Number and molecular weights of the basic proteins of rat liver ribosomes

1978 ◽  
Vol 163 (1) ◽  
pp. 101-112 ◽  
Author(s):  
H. Welfle ◽  
M. Goerl ◽  
H. Bielka
Keyword(s):  
Rat Liver ◽  
Basic Proteins ◽  
Molecular Weights ◽  
Liver Ribosomes

A Comparison of Plasminogen Activators Derived from Rat Plasma, Primary Rat Hepatocytes and Isolated Perfused Rat Liver

1982 ◽  
Vol 47 (02) ◽  
pp. 166-172 ◽  
Author(s):  
Yoav Sharoni ◽  
Maria C Topal ◽  
Patricia R Tuttle ◽  
Henry Berger
Keyword(s):  
Rat Liver ◽  
Isoelectric Point ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Plasminogen Activators ◽  
Rat Plasma ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Molecular Weights ◽  
Physiological Relevance

SummaryOf the two cell types it was possible to culture from the dissociated rat liver, hepatocytes and Kupffer cells, only the former were fibrinolytically active. Rat hepatocytes during the first 24 hr in culture secreted two plasminogen activators with molecular weights identical to those found in rat plasma, an 80,000-dalton form (PA-80) and a 45,000-dalton form (PA-45). Partially purified preparations of plasminogen activators from both sources were subjected to isoelectric focusing (IEF) to compare characteristics further. There were three distinct peaks of PA-45 in each preparation with isoelectric points of 7.1, 7.2 and 7.4; all electrophoretic forms had the same low affinity to fibrin. PA-80 from both sources displayed similar IEF profiles with forms ranging from pH values of 7 to 8, all with the same high affinity to fibrin. The major form of PA-80 in the plasma preparation had an isoelectric point of 7.9 whereas that in the hepatocyte preparation had an isoelectric point of 7.6. The isolated perfused rat liver was also shown to produce both PA-80 and PA-45 emphasizing the physiological relevance of the findings with hepatocytes. It is concluded that in the rat hepatocytes contribute to the plasma profile with regard to the plasminogen activator content.

Characterization and Partial Purification of Heterodisperse Polysomal RNAs in Normal and Neoplastic Cells

1972 ◽  
Vol 58 (2) ◽  
pp. 71-94
Author(s):  
Ada Sacchi ◽  
Gianni Chinali ◽  
Susetta Pons ◽  
Michela Galdieri ◽  
Piero Cammarano
Keyword(s):  
Size Distribution ◽  
Density Gradient ◽  
Rat Liver ◽  
Sucrose Density Gradient ◽  
Partial Purification ◽  
Messenger Rnas ◽  
Alkaline Ph ◽  
Sedimentation Profile ◽  
Molecular Weights ◽  
Phenol Extraction

The size distribution of cytoplasmic messenger RNAs (m-RNA) has been studied in rat liver and in monodifferentiated cells (mouse reticulocytes and myelomas). It has been found that the RNA which exhibits a « rapid turnover » and a polydisperse profile of radioactivity is refractory to phenol extraction. This property has been exploited to selectively isolate m–RNA from the phenol residue by means of an extraction at an alkaline pH. The sucrose density gradient profiles of m–RNA isolated from monodifferentiated cells show monodisperse peaks having the sedimentation coefficients expected on the basis of the molecular weights of monocistronic messages for α and β chains of hemoglobin (reticulocytes) and L and H chains of immunoglobulin (myelomas). The sedimentation profile of cytoplasmic m–RNA associated with rat liver polysomes shows a much broader distribution, with sedimentation coefficients ranging from 8 S to 28 S.

scholarly journals Binding of Purified Antialbumin by Rat Liver Ribosomes

1965 ◽  
Vol 240 (7) ◽  
pp. 3009-3015 ◽  
Author(s):  
William A. Warren ◽  
Theodore Peters
Keyword(s):  
Rat Liver ◽  
Liver Ribosomes

Identification of proteins at the peptidyl-tRNA binding site of rat liver ribosomes

1981 ◽  
Vol 184 (3) ◽  
pp. 551-556 ◽  
Author(s):  
Steven Fabijanski ◽  
Maria Pellegrini
Keyword(s):  
Binding Site ◽  
Rat Liver ◽  
Trna Binding ◽  
Liver Ribosomes

scholarly journals Biosynthesis of Plasma Lipoproteins by Rat Liver Ribosomes

1968 ◽  
Vol 243 (1) ◽  
pp. 192-199 ◽  
Author(s):  
Jacqueline J. Bungenberg de Jong ◽  
Julian B. Marsh
Keyword(s):  
Rat Liver ◽  
Plasma Lipoproteins ◽  
Liver Ribosomes

5S Ribonucleic acid-protein complex extracted from rat liver ribosomes by formamide

Biochemistry ◽  
1972 ◽  
Vol 11 (12) ◽  
pp. 2323-2326 ◽  
Author(s):  
Mary L. Petermann ◽  
Mary G. Hamilton ◽  
Amalia Pavlovec
Keyword(s):  
Rat Liver ◽  
Protein Complex ◽  
Ribonucleic Acid ◽  
Acid Protein ◽  
Liver Ribosomes

scholarly journals ATPase and GTPase Activities Isolated from Rat Liver Ribosomes

1974 ◽  
Vol 43 (2) ◽  
pp. 343-348 ◽  
Author(s):  
Friedrich Grummt ◽  
Ingrid Grummt ◽  
Volker A. Erdmann
Keyword(s):  
Rat Liver ◽  
Liver Ribosomes
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