Cis-trans position effect in Salmonella phage P22

1972 ◽  
Vol 115 (1) ◽  
pp. 19-25
Author(s):  
R. Kolstad ◽  
L. Enquist
Keyword(s):  
Position Effect ◽  
Trans Position ◽  
Phage P22 ◽  
Salmonella Phage

scholarly journals Maturation of the tail spike endorhamnosidase of Salmonella phage P22.

1982 ◽  
Vol 257 (13) ◽  
pp. 7864-7871 ◽  
Author(s):  
D P Goldenberg ◽  
P B Berget ◽  
J King
Keyword(s):  
Phage P22 ◽  
Tail Spike ◽  
Salmonella Phage

scholarly journals A Salmonella Phage-P22 Mutant Defective in Abortive Transduction

Genetics ◽  
1997 ◽  
Vol 145 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Nicholas R Benson ◽  
John Roth
Keyword(s):  
Protein S ◽  
Lytic Infection ◽  
Genetic Element ◽  
Bacterial Dna ◽  
Phage Mutant ◽  
Viral Particles ◽  
Phage Dna ◽  
Phage P22 ◽  
Mutant Locus ◽  
Salmonella Phage

In the course of a lytic infection the Salmonella phage P22 occasionally encapsulates bacterial DNA instead of phage DNA. Thus, phage lysates include two classes of viral particles. Phage particles carrying bacterial DNA are referred to as transducing particles and deliver this DNA to a host as efficiently as particles carrying phage DNA. Once injected, the transduced DNA can either recombine with the recipient chromosome to form a “complete” transductant, or it can establish itself as an expressible, nonreplicating genetic element and form an “abortive” transductant. In this work, we describe a P22-phage mutant with reduced ability to form abortive transductants. The mutation responsible for this phenotype, called tdx-1, was found as one of two mutations contributing to the high-transducing phenotype of the P22-mutant HT12/4. In addition, the tdx-1 mutation is lethal when combined with an erf-am mutation. The tdx-1 mutation has been mapped to a region of the P22 genome that encodes several injected proteins and may involve more than one mutant locus. The phenotypes of the tdx-1 mutation suggest that the Tdx protein(s) normally assist in the circularization of the P22 genome and also contribute to the formation of DNA circles thought to be required for abortive transduction.

Phage genes involved in the formation of generalized transducing particles in Salmonella-phage P22

1974 ◽  
Vol 135 (2) ◽  
pp. 175-184 ◽  
Author(s):  
A. Sudharsan Raj ◽  
A. Yesoda Raj ◽  
Horst Schmieger
Keyword(s):  
Phage P22 ◽  
Salmonella Phage

scholarly journals REGIONAL SPECIFICITY OF ILLEGITIMATE RECOMBINATION BY THE TRANSLOCATABLE AMPLICILLIN-RESISTANCE ELEMENT Tn1 IN THE GENOME OF PHAGE P22

Genetics ◽  
1979 ◽  
Vol 92 (3) ◽  
pp. 685-710
Author(s):  
George M Weinstock ◽  
Miriam M Susskind ◽  
David Botstein
Keyword(s):  
Electron Microscopy ◽  
Gene Function ◽  
The Other ◽  
Illegitimate Recombination ◽  
Physical Analysis ◽  
Irreversible Loss ◽  
Ampicillin Resistance ◽  
Regional Specificity ◽  
Phage P22 ◽  
Salmonella Phage

ABSTRACT Insertions of the translocatable ampicillin-resistance element Tnl were selected in the genome of the temperate Salmonella phage P22 by growing the phage on hosts carrying the resistance plasmid RP4. Insertions of Tnl into phage P22 are rare (10-10 per phage) and nonrandomly distributed in the P22 genome. They are found mainly in the vicinity of the P22 ant gene. Insertions within the ant gene are found at many (at least 15) genetically separable sites, are found equally frequently in both orientations and cause irreversible loss of gene function. Some insertions in ant appear to be associated with an adjecent deletion.—Prophage deletions were derived from P22::Tnl phages by two methods. Low multiplicity transductants have nonrandomly distributed endpoints. One end is at or very near the site of the Tnl insertion, and the other is in the vicinity of gene 12; however, there are many genetically distinguishable endpoints within gene 12. Prophage deletions selected as survivors of induction of a P22Ap mnt-ts lysogen have similarly nonrandom endpoints, with the Tnl-distal end frequently near the ant gene, as well as gene 12. Physical analysis of several prophage deletions suggests that the Tnl is intact to the resolution of DNA electron microscopy and that the deletions begin at the end of the Tnl insertion.—These results suggest that illegitimate recombination associated with Tnl shows regional specificity (i.e., preference for some large areas of the P22 genome over other areas), but that within these regions is quite nonspecific.

scholarly journals Genomic structure of phage F22, a hybrid between serologically and morphologically unrelatedSalmonella typhimuriumbacteriophages P22 andFels 2

1986 ◽  
Vol 48 (3) ◽  
pp. 139-143 ◽  
Author(s):  
Nobuto Yamamoto ◽  
Robert J. McDonald
Keyword(s):  
Salmonella Typhimurium ◽  
Genomic Structure ◽  
Homologous Region ◽  
Structural Genes ◽  
Marker Rescue ◽  
Early Genes ◽  
Late Genes ◽  
Protein Coat ◽  
Phage P22 ◽  
Salmonella Phage

SummarySalmonella typhimuriumphage P22 can recombine with a serologically and morphologically unrelated Salmonella phageFels 2to yield the hybrid phage F22 at a frequency of about 10−12. F22 has inherited the entire late genes (protein coat and tail structural genes) ofFels 2but carries some P22 early genes. P22 genes in the F22 phage were indentified by marker rescue of various P22 mutants. The F22 genome carries thex-erf-c-18-12segment of the P22 genome. A number of P22 amber mutants were also tested to support these data. The F22 region homologous to P22 was mapped by scoring the ratio of P22 backcross recombinant types in lysates of F22 lysogens superinfected with P22c2ts12. The ratio of the distances between these markers and the ends of the homologous region was determined. Furthermore a new F22 hybrid designated F22dis, containing both P22 immunity regions (candIm), was isolated at a frequency of about 10−4by superinfecting F22 lysogens with P22. F22disphage has lost someFels 2gene(s) which have been replaced by the P22 segment containing theImregion, resulting in formation of a defective hybrid phage.

λimmP22dis: A hybrid of coliphage λ with both immunity regions of Salmonella phage P22

1978 ◽  
Vol 166 (3) ◽  
pp. 233-243 ◽  
Author(s):  
Nobuto Yamamoto ◽  
J. A. Wohlhieter ◽  
P. Gemski ◽  
L. S. Baron
Keyword(s):  
Phage P22 ◽  
Salmonella Phage

The immI region of Salmonella phage P22

1984 ◽  
Vol 198 (1) ◽  
pp. 105-109 ◽  
Author(s):  
H. H. Prell ◽  
J. Auer ◽  
J. Soška ◽  
P. Hava
Keyword(s):  
Phage P22 ◽  
Salmonella Phage

Effects of P32 decay on transduction by Salmonella phage P22

Virology ◽  
1962 ◽  
Vol 17 (2) ◽  
pp. 233-244 ◽  
Author(s):  
Philip E. Hartman ◽  
A.W. Kozinski
Keyword(s):  
Phage P22 ◽  
Salmonella Phage
Sign in / Sign up

Export Citation Format

Share Document