Comparison of RADM dry deposition algorithms with a site-specific method for inferring dry deposition

1989 ◽  
Vol 44 (3-4) ◽  
pp. 273-293 ◽  
Author(s):  
M. L. Wesely ◽  
B. M. Lesht
Keyword(s):  
Dry Deposition ◽  
Specific Method ◽  
Site Specific ◽  
A Site

Site-specific control of silica mineralization on DNA using a designed peptide

2016 ◽  
Vol 52 (21) ◽  
pp. 4010-4013 ◽  
Author(s):  
Makoto Ozaki ◽  
Kazuma Nagai ◽  
Hiroto Nishiyama ◽  
Takaaki Tsuruoka ◽  
Satoshi Fujii ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Organic Compounds ◽  
Peptide Nucleic Acids ◽  
Inorganic Compounds ◽  
Specific Method ◽  
Site Specific ◽  
A Site ◽  
Specific Control

We developed a site-specific method for precipitating inorganic compounds using organic compounds, DNA, and designed peptides with peptide nucleic acids (PNAs).

ADVANCE PLANNING FOR DISPERSANT USE

1987 ◽  
Vol 1987 (1) ◽  
pp. 329-333 ◽  
Author(s):  
June Lindstedt-Siva
Keyword(s):  
State Government ◽  
Task Force ◽  
American Petroleum Institute ◽  
Specific Method ◽  
Marine Environments ◽  
Coastal Habitat ◽  
Site Specific ◽  
Advance Planning ◽  
American Society ◽  
A Site

ABSTRACT Dispersant use is most effective in the early stages of an oil spill, yet because there is thought to be so much case-by-case variation, decisions about whether and where to use dispersants have been made only after a spill occurs. These decisions require, at a minimum, hours, and may require days. Two major efforts to shorten this decisionmaking process have recently been completed. A multidisciplinary, multiagency task force under the auspices of the American Society for Testing and Materials developed ecologically based guidelines for dispersant use in marine environments. The guidelines for 13 different marine and coastal habitat types consider dispersant use both to protect and to clean the habitat. They also identify those habitats that should be most protected in the event of a spill. These guidelines should be useful references for regional response teams and other spill response planners as they develop site-specific dispersant use plans. The second project was developed by an American Petroleum Institute task force and contractor with input from federal and state government agencies. It is a site-specific method for planning whether to use dispersants in marine environments. It divides an offshore region into “dispersant use zones” based on ecological considerations. The method was applied to selected areas offshore southern California as a test.

scholarly journals Expanding N-Glycopeptide Identifications by Fragmentation Prediction and Glycome Network Smoothing

2021 ◽  
Author(s):  
Joshua Klein ◽  
Luis Carvalho ◽  
Joseph Zaia
Keyword(s):  
Search Space ◽  
Specific Method ◽  
Challenging Problem ◽  
Site Specific ◽  
Recent Innovation ◽  
Uncertainty Estimates ◽  
Layered Approach ◽  
A Site ◽  
Performance Gains ◽  
Made In

AbstractAccurate glycopeptide identification in mass spectrometry-based glycoproteomics is a challenging problem at scale. Recent innovation has been made in increasing the scope and accuracy of glycopeptide identifications, with more precise uncertainty estimates for each part of the structure. We present a layered approach to glycopeptide fragmentation modeling that improves N-glycopeptide identification in samples without compromising identification quality, and a site-specific method to increase the depth of the glycoproteome confidently identifiable even further. We demonstrate our techniques on a pair of previously published datasets, showing the performance gains at each stage of optimization, as well as its flexibility in glycome definition and search space complexity. These techniques are provided in the open-source glycomics and glycoproteomics platform GlycReSoft available at https://github.com/mobiusklein/glycresoft.

scholarly journals Functional expression of a yeast mitochondrial intron-encoded protein requires RNA processing at a conserved dodecamer sequence at the 3' end of the gene.

1989 ◽  
Vol 9 (4) ◽  
pp. 1507-1512 ◽  
Author(s):  
H Zhu ◽  
H Conrad-Webb ◽  
X S Liao ◽  
P S Perlman ◽  
R A Butow
Keyword(s):  
Genetic Analysis ◽  
Rna Processing ◽  
Fold Increase ◽  
Functional Expression ◽  
Rrna Gene ◽  
Yeast Mitochondria ◽  
Wild Type ◽  
Site Specific ◽  
Var1 Gene ◽  
A Site

All mRNAs of yeast mitochondria are processed at their 3' ends within a conserved dodecamer sequence, 5'-AAUAAUAUUCUU-3'. A dominant nuclear suppressor, SUV3-I, was previously isolated because it suppresses a dodecamer deletion at the 3' end of the var1 gene. We have tested the effects of SUV3-1 on a mutant containing two adjacent transversions within a dodecamer at the 3' end of fit1, a gene located within the 1,143-base-pair intron of the 21S rRNA gene, whose product is a site-specific endonuclease required in crosses for the quantitative transmission of that intron to 21S alleles that lack it. The fit1 dodecamer mutations blocked both intron transmission and dodecamer cleavage, neither of which was suppressed by SUV3-1 when present in heterozygous or homozygous configurations. Unexpectedly, we found that SUV3-1 completely blocked cleavage of the wild-type fit1 dodecamer and, in SUV3-1 homozygous crosses, intron conversion. In addition, SUV3-1 resulted in at least a 40-fold increase in the amount of excised intron accumulated. Genetic analysis showed that these phenotypes resulted from the same mutation. We conclude that cleavage of a wild-type dodecamer sequence at the 3' end of the fit1 gene is essential for fit1 expression.

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