A novel mutant of bacteriophage T7 that is defective in early phage DNA synthesis

1980 ◽  
Vol 179 (3) ◽  
pp. 683-691 ◽  
Author(s):  
Richard North ◽  
Ian J. Molineux
Keyword(s):  
Dna Synthesis ◽  
Bacteriophage T7 ◽  
Phage Dna

Development of T7 phage and T7 phage containing apurinic sites in an exonuclease III, endonuclease IV double mutant of Escherichia coli

1992 ◽  
Vol 70 (7) ◽  
pp. 605-608 ◽  
Author(s):  
Giselle Sanchez ◽  
Margaret D. Mamet-Bratley
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Double Mutant ◽  
Bacteriophage T7 ◽  
Bacterial Dna ◽  
Exonuclease Iii ◽  
Repair Enzymes ◽  
Phage Dna ◽  
T7 Phage

The development of bacteriophage T7 was examined in an Escherichia coli double mutant defective for the two major apurinic, apyrimidinic endonucleases (exonuclease III and endonuclease IV, xth nfo). In cells infected with phages containing apurinic sites, the defect in repair enzymes led to a decrease of phage survival and a total absence of bacterial DNA degradation and of phage DNA synthesis. These results directly demonstrate the toxic action of apurinic sites on bacteriophage T7 at the intracellular level and its alleviation by DNA repair. In addition, untreated T7 phage unexpectedly displayed reduced plating efficiency and decreased DNA synthesis in the xth nfo double mutant.Key words: apurinic sites, DNA repair, T7 phage.

scholarly journals Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers

2017 ◽  
Vol 114 (12) ◽  
pp. E2310-E2318 ◽  
Author(s):  
Bin Zhu ◽  
Longfei Wang ◽  
Hitoshi Mitsunobu ◽  
Xueling Lu ◽  
Alfredo J. Hernandez ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deep Sea ◽  
Phage Dna

scholarly journals Mutagenesis inEscherichia coli

1970 ◽  
Vol 15 (2) ◽  
pp. 147-156 ◽  
Author(s):  
B. A. Bridges ◽  
Rachel E. Dennis ◽  
R. J. Munson
Keyword(s):  
Dna Synthesis ◽  
Mutation Rate ◽  
Phage Genome ◽  
Multiplicity Of Infection ◽  
Mutation Process ◽  
Amber Mutation ◽  
Induced Mutation ◽  
Phage Dna ◽  
T4 Phage ◽  
Image Position

SUMMARYA system has been developed for the study of reversion of an amber mutation responsible for a deficiency in DNA synthesis in T4 phage E51. When complexed with bacteria able to suppress the amber mutation the induced mutation rate per phage genome per rad isWhen complexed with bacteria unable to suppress the amber mutation (and being thus unable to synthesize phage DNA) the induced mutation rate is at least 14 times lower indicating that DNA synthesis is necessary for the production of the majority of functional reversions at the amber site. The induced mutation rate in suppressor-containing bacteria is independent of multiplicity of infection between 0·2 and 5, suggesting that recombination immediately after irradiation between phage genomes is unlikely to be a requirement for the mutation process.

Studies on bacteriophage T7 DNA synthesis in vitro

1975 ◽  
Vol 141 (3) ◽  
pp. 233-249 ◽  
Author(s):  
Eberhard Scherzinger ◽  
Günther Klotz
Keyword(s):  
Dna Synthesis ◽  
Bacteriophage T7

scholarly journals Phage DNA synthesis and host DNA degradation in the life cycle of Lactococcus lactis bacteriophage c6A

1992 ◽  
Vol 138 (5) ◽  
pp. 945-950 ◽  
Author(s):  
I. B. Powell ◽  
D. L. Tulloch ◽  
A. J. Hillier ◽  
B. E. Davidson
Keyword(s):  
Life Cycle ◽  
Dna Synthesis ◽  
Lactococcus Lactis ◽  
Dna Degradation ◽  
Phage Dna

The Synthesis of Nucleic Acids in Bacillus subtilis Infected with Phage PBS 1

1973 ◽  
Vol 51 (9) ◽  
pp. 1219-1224 ◽  
Author(s):  
B. K. Rima ◽  
I. Takahashi
Keyword(s):  
Bacillus Subtilis ◽  
Nucleic Acids ◽  
Dna Synthesis ◽  
Trichloroacetic Acid ◽  
Actinomycin D ◽  
The Other ◽  
Phage Dna ◽  
Infected Cells ◽  
Phage Growth ◽  
A New Species

Unlike other phage systems, the development of PBS 1 was found to be insensitive to rifamycin SV. The incorporation of 3H-uridine into trichloroacetic acid precipitable and alkali-labile material (RNA), in PBS-1-infected cells, was greatly reduced by rifamycin. Observations that RNA synthesized in the presence of rifamycin was hybridizable exclusively with the phage DNA and that actinomycin D inhibited the phage growth indicated that the synthesis of a new species of RNA was required for the development of PBS 1. The host DNA synthesis was reduced to a very low level 5 min after infection. The phage DNA synthesis was also apparently reduced markedly by rifamycin when determined with 3H-uridine as labelling material. On the other hand, rifamycin did not affect the incorporation of 3H-deoxycytidine into the phage DNA, suggesting that phage DNA synthesis was in fact insensitive to rifamycin. It is not clear how rifamycin inhibits the incorporation of 3H-uridine into nucleic acids in PBS-1-infected cells.

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