Temperature related alterations in the acidic alanine-rich “A” protein from the 50S ribosomal particle of the extreme halophile, Halobacterium cutirubrum

1975 ◽  
Vol 140 (1) ◽  
pp. 15-27 ◽  
Author(s):  
Arne Reider Strøm ◽  
George Oda ◽  
Sadiq Hasnain ◽  
Makoto Yaguchi ◽  
Louis Peter Visentin
Keyword(s):  
Extreme Halophile ◽  
Ribosomal Particle

scholarly journals Systems Analysis of Bioenergetics and Growth of the Extreme Halophile Halobacterium salinarum

2009 ◽  
Vol 5 (4) ◽  
pp. e1000332 ◽  
Author(s):  
Orland Gonzalez ◽  
Susanne Gronau ◽  
Friedhelm Pfeiffer ◽  
Eduardo Mendoza ◽  
Ralf Zimmer ◽  
...  
Keyword(s):  
Systems Analysis ◽  
Halobacterium Salinarum ◽  
Extreme Halophile

scholarly journals The Vibrio harveyi GTPase CgtAV Is Essential and Is Associated with the 50S Ribosomal Subunit

2006 ◽  
Vol 188 (3) ◽  
pp. 1205-1210 ◽  
Author(s):  
A. E. Sikora ◽  
R. Zielke ◽  
K. Datta ◽  
J. R. Maddock
Keyword(s):  
Cell Elongation ◽  
Essential Role ◽  
Vibrio Harveyi ◽  
Ribosomal Subunit ◽  
The Other ◽  
Ribosomal Particle

ABSTRACT It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle. In light of our results, we predict that the V. harveyi CgtAV protein plays a similar essential role to that seen for Obg/CgtA proteins in other bacteria.

scholarly journals Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis

2014 ◽  
Vol 70 (7) ◽  
pp. 918-921 ◽  
Author(s):  
Sanduo Zheng ◽  
Keqiong Ye
Keyword(s):  
Diffraction Analysis ◽  
Associated Factors ◽  
Ribosome Biogenesis ◽  
X Ray Diffraction ◽  
Unit Cell Parameters ◽  
Vast Number ◽  
X Ray ◽  
Cell Parameters ◽  
Early Processing ◽  
Ribosomal Particle

Eukaryotic ribosome synthesis requires a vast number of transiently associated factors. Mpp10, Imp3 and Imp4 form a protein complex in the 90S pre-ribosomal particle that conducts early processing of 18S rRNA. Here, a short fragment of Mpp10 was identified to associate with and increase the solubility of Imp3. An Imp3–Mpp10 complex was co-expressed, co-purified and co-crystallized. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 2.1 Å resolution and belonged to space groupP212121, with unit-cell parametersa= 51.6,b= 86.9,c= 88.7 Å.

Physical studies on the ribosomal "A" protein from two archaebacteria, Halobacterium cutirubrum and Methanobacterium thermoautotrophicum

1984 ◽  
Vol 62 (1) ◽  
pp. 44-48 ◽  
Author(s):  
A. T. Gudkov ◽  
S. Yu Venyaminov ◽  
A. T. Matheson
Keyword(s):  
Escherichia Coli ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Methanobacterium Thermoautotrophicum ◽  
Sedimentation Equilibrium ◽  
Effect Of Temperature ◽  
Extreme Halophile ◽  
Moderate Halophile ◽  
Circular Dichroïsm

Physical studies on the effect of temperature and ionic conditions on the secondary, tertiary, and quaternary structure of the ribosomal "A" protein, equivalent to L7/L12 in Escherichia coli, from two archaebacteria were performed using circular dichroism and sedimentation equilibrium measurements. The two archaebacteria investigated were Halobacterium cutirubrum, an extreme halophile, and Methanobacterium thermoautotrophicum, a thermophile which also showed properties of a moderate halophile. The changes in the secondary structure and the thermostability of these proteins were directly related to the internal salt concentrations of the two archaebacteria. At the higher salt concentrations the changes in the secondary structure resulted in changes in the tertiary and quaternary structure of these proteins.

scholarly journals Aminoacylation of an unusual tRNACys from an extreme halophile

RNA ◽  
2003 ◽  
Vol 9 (7) ◽  
pp. 794-801 ◽  
Author(s):  
C. EVILIA
Keyword(s):  
Extreme Halophile

The state of binding of intracellular K+ in Halobacterium cutirubrum

1972 ◽  
Vol 18 (7) ◽  
pp. 993-995 ◽  
Author(s):  
Janos K. Lanyi ◽  
Melvin P. Silverman
Keyword(s):  
Atomic Absorption ◽  
Concentration Gradient ◽  
Equilibrium Dialysis ◽  
The State ◽  
Extreme Halophile ◽  
Dialysis Experiment

Intracellular K+ in lysed freeze-thawed cell pastes of the extreme halophile, H. cutirubrum, diffused readily against a concentration gradient during equilibrium dialysis, whereas the intracellular Mg2+ was not readily diffusible. The K+ activity in the lysed cell pastes, determined directly by K+ ion-specific electrode, was comparable to the total K-content of the cells, as determined by atomic absorption. This finding and the results of the dialysis experiment suggest that the intracellular K+ in H. cutirubrum is not bound.

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