Significant improvement of androgenetic haploid and doubled haploid induction from wheat plants treated with a chemical hybridization agent

1987 ◽  
Vol 74 (3) ◽  
pp. 289-297 ◽  
Author(s):  
E. Picard ◽  
C. Hours ◽  
S. Gr�goire ◽  
T. H. Phan ◽  
J. P. Meunier
Keyword(s):  
Doubled Haploid ◽  
Haploid Induction ◽  
Chemical Hybridization Agent ◽  
Androgenetic Haploid

Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
R. K Khulbe ◽  
A. Pattanayak ◽  
Lakshmi Kant ◽  
G. S. Bisht ◽  
M. C. Pant ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Sweet Corn ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Line Production ◽  
Haploid Inducer ◽  
Breeding Programmes ◽  
Source Populations ◽  
Developmental Phases

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

scholarly journals Doubled haploid technology for line development in maize: technical advances and prospects

2019 ◽  
Vol 132 (12) ◽  
pp. 3227-3243 ◽  
Author(s):  
Vijay Chaikam ◽  
Willem Molenaar ◽  
Albrecht E. Melchinger ◽  
Prasanna M. Boddupalli
Keyword(s):  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Production Costs ◽  
Great Promise ◽  
Success Rates ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Breeding Programs ◽  
Line Production

Key Message Increased efficiencies achieved in different steps of DH line production offer greater benefits to maize breeding programs. Abstract Doubled haploid (DH) technology has become an integral part of many commercial maize breeding programs as DH lines offer several economic, logistic and genetic benefits over conventional inbred lines. Further, new advances in DH technology continue to improve the efficiency of DH line development and fuel its increased adoption in breeding programs worldwide. The established method for maize DH production covered in this review involves in vivo induction of maternal haploids by a male haploid inducer genotype, identification of haploids from diploids at the seed or seedling stage, chromosome doubling of haploid (D0) seedlings and finally, selfing of fertile D0 plants. Development of haploid inducers with high haploid induction rates and adaptation to different target environments have facilitated increased adoption of DH technology in the tropics. New marker systems for haploid identification, such as the red root marker and high oil marker, are being increasingly integrated into new haploid inducers and have the potential to make DH technology accessible in germplasm such as some Flint, landrace, or tropical material, where the standard R1-nj marker is inhibited. Automation holds great promise to further reduce the cost and time in haploid identification. Increasing success rates in chromosome doubling protocols and/or reducing environmental and human toxicity of chromosome doubling protocols, including research on genetic improvement in spontaneous chromosome doubling, have the potential to greatly reduce the production costs per DH line.

Effects of consecutive androgeneses on morphology and fertility in Nicotiana sylvestris

1983 ◽  
Vol 61 (7) ◽  
pp. 2038-2046 ◽  
Author(s):  
R. De Paepe ◽  
D. Prat ◽  
J. Knight
Keyword(s):  
Doubled Haploid ◽  
Pollen Fertility ◽  
Doubled Haploids ◽  
Pollen Grains ◽  
Nicotiana Sylvestris ◽  
Somatic Tissue ◽  
Source Line ◽  
Doubled Haploid Plants ◽  
Androgenetic Haploid ◽  
Haploid Plants

In Nicotiana sylvestris androgenetic haploid and doubled-haploid plants regenerated from isolated pollen grains show characteristic modifications in leaves and flowers as compared with the pollen source line. During successive cycles of androgenesis, their average dimensions decrease regularly up to the fifth cycle. Afterwards no significant decrease is observed, but other kinds of abnormalities appear, such as foliar outgrowths developing from leaf veins, in haploid and doubled-haploid plants, or from the somatic tissue of the ovules in haploids only. Doubled-haploid plants transmit their abnormalities through the first and second generations of selfing. Their fertility is greatly reduced because of physiological deficiencies when the plants are used as females, and low pollen fertility. Pollen from doubled haploids also has inferior androgenic capabilities.

scholarly journals A genotype independent DMP-HI system in dicot crops

2021 ◽  
Author(s):  
Yu Zhong ◽  
Baojian Chen ◽  
Dong Wang ◽  
Xijian Zhu ◽  
Yuwen Wang ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Haploid Induction ◽  
Crop Breeding ◽  
Single Generation ◽  
The Way

Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, which significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is been widely and efficiently used in maize and was recently extended to several monocot crops. However, a similar generic and efficient HI system is still lacking in dicot crops. Here we show that genotype-independent in vivo HI can be triggered by mutation of DMP genes in tomato, rapeseed and tobacco with HI rates of ~1.9%, 2.4% and 1.2%, respectively. The DMP-HI system offers a robust DH technology to facilitate variety improvement in these crops. The success of this approach and the conservation of DMP genes paves the way for a generic and efficient genotype-independent HI system in other dicot crops.

scholarly journals Improvements of doubled haploid production protocol for white cabbage (Brassica oleracea var. capitata L.)

2018 ◽  
Vol 30 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Katarina Rudolf Pilih ◽  
Urška Karolina Potokar ◽  
Borut Bohanec
Keyword(s):  
Brassica Oleracea ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Petri Dish ◽  
Abnormal Development ◽  
Haploid Induction ◽  
Embryo Formation ◽  
White Cabbage ◽  
Dh Lines ◽  
Further Development

Abstract Protocols leading to the development of doubled haploid (DH) lines by microspore culture are widely used in white cabbage (Brassica oleracea var. capitata L.), but efficiency varies according to the cultivar and induction procedure. Forty different genotypes consisting of F1 cultivars and their crosses with responsive doubled haploid lines were tested to evaluate the androgenic response. In total, 20,032 embryos were produced. On average, the haploid induction response of F1 cultivars was 7.0 embryos/Petri dish, but the average of these hybrids crossed to responsive DH lines was 26.6 embryos/Petri dish. In seven reciprocal crosses, a difference was observed in just one, meaning that the maternal effect probably has a minor influence on haploid embryogenesis in cabbage. Addition of 0.02% activated charcoal (AC) to the induction media increased embryo formation in several low-responsive genotypes, but its effect on embryo formation of high-responsive genotypes was predominantly negative, although larger embryos were formed on media containing AC than without AC. Further development into plantlets was tested by two procedures. Formed embryos were either transferred directly to regeneration medium or treated with abscisic acid and desiccated for 4 weeks. Regrowth and further development reached on average 15.5 and 57.6%, for the first and second procedures, respectively. Plantlets developed by direct transfer often exhibited abnormal development or hyperhydricity, unlike the desiccated embryos. Spontaneous diploidisation of embryos reached 42.5% in total and was not affected by AC added to the induction media.

scholarly journals Development of In Vivo Haploid Inducer Lines for Screening Haploid Immature Embryos in Maize

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 739
Author(s):  
Chen Chen ◽  
Zijian Xiao ◽  
Junwen Zhang ◽  
Wei Li ◽  
Jinlong Li ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Immature Embryos ◽  
Haploid Induction ◽  
Haploid Induction Rate ◽  
Induction Rate ◽  
Haploid Inducer ◽  
Breeding Populations ◽  
Selection For ◽  
Doubled Haploid Breeding

Doubled haploid technology is widely applied in maize. The haploid inducer lines play critical roles in doubled haploid breeding. We report the development of specialized haploid inducer lines that enhance the purple pigmentation of crossing immature embryos. During the development of haploid inducer lines, two breeding populations derived from the CAU3/S23 and CAU5/S23 were used. Molecular marker-assisted selection for both qhir1 and qhir8 was used from BC1F1 to BC1F4. Evaluation of the candidate individuals in each generation was carried out by pollinating to the tester of ZD958. Individuals with fast and clear pigmentation of the crossing immature embryos, high number of haploids per ear, and high haploid induction rate were considered as candidates. Finally, three new haploid inducer lines (CS1, CS2, and CS3) were developed. The first two (CS1 and CS2) were from the CAU3/S23, with a haploid induction rate of 8.29%–13.25% and 11.54%–15.54%, respectively. Meanwhile, the CS3 was from the CAU5/S23. Its haploid induction rate was 8.14%–12.28%. In comparison with the donor haploid inducer lines, the 24-h purple embryo rates of the newly developed haploid inducer lines were improved by 10%–20%, with a ~90% accuracy for the identification of haploid immature embryos. These new haploid inducer lines will further improve the efficiency of doubled haploid breeding of maize.

scholarly journals Influence of different wheat and Imperata cylindrica genetic backgrounds on haploid induction efficiency in wheat doubled haploid breeding

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 195-200 ◽  
Author(s):  
S.A. Rather ◽  
H.K. Chaudhary ◽  
V. Kaila
Keyword(s):  
Bread Wheat ◽  
Doubled Haploid ◽  
Combining Ability ◽  
General Combining Ability ◽  
Imperata Cylindrica ◽  
Haploid Induction ◽  
Embryo Formation ◽  
Haploid Production ◽  
Formation Efficiency ◽  
Doubled Haploid Breeding

Four Indian and one Japanese accession of Imperata cylindrica were assessed for their influence upon haploid production in F<sub>1</sub> generations of 21 wheat crosses (winter &times; spring, spring &times; spring and winter &times; winter) to find an efficient pollen source for haploid induction, which would enhance doubled haploid breeding in bread wheat. The frequency of haploid induction was influenced differently by the wheat and the I. cylindrica genotypes, indicating both maternal and paternal genetic influence on haploid induction. The gene actions controlling the inheritance of&nbsp; haploid induction&nbsp; appeared to be non-additive. Haploid formation efficiency was closely associated with other haploid induction parameters, i.e. pseudoseed formation, embryo formation and haploid regeneration. Amongst wheat F<sub>1</sub> groups, spring &times; spring wheats exhibited the highest potential for haploid induction. General combining ability for haploid production was highest for the, I. cylindrica genotype Ic-Aru, native to the northeastern Himalayas, which appears as a potential pollen source for efficient haploid induction in bread wheat.

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