Somatic hybrid plants from sexually incompatible woody species: Citrus reticulata and Citropsis gilletiana

1990 ◽  
Vol 8 (11) ◽  
pp. 656-659 ◽  
Author(s):  
J. W. Grosser ◽  
F. G. Gmitter ◽  
N. Tusa ◽  
J. L. Chandler
Keyword(s):  
Somatic Hybrid ◽  
Woody Species ◽  
Citrus Reticulata ◽  
Hybrid Plants

scholarly journals SOMATIC HYBRIDIZATION BETWEEN CITRUS RETICULATA AND CITROPSIS GABUNENSIS THROUGH ELECTROFUSION

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1155d-1155
Author(s):  
Jinq-Tian Ling ◽  
N. Nito ◽  
M. Iwamasa
Keyword(s):  
Electric Fields ◽  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Morphological Characteristics ◽  
Chromosome Count ◽  
Citrus Reticulata ◽  
Embryogenic Calli ◽  
Isoenzyme Analysis ◽  
Hybrid Plants ◽  
Agar Selection

Protoplasts were isolated from embryogenic calli of Citrus reticulata cv. Ponkan and Citropsis gabunensis, and fused in electric fields. The maximal fusion efficiency was obtained by application of AC at 75 V/cm (1.0 MHz) and DC square-wave pulse at 1.125 KV/cm for 40 usec. Fusion-treated protoplasts were cultured on MT medium without phytohormone, solidified with 0.6% agar. Colonies from the protoplasts were proliferated on MT medium with zeatin 1 mg/l and 0.9% agar. Selection of somatic hybrid callus was based on chromosome count and isoenzyme analysis. The somatic hybrids were tetraploid (2n=36). C. reticulata and C. gabunensis were both homozygous at Got-1 locus, but distinguishable easily because band of the latter migrated faster than that of the former. In zymogram of somatic hybrid, both parent bands were retained and a new hybrid band was also evident between them. Embryos from somatic-hybrid callus regenerated intact plant. The hybrid plants showed intermediate morphological characteristics of the parents.

Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark

Plant Science ◽  
1988 ◽  
Vol 57 (3) ◽  
pp. 215-223 ◽  
Author(s):  
Darasinh Sihachakr ◽  
Robert Haicour ◽  
Isabelle Serraf ◽  
Edilberto Barrientos ◽  
Catherine Herbreteau ◽  
...  
Keyword(s):  
Somatic Hybrid ◽  
Solanum Melongena ◽  
Hybrid Plants ◽  
Solanum Khasianum

scholarly journals Production of Somatic Hybrid Plants between Japanese Bunching Onion(Allium fistulosum L.) and Bulb Onion(A. cepa L.) via Electrofusion.

2002 ◽  
Vol 71 (5) ◽  
pp. 623-631 ◽  
Author(s):  
Masahito Shimonaka ◽  
Takashi Hosoki ◽  
Motonori Tomita ◽  
Yoshimasa Yasumuro
Keyword(s):  
Somatic Hybrid ◽  
Allium Fistulosum ◽  
Bulb Onion ◽  
Hybrid Plants ◽  
Japanese Bunching Onion ◽  
Bunching Onion

Selection of Somatic Hybrid Plants in Nicotiana Through Fluorescence-Activated Sorting of Protoplasts

1985 ◽  
Vol 3 (9) ◽  
pp. 811-816 ◽  
Author(s):  
Claudio L. Afonso ◽  
Kristi R. Harkins ◽  
Mary A. Thomas-Compton ◽  
Ann E. Krejci ◽  
David W. Galbraith
Keyword(s):  
Somatic Hybrid ◽  
Hybrid Plants ◽  
Selection Of

scholarly journals Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

2000 ◽  
Vol 23 (3) ◽  
pp. 661-665 ◽  
Author(s):  
Fernanda Januzzi Mendes-da-Glória ◽  
Francisco de Assis Alves Mourão Filho ◽  
Luis Eduardo Aranha Camargo ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Somatic Hybrid ◽  
Leaf Morphology ◽  
Somatic Hybrids ◽  
Sweet Orange ◽  
Southeastern Brazil ◽  
Citrus Industry ◽  
Rangpur Lime ◽  
Rapd Profile ◽  
Hybrid Plants ◽  
Regenerated Plants

Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.

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