Complement-activating properties of immune complexes are suppressed by IgM rheumatoid factor and enhanced by IgG rheumatoid factor

1988 ◽  
Vol 8 (3) ◽  
pp. 95-100 ◽  
Author(s):  
M. Shingu ◽  
I. Ezaki ◽  
M. Nobunaga
Keyword(s):  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Igm Rheumatoid Factor ◽  
Igg Rheumatoid Factor

IgM rheumatoid factor amplifies the inflammatory response of macrophages induced by the rheumatoid arthritis-specific immune complexes containing anticitrullinated protein antibodies

2014 ◽  
Vol 74 (7) ◽  
pp. 1425-1431 ◽  
Author(s):  
Lætitia Laurent ◽  
Florence Anquetil ◽  
Cyril Clavel ◽  
Ndiémé Ndongo-Thiam ◽  
Géraldine Offer ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Cytokine Response ◽  
Igm Rheumatoid Factor ◽  
Tnf Α ◽  
Vitro Model ◽  
Synovial Membranes ◽  
Anticitrullinated Protein Antibodies

ObjectivesAnticitrullinated protein antibodies (ACPA) are specifically associated with rheumatoid arthritis (RA) and produced in inflamed synovial membranes where citrullinated fibrin, their antigenic target, is abundant. We showed that immune complexes containing IgG ACPA (ACPA-IC) induce FcγR-mediated tumour necrosis factor (TNF)-α secretion in macrophages. Since IgM rheumatoid factor (RF), an autoantibody directed to the Fc fragment of IgG, is also produced and concentrated in the rheumatoid synovial tissue, we evaluated its influence on macrophage stimulation by ACPA-IC.MethodsWith monocyte-derived macrophages from more than 40 healthy individuals and different human IgM cryoglobulins with RF activity, using a previously developed human in vitro model, we evaluated the effect of the incorporation of IgM RF into ACPA-IC.ResultsIgM RF induced an important amplification of the TNF-α secretion. This effect was not observed in monocytes and depended on an increase in the number of IgG-engaged FcγR. It extended to the secretion of interleukin (IL)-1β and IL-6, was paralleled by IL-8 secretion and was not associated with overwhelming secretion of IL-10 or IL-1Ra. Moreover, the RF-induced increased proinflammatory bioactivity of the cytokine response to ACPA-IC was confirmed by an enhanced, not entirely TNF-dependent, capacity of the secreted cytokine cocktail to prompt IL-6 secretion by RA synoviocytes.ConclusionsBy showing that it can greatly enhance the proinflammatory cytokine response induced in macrophages by the RA-specific ACPA-IC, these results highlight a previously undescribed, FcγR-dependent strong proinflammatory potential of IgM RF. They clarify the pathophysiological link between the presence of ACPA and IgM RF, and RA severity.

scholarly journals Cytokine profile of macrophages in vitro stimulated by ACPA immune complexes in the presence or absence of IgM rheumatoid factor

2011 ◽  
Vol 70 (Suppl 2) ◽  
pp. A38-A38
Author(s):  
L. Laurent ◽  
C. Clavel ◽  
F. Anquetil ◽  
J.-L. Pasquali ◽  
M. Sebbag ◽  
...  
Keyword(s):  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Cytokine Profile ◽  
Igm Rheumatoid Factor

scholarly journals Separation and characterization of immune complexes containing 19S IgM rheumatoid factor-IgG in juvenile arthritis

1983 ◽  
Vol 26 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Terry L. Moore ◽  
P. Wayne Sheridan ◽  
Jack Zuckner ◽  
Robert W. Dorner
Keyword(s):  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Juvenile Arthritis ◽  
Igm Rheumatoid Factor
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