dna Binding Activity

cys-3, the major sulfur regulatory gene of Neurospora crassa, activates the expression of a set of unlinked structural genes which encode sulfur catabolic-related enzymes during conditions of sulfur limitation. The cys-3 gene encodes a regulatory protein of 236 amino acid residues with a leucine zipper and an upstream basic region (the b-zip region) which together may constitute a DNA-binding domain. The b-zip region was expressed in Escherichia coli to examine its DNA-binding activity. The b-zip domain protein binds to the promoter region of the cys-3 gene itself and of cys-14, the sulfate permease II structural gene. A series of CYS3 mutant proteins obtained by site-directed mutagenesis were expressed and tested for function, dimer formation, and DNA-binding activity. The results demonstrate that the b-zip region of cys-3 is critical for both its function in vivo and specific DNA-binding in vitro.

Gene expression shifts during perithecium development in Gibberella zeae (anamorph Fusarium graminearum), with particular emphasis on ion transport proteins

2007 ◽  
Vol 44 (11) ◽  
pp. 1146-1156 ◽  
Author(s):  
Heather E. Hallen ◽  
Marianne Huebner ◽  
Shin-Han Shiu ◽  
Ulrich Güldener ◽  
Frances Trail
Keyword(s):  
Gene Expression ◽  
Ion Transport ◽  
Fusarium Graminearum ◽  
Transport Proteins ◽  
Gibberella Zeae
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