Role of Na+ in growth, respiration and membrane transport in the marine bacterium Pseudomonas doudoroffii 70

1989 ◽  
Vol 153 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Gesine A. Wisse ◽  
Robert A. MacLeod
1991 ◽  
Vol 37 (6) ◽  
pp. 433-439 ◽  
Author(s):  
Marc Berthelet ◽  
Robert A. MacLeod

Deleya aesta required Na+ for the uptake of 8 of 11 metabolites tested; the other three were transported at low rates in the absence of Na+ but at much higher rates in its presence. The optimal concentration of Na+ for maximum rate of transport of all the metabolites was 200 to 300 mM. Higher concentrations added as NaCl inhibited transport to the same extent as equiosomolar concentrations of other salts and sucrose. Li+ but not K+ could replace Na+ for the uptake of some metabolites but was only one-half as effective. Competition studies indicated that a number of different transport systems were involved in uptake. Inhibitor studies of succinate, L-alanine, and D-glucose accumulation (the last in both the presence and absence of Na+) suggested that for each a membrane potential was required. The relationship between rates of transport and oxidation of succinate by intact cells at various Na+ concentrations indicated that the Na+ requirement for oxidation reflected the Na+ requirement for transport. For D-glucose, the relationship was more complex, sinced over a narrow, low concentration range, Na+ inhibited respiration but not transport. Evidence for the presence in D. aesta 134 of a Na+-activated NADH–quinone acceptor oxidoreductase was obtained. Key words: Deleya aesta, marine bacteria, sodium, membrane transport, respiration.


2001 ◽  
Vol 13 (4) ◽  
pp. 485-492 ◽  
Author(s):  
Anne Simonsen ◽  
Andrew E Wurmser ◽  
Scott D Emr ◽  
Harald Stenmark
Keyword(s):  

2004 ◽  
Vol 91 (12) ◽  
pp. 2079-2085 ◽  
Author(s):  
A R Buursma ◽  
I J van Dillen ◽  
A van Waarde ◽  
W Vaalburg ◽  
G A P Hospers ◽  
...  

2010 ◽  
Vol 99 (3) ◽  
pp. 1336-1345 ◽  
Author(s):  
Koji Yano ◽  
Yoshie Masaoka ◽  
Makoto Kataoka ◽  
Shinji Sakuma ◽  
Shinji Yamashita

2005 ◽  
Vol 319 (1-3) ◽  
pp. 342-349 ◽  
Author(s):  
Alexander M. Berezhkovskii ◽  
Sergey M. Bezrukov

Metabolism ◽  
1995 ◽  
Vol 44 (6) ◽  
pp. 719-724 ◽  
Author(s):  
Gianni Biolo ◽  
Xiao-Jun Zhang ◽  
Robert R. Wolfe

Sign in / Sign up

Export Citation Format

Share Document