scholarly journals Dihydrogergotamine, but not naloxone, counteracts lithium as an inhibitor of glucose-induced insulin release in isolated rat islets in vitro

Diabetologia ◽  
1987 ◽  
Vol 30 (3) ◽  
pp. 183-187 ◽  
Author(s):  
T. Fontela ◽  
O. Garcia Hermida ◽  
J. G�mez-Acebo
Keyword(s):  
Insulin Release ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Isolated Rat

Low concentrations of interleukin-1 stimulate and high concentrations inhibit insulin release from isolated rat islets of Langerhans

1986 ◽  
Vol 113 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Giatgen A. Spinas ◽  
Thomas Mandrup-Poulsen ◽  
Jens Mølvig ◽  
Leif Bæk ◽  
Klaus Bendtzen ◽  
...  
Keyword(s):  
Insulin Release ◽  
Interleukin 1 ◽  
Insulin Biosynthesis ◽  
Rat Islets ◽  
Low Concentrations ◽  
Isolated Rat Islets ◽  
High Concentrations ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat

Abstract. Isolated rat islets were incubated either with crude, affinity-purified or recombinant human interleukin-1 for 1 to 6 days. A significant (20–60%) increase of insulin release was observed at low concentrations of all three interleukin-1-containing preparations. In contrast, higher concentrations dose-dependently inhibited the insulin release. The increased insulin secretion occurred at concentrations below those necessary to augment the mitogen response to phytohaemagglutinin of murine thymocytes in vitro. These doses (0.05-0.5 U/ml) correspond to 0.2-2 ng of recombinant interleukin-1 per ml, equal to approximately 0.01-0.1 pmol/ml. In doses of 0.6-1.8 U/ml affinitypurified interleukin-1 significantly increased the islet insulin content per ng of DNA, indicating a stimulation of insulin-biosynthesis. The data support the concept that low concentrations of interleukin-1 may play a role in priming the physiological secretion of insulin.

scholarly journals Pentitols and insulin release by isolated rat islets of Langerhans

1968 ◽  
Vol 109 (3) ◽  
pp. 333-339 ◽  
Author(s):  
W Montague ◽  
K W Taylor
Keyword(s):  
Insulin Release ◽  
Islets Of Langerhans ◽  
Pentose Phosphate ◽  
Rat Islets ◽  
Isolated Islets Of Langerhans ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Stimulate Insulin Release ◽  
Isolated Rat

1. Insulin secretion was studied in isolated islets of Langerhans obtained by collagenase digestion of rat pancreas. In addition to responding to glucose and mannose as do whole pancreas and pancreas slices in vitro, isolated rat islets also secrete insulin in response to xylitol, ribitol and ribose, but not to sorbitol, mannitol, arabitol, xylose or arabinose. 2. Xylitol and ribitol readily reduce NAD+ when added to a preparation of ultrasonically treated islets. 3. Adrenaline (1μm) inhibits the effects of glucose and xylitol on insulin release. Mannoheptulose and 2-deoxy-glucose, however, inhibit the response to glucose but not that to xylitol. 4. The intracellular concentration of glucose 6-phosphate is increased when islets are incubated with glucose but not with xylitol, suggesting that xylitol does not promote insulin release by conversion into glucose 6-phosphate. 5. Theophylline (5mm) potentiates the effect of 20mm-glucose on insulin release from isolated rat islets of Langerhans, but has no effect on xylitol-mediated release. These results indicate that xylitol does not stimulate insulin release by alterations in the intracellular concentrations of cyclic AMP. 6. A possible role for the metabolism of hexoses via the pentose phosphate pathway in the stimulation of insulin release is discussed.

Alloxan Stimulation and Inhibition of Insulin Release from Isolated Rat Islets of Langerhans

Diabetes ◽  
1978 ◽  
Vol 27 (12) ◽  
pp. 1205-1214 ◽  
Author(s):  
D. C. Weaver ◽  
M. L. McDaniel ◽  
S. P. Naber ◽  
C. D. Barry ◽  
P. E. Lacy
Keyword(s):  
Insulin Release ◽  
Islets Of Langerhans ◽  
Rat Islets ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat
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