The relationship of nitrogen source and in vivo nitrate reductase actiity to root formation in Euphorbia esula cell suspension cultures

1988 ◽  
Vol 7 (5) ◽  
pp. 361-364 ◽  
Author(s):  
K. J. Evenson ◽  
D. S. Galitz ◽  
D. G. Davis
Keyword(s):  
Nitrate Reductase ◽  
Cell Suspension ◽  
Nitrogen Source ◽  
Root Formation ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Euphorbia Esula ◽  
Relationship Of ◽  
The Relationship

Über den Abbau von Flavonolen in pflanzlichen Zellsuspensionskulturen / Degradation of Flavonols in Plant Suspension Cultures

1972 ◽  
Vol 27 (8) ◽  
pp. 946-954 ◽  
Author(s):  
Wolfgang Hösel ◽  
Paul D. Shaw ◽  
Wolfgang Barz
Keyword(s):  
Salicylic Acid ◽  
Glycine Max ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Enzyme Preparations ◽  
Cicer Arietinum L

The flavonols kaempferol, quercetin and isorhamnetin were labelled with 14C by keeping seven day old Cicer arietinum L. plants in an atmosphere of 14CO2 for five days. The purified (U-14C) flavonols were applied to cell suspension cultures of Cicer arietinum L., Phaseolus aureus Roxb., Glycine max and Petroselinum hortense. Based on the rates of 14CO2 formation and distribution of radioactivity after fractionation of the cells, the flavonols were shown to be catabolized to a very high extent.All four cell suspension cultures possess the enzymatic activity transforming flavonols to the recently discovered 2,3-dihydroxyflavanones. Upon incubation of the flavonols datiscetin and kaempferol with enzyme preparations from Cicer arietinum L. cell suspension cultures, it was demonstrated that the enzymatically formed 2,3-dihydroxyflavanones are further transformed in an enzyme catalyzed reaction. Salicylic acid was found as a degradation fragment of ring B of the 2,3,5,7,2′-pentahydroxyflavanone derived from datiscetin. Neither phloroglucinol nor phloroglucinol carboxylic acid were observed as metabolites of ring A. These in vitro findings were further substantiated by in vivo data because the flavonols kaempferol, quercetin and datiscetin when applied to cell suspension cultures of Cicer arietinum L. and Glycine max gave rise to para-hydroxybenzoic acid, protocatechuic acid and salicylic acid, respectively. It was thus concluded that flavonols are catabolized via 2,3-dihydroxyflavanones with the B-ring liberated as the respective benzoic acid. The data are discussed in connection with earlier findings on the catabolism of chalcones, cinnamic and benzoic acids.

Oxidative Decarboxylation of para-Hydroxybenzoic Acids by Peroxidases under in vivo and in vitro Conditions

1975 ◽  
Vol 30 (9-10) ◽  
pp. 650-658 ◽  
Author(s):  
Jochen Berlin ◽  
Wolfgang Barz
Keyword(s):  
Cell Suspension ◽  
Specific Activity ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Oxidative Decarboxylation ◽  
Benzoic Acids ◽  
Plant Cell Suspension Cultures ◽  
Hydroxybenzoic Acids

Oxidative decarboxylation of p-hydroxybenzoic acids in plant cell suspension cultures is catalyzed by peroxidases. This reaction has been characterized in vivo and in vitro. Decarboxylation of substituted benzoic acids yields monomeric, dimeric and oligomeric benzoquinones. All peroxidases obtained from soybean (Glycine max) cell suspension cultures by gel electrophoresis are equally capable to decarboxylate p-hydroxybenzoic acids as indicated by their rather low differences in specific activity for various benzoic acids.

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