High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: Isolation of strains with suppressor-enhancing mutations

1980 ◽  
Vol 177 (4) ◽  
pp. 545-552 ◽  
Author(s):  
Max P. Oeschger ◽  
Nicole S. Oeschger ◽  
Grant T. Wiprud ◽  
Sharon Lee Woods
Keyword(s):  
Escherichia Coli ◽  
High Efficiency ◽  
Temperature Sensitive ◽  
Escherichia Coli K12 ◽  
Amber Suppressor

An amber mutation in the gene encoding the beta' subunit of Escherichia coli RNA polymerase

1982 ◽  
Vol 152 (2) ◽  
pp. 736-746
Author(s):  
S P Ridley ◽  
M P Oeschger
Keyword(s):  
Escherichia Coli ◽  
High Temperature ◽  
Rna Polymerase ◽  
Coli Strain ◽  
Beta Subunit ◽  
Temperature Sensitive ◽  
Amber Mutation ◽  
Gene Encoding ◽  
Amber Suppressor

An Escherichia coli strain carrying an amber mutation (UAG) in rpoC, the gene encoding the beta prime subunit of RNA polymerase, was isolated after mutagenesis with nitrosoguanidine. The mutation was moved into an unmutagenized strain carrying the supD43,74 allele, which encodes a temperature-sensitive su1 amber suppressor, and sue alleles, which enhance the efficiency of the suppressor. In this background, beta prime is not synthesized at high temperature. Suppression of the mutation by the non-temperature-sensitive amber suppressor su1+ yields a protein which is functional at all temperatures examined (30, 37, and 42 degrees C).

scholarly journals Cell Envelope and Shape of Escherichia coli K12 Properties of a Temperature-Sensitive rod Mutant

1972 ◽  
Vol 26 (4) ◽  
pp. 570-586 ◽  
Author(s):  
Ulf Henning ◽  
Kurt Rehn ◽  
Barbara Hohn ◽  
Volkmar Braun ◽  
Uli Sschwar
Keyword(s):  
Escherichia Coli ◽  
Cell Envelope ◽  
Temperature Sensitive ◽  
Escherichia Coli K12
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