Excision of the 40kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats

1981 ◽  
Vol 184 (1) ◽  
pp. 97-101 ◽  
Author(s):  
Pierre Meulien ◽  
Robert G. Downing ◽  
Paul Broda
Keyword(s):  
Pseudomonas Putida ◽  
Direct Repeats ◽  
Tol Plasmid

scholarly journals Conjugal TOL Transfer from Pseudomonas putida to Pseudomonas aeruginosa: Effects of Restriction Proficiency, Toxicant Exposure, Cell Density Ratios, and Conjugation Detection Method on Observed Transfer Efficiencies

2005 ◽  
Vol 71 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Catalina Arango Pinedo ◽  
Barth F. Smets
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Strong Dependence ◽  
Plasmid Transfer ◽  
Mass Action ◽  
Tol Plasmid ◽  
Toxicant Exposure ◽  
Transfer Frequency ◽  
Cell Densities

ABSTRACT The effects of restriction proficiency and premating exposure to toxicants on conjugal transfer of the TOL plasmid between Pseudomonas spp. was investigated by examinations of filter matings. A Pseudomonas putida KT2442-derived strain carrying a gfp-tagged variant of the TOL plasmid was used as a donor, and both restriction-deficient (PAO1162N) and -proficient (PAO2002N) Pseudomonas aeruginosa strains were used as recipients. The in situ enumeration of conjugation events allowed us to obtain frequency estimates that were unbiased by transconjugant growth or plasmid retransfer. We observed a strong dependence of the plasmid transfer frequency on the initial donor-to-recipient ratio of surface matings, which invalidated the use of mass action-based plasmid transfer kinetic estimators. Careful control of the initial parental cell densities permitted evaluations of the true effects of restriction proficiency and toxicant exposure on TOL transfer. At standard donor-to-recipient ratios (10−3 for PAO1162N and 2 � 101 for PAO2002N) and total cell densities (105 cells/mm2 for PAO1162N and 106 cells/mm2 for PAO2002N), plasmid transfer frequencies without toxicant exposure were approximately 10−7 (events/mm2)−1 for PAO1162N and 10−11 (events/mm2)−1 for PAO2002N based on in situ observations of conjugation events. The enumeration of transconjugants via selective plating yielded transfer frequencies that were up to 1 order of magnitude lower. Premating exposure to sodium dodecyl sulfate (1 to 10 mM) significantly increased the transfer frequency for the restriction-proficient strain PAO2002N (P < 0.05) but not for the restriction-deficient strain PAO1162N. On the other hand, premating exposure to ethanol, toluene, or phenol had no positive effect on the plasmid transfer frequency. Clearly, restriction proficiency provides a strong barrier to interspecific transfer of the TOL plasmid, and this barrier was only marginally attenuated by recipient exposure to toxicants within the ranges examined.

scholarly journals Identification of chromosomally integrated TOL DNA in cured derivatives of Pseudomonas putida PAW1

1982 ◽  
Vol 152 (2) ◽  
pp. 911-914
Author(s):  
P Meulien ◽  
P Broda
Keyword(s):  
Pseudomonas Putida ◽  
Parental Strain ◽  
Chromosomal Dna ◽  
Tol Plasmid ◽  
Derivatives Of

Some plasmid-free Tol- strains derived from Pseudomonas putida PAW1 (which carries the TOL plasmid pWW0) have a segment of TOL DNA located chromosomally. Of three independently isolated strains, PAW86 had an integrated TOL segment of 16 kilobases and PAW85 had two copies of this segment in different chromosomal locations, whereas the chromosomal DNA of PAW82 showed no homology with the TOL plasmid. In cultures of the parental strain, it appears that a 56-kilobase TOL DNA segment is located chromosomally in some cells.

Organisation of the tmb catabolic operons of Pseudomonas putida TMB and evolutionary relationship with the xyl operons of the TOL plasmid pWW0

Gene ◽  
1996 ◽  
Vol 182 (1-2) ◽  
pp. 189-193 ◽  
Author(s):  
Rebecca Favaro ◽  
Camilla Bernasconi ◽  
Nadia Passini ◽  
Giovanni Bertoni ◽  
Giuseppina Bestetti ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Evolutionary Relationship ◽  
Tol Plasmid
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