Physical mapping of differences in chloroplast DNA of the five wild-type plastomes in Oenothera subsection Euoenothera

1982 ◽  
Vol 61 (4) ◽  
pp. 373-384 ◽  
Author(s):  
K. H. J. Gordon ◽  
E. J. Crouse ◽  
H. J. Bohnert ◽  
R. G. Herrmann
Keyword(s):  
Chloroplast Dna ◽  
Physical Mapping ◽  
Wild Type

A new wild type of Citron (Citrus medica L., Rutaceae) identified through morphology and psbM-trnD spacer region of chloroplast DNA

Trees ◽  
2014 ◽  
Vol 28 (4) ◽  
pp. 1115-1124 ◽  
Author(s):  
Susheel Kumar ◽  
S. K. Malik ◽  
Ajit Uchoi ◽  
Rekha Chaudhury ◽  
S. R. Bhat
Keyword(s):  
Chloroplast Dna ◽  
Wild Type ◽  
Citrus Medica

Physical mapping of genes for chloroplast DNA encoded subunit polypeptides of the ATPsynthase complex from Petunia hybrida

1984 ◽  
Vol 8 (4) ◽  
pp. 283-290 ◽  
Author(s):  
A. Bovenberg ◽  
C. J. Howe ◽  
A. J. Kool ◽  
H. J. J. Nijkamp
Keyword(s):  
Chloroplast Dna ◽  
Physical Mapping ◽  
Petunia Hybrida

Pigment mutants of Chlamydomonas with deformed plastids and increased levels of chloroplast nucleic acids

1980 ◽  
Vol 42 (1) ◽  
pp. 127-127
Author(s):  
R.E. Wilson ◽  
H. Swift ◽  
K.S. Chiang
Keyword(s):  
Chloroplast Dna ◽  
Ribosomal Rna ◽  
Chloroplast Ultrastructure ◽  
Plastid Dna ◽  
Direct Consequence ◽  
Starch Metabolism ◽  
Wild Type ◽  
Rdna Genes ◽  
Chlorophyll A And B ◽  
Biochemical Analyses

The chloroplast ultrastructure of two Chlamydomonas reinhardtii pigment variant mutants, U3N and U3A, is strikingly different from that of the wild type. The mutant chloroplast has greatly lowered levels of chlorophyll a and b, and lacks the usual ordered thylakoid membrane structure. The amount of chloroplast ribosomes is increased, but the pyrenoid and surrounding starch grains appear to be unaltered. Our biochemical analyses have shown that, while the properties of chloroplast DNA, ribosomal RNA, and ribosomes in these mutants appeared to be normal, their relative amounts per cell increased markedly when compared to the wild type. In U3N these increases were approximately 60% for chloroplast DNA and 80% for chloroplast ribosomes. However, the ratio of chloroplast rDNA genes to total chloroplast DNA remained unchanged as shown by DNA-rRNA hybrdization. We propose that (1) The enhanced level of chloroplast ribosomes in these mutants is a direct consequence of the elevated amount of chloroplast DNA. Both of these increases may, in turn, arise from defective mechanism for their control. (2) These mutants grow successfully in the absence of functional photosynthesis, provided an external carbon source is available to them, but functional plastid DNA, ribosomes and protein synthesis may still be a requirement for normal starch metabolism.

Variegation in Selaginella martensii f. albovariegata. I. Expression and inheritance

1982 ◽  
Vol 60 (11) ◽  
pp. 2375-2383 ◽  
Author(s):  
James A. Tanno ◽  
Terry R. Webster
Keyword(s):  
Cell Division ◽  
Chloroplast Dna ◽  
Maternal Inheritance ◽  
Leaf Growth ◽  
Green Growth ◽  
Wild Type ◽  
Reciprocal Crosses ◽  
Leaf Variegation ◽  
White Tissue ◽  
Stable Leaf

Selaginella martensii f. albovariegata is a variegated sport which produces distinctly white tissue in an irregular fashion. Typical specimens possess not only variegated branches but some branches exhibiting uniformly green growth and others showing completely white growth. Variegated and green branches often change in character as they grow, while white branches are stable. Leaf variegation patterns are highly variable and strongly influenced by the cell division patterns of early leaf growth. Reciprocal crosses between wild-type S. martensii and f. albovariegata show maternal inheritance of variegation, suggesting cytoplasmic control. Further crosses, involving progeny of selected reciprocal crosses, also indicate a lack of direct nuclear influence on variegation. The details of character expression and inheritance can be accounted for on the basis of a random sorting of normal and defective cytoplasmic factors at cell division. Recent characterizations of chloroplast DNA for several species of green plants suggest that the DNA molecules within the plastid may represent the sorting factor.

scholarly journals CLONING AND PHYSICAL MAPPING OF AN EARLY REGION OF THE BACTERIOPHAGE T4 GENOME

Genetics ◽  
1984 ◽  
Vol 106 (1) ◽  
pp. 1-16
Author(s):  
Paul M Macdonald ◽  
Gisela Mosig
Keyword(s):  
Physical Mapping ◽  
Bacteriophage T4 ◽  
Restriction Endonucleases ◽  
Restriction Map ◽  
Wild Type ◽  
End Points ◽  
Restriction Fragments ◽  
Early Region ◽  
Dna Restriction Fragments ◽  
Dna Restriction

ABSTRACT We have cloned DNA restriction fragments from the largely nonessential region of bacteriophage T4 located between genes 39 and 56. The cloned DNA fragments were used to construct a precise map of the sites in this region recognized by eight restriction endonucleases. This restriction map allowed us to compare the cytosine-containing T4 DNA used for cloning with the hydroxymethylcytosine-containing DNA of wild-type T4; there were no detectable rearrangements in the region tested. We were also able to determine the physical locations of several deletion end points and of several genes.

Physical mapping of 4S RNA genes on chloroplast DNA of Spirodela oligorhiza

1981 ◽  
Vol 4 (3) ◽  
pp. 187-190 ◽  
Author(s):  
Gert S. P. Groot ◽  
Nel van Harten-Loosbroek
Keyword(s):  
Chloroplast Dna ◽  
Physical Mapping ◽  
Spirodela Oligorhiza ◽  
Rna Genes

scholarly journals Use of the fluorochrome 4'6-diamidino-2-phenylindole in genetic and developmental studies of chloroplast DNA.

1979 ◽  
Vol 82 (1) ◽  
pp. 299-305 ◽  
Author(s):  
A W Coleman
Keyword(s):  
Chloroplast Dna ◽  
Marine Algae ◽  
Developmental Changes ◽  
Developmental Studies ◽  
Wild Type ◽  
Peripheral Ring ◽  
Structural Plan ◽  
Chromophyte Algae ◽  
Dapi Fluorescence

Use of the DNA-specific fluorochrome 4'6-diamidino-2-phenylindole (DAPI) makes it possible to examine in situ the structure of chloroplast DNA (chDNA) with the fluorescence microscope. This simplifies the study of genetic and developmental changes in chloroplast DNA. Three examples are presented. (a) Wild-type Euglena gracilis B contains several chloroplast DNA nucleoids per chloroplast. A yellow mutant lacking functional chloroplasts is similar, but such nucleoids are absent in an aplastidic mutant strain known from biochemical studies to have lost its chDNA. (b) In vegetative cells of the giant-celled marine algae Acetabularia and Batophora, only about a quarter of the chloroplasts have even one discernible chloroplast DNA particle, and such particles vary in size, showing a 30-fold variation in the amount of DNA-bound DAPI fluorescence detected per chloroplast. By contrast, 98% of chloroplasts in developing Acetabularia cysts contain chDNA, with as many as nine nucleoids per chloroplast. (c) DAPI-stained chloroplasts of chromophyte algae display the peripheral ring of DNA expected from electron microscope studies. However, these rings are not uniform in thickness, but are necklace-like, with the appearance of beads on a string. Since the multiple nucleoids in plastids of chlorophyte algae also appear to be interconnected throughout the chloroplast, a common structural plan may underlie chDNA morphology in both groups of algae.

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