Vicia faba chloroplast DNA has only one set of ribosomal RNA genes as shown by partial denaturation mapping and R-loop analysis

1980 ◽  
Vol 178 (2) ◽  
pp. 261-269 ◽  
Author(s):  
Barbara Koller ◽  
Hajo Delius
Keyword(s):  
Chloroplast Dna ◽  
Vicia Faba ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Loop Analysis ◽  
Partial Denaturation ◽  
Rna Genes

scholarly journals Heritability and Variability in Ribosomal RNA Genes of Vicia faba

Genetics ◽  
1987 ◽  
Vol 117 (2) ◽  
pp. 285-295
Author(s):  
Scott O Rogers ◽  
Arnold J Bendich
Keyword(s):  
Vicia Faba ◽  
Ribosomal Rna ◽  
Copy Number ◽  
Nearest Neighbor ◽  
Blot Hybridization ◽  
Ribosomal Rna Genes ◽  
Hybridization Pattern ◽  
Nontranscribed Spacer ◽  
Rna Genes ◽  
Eco Ri

ABSTRACT We have compared the restriction patterns and copy numbers of ribosomal RNA genes (rDNA) between and within individuals of Vicia faba. While the Eco RI blot-hybridization patterns changed only after one to two generations, copy number changes were found among different tissues of the same plant. Copy number differences among individuals in the population were as great as 95-fold, whereas as much as a 12-fold variation was seen among tissues of the same plant. Among individual F1 progeny from genetic crosses, nearly an 8-fold variation was seen, and among individuals of the F2 generation a spread of 22-fold was measured. Among individual siblings of self-pollinated parents, up to 7-fold variation was observed. However, changes in copy number did not necessarily indicate changes in rDNA Eco RI blot-hybridization pattern, and vice versa. Furthermore, nearest neighbor analysis of R-loop experiments showed that the arrangement of members of the "nontranscribed" spacer (NTS) size classes along the chromosome was not random, but some clustering was indicated. The data are consistent with the hypothesis that sister chromatid exchange in somatic cells of V. faba is the primary mechanism for altering the rDNA copy number as well as causing the extreme variation observed in the NTS. Variation among individuals in rDNA blot-hybridization pattern was also observed for Vicia villosa, Vicia dasycarpa, Vicia benghalensis and Vicia pannonica.

Variation in the ribosomal RNA genes among individuals of Vicia faba

1986 ◽  
Vol 6 (5) ◽  
pp. 339-345 ◽  
Author(s):  
Scott O. Rogers ◽  
Sandra Honda ◽  
Arnold J. Bendich
Keyword(s):  
Vicia Faba ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Rna Genes

scholarly journals Mapping of the ribosomal RNA genes on spinach chloroplast DNA

1978 ◽  
Vol 5 (6) ◽  
pp. 1741-1751 ◽  
Author(s):  
P.R. Whitfeld ◽  
R.G. Herrmann ◽  
W. Bottomley
Keyword(s):  
Chloroplast Dna ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Spinach Chloroplast ◽  
Rna Genes
Sign in / Sign up

Export Citation Format

Share Document