Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L. cv. ‘Bintje’)

1983 ◽  
Vol 65 (4) ◽  
pp. 329-338 ◽  
Author(s):  
K. Sree Ramulu ◽  
P. Dijkhuis ◽  
S. Roest
PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199716 ◽  
Author(s):  
Juan David Santa ◽  
Jhon Berdugo-Cely ◽  
Liliana Cely-Pardo ◽  
Mauricio Soto-Suárez ◽  
Teresa Mosquera ◽  
...  

Author(s):  
Hemant B. Kardile ◽  
Virupaksh U. Patil ◽  
Nirmal K. Sharma ◽  
G. Vanishree ◽  
Deodhar Singh ◽  
...  

2022 ◽  
Vol 295 ◽  
pp. 110859
Author(s):  
Salej Sood ◽  
Vinay Bhardwaj ◽  
Kumar N. Chourasia ◽  
Ratna Preeti Kaur ◽  
Vinod Kumar ◽  
...  

Author(s):  
Emmy Chepkoech ◽  
Miriam G. Kinyua ◽  
Oliver Kiplagat ◽  
Julius Ochuodho ◽  
Souleymane Bado ◽  
...  

Aims: Potato (Solanum tuberosum L.) is the most important staple food in the world and plays an important role in food and nutritional security. Induced mutation generates variation within potato germplasm to widen the genetic base for breeding purposes. Polyploidy modifies both the genotype and phenotype of an organism, generating diverse changes that consequently transform the potato production. Potato has chromosomes with different ploidy levels which can be determined by counting chloroplasts in stomatal guard cells. Study Design:  The study was carried out in completely randomized block design. Place and Duration of Study: Department of Biotechnology, University of Eldoret, between February 2015 and July 2016. Methodology: The study involved 163 potato mutants developed from three commercially grown Kenyan potato varieties; Asante, Mpya, and Sherekea irradiated using gamma rays from 60Co source under different dose rates. Three middle leaves of greenhouse-grown plants were randomly selected for chloroplast counts in ten pairs of stomata guard cells on the lower surface of the leaf. Data on the number of chloroplast counts per mutant was calculated as a percentage of the parents or control and descriptive analysis. Results: The results indicate that the number of ploidy level distribution was decreasing in diploids and triploids and were increasing in tetraploids from M1V1, M1V2 to M1V3 in all the potato mutant populations. Conclusion: This shows that mutation induction generates genetic variations from which desired mutants may be selected based on the appropriate breeding strategies.


Euphytica ◽  
1978 ◽  
Vol 27 (2) ◽  
pp. 385-388 ◽  
Author(s):  
J. G. Th. Hermsen ◽  
J. Sonneveld ◽  
D. Vreugdenhil ◽  
D. van Ballegooijen ◽  
E. Staas-Ebregt

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