In vitro transcription of the ribosomal RNA genes of E. coli DNA

1977 ◽  
Vol 151 (3) ◽  
pp. 305-312 ◽  
Author(s):  
János Sümegi ◽  
Andor Udvardy ◽  
Pál Venetianer
Keyword(s):  
Ribosomal Rna ◽  
In Vitro Transcription ◽  
Ribosomal Rna Genes ◽  
E Coli ◽  
Rna Genes

In vitro Transcription of Escherichia coli Ribosomal RNA Genes

Nature ◽  
1972 ◽  
Vol 235 (5337) ◽  
pp. 329-333 ◽  
Author(s):  
WILLIAM A. HASELTINE
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
In Vitro Transcription ◽  
Ribosomal Rna Genes ◽  
Rna Genes

scholarly journals Transcription initiation of Xenopus 5S ribosomal RNA genes in vitro

1979 ◽  
Vol 7 (4) ◽  
pp. 947-958 ◽  
Author(s):  
Laurence J. Korn ◽  
Edward H. Birkenmeier ◽  
Donald D. Brown
Keyword(s):  
Ribosomal Rna ◽  
Transcription Initiation ◽  
Ribosomal Rna Genes ◽  
5S Ribosomal Rna ◽  
Rna Genes

In vitro transcription of three adjacent E. coli transfer RNA genes

Nature ◽  
1974 ◽  
Vol 250 (5464) ◽  
pp. 320-323 ◽  
Author(s):  
JACOB I. GRIMBERG ◽  
VIOLET DANIEL
Keyword(s):  
In Vitro Transcription ◽  
Transfer Rna ◽  
E Coli ◽  
Rna Genes

Study of the function of Escherichia coli ribosomal RNA through site-directed mutagenesis

1990 ◽  
Vol 68 (1) ◽  
pp. 169-179 ◽  
Author(s):  
Daniel Leclerc ◽  
Léa Brakier-Gingras
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Ribosomal Proteins ◽  
Large Body ◽  
Ribosomal Rna Genes ◽  
16S Ribosomal Rna ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Rna Genes

Various approaches have been developed to study how mutations in Escherichia coli ribosomal RNA affect the function of the ribosome. Most of them are in vivo approaches, where mutations are introduced in a specialized plasmid harboring the ribosomal RNA genes. The mutated plasmids are then expressed in an appropriate host, where they can confer resistance to antibiotics whose target is the ribosome. Conditions can be used where the host ribosomal RNA genes or the host ribosomes are selectively inactivated, and the effect of the mutations on ribosome assembly and function can be studied. Another approach, which has been developed mainly with 16S ribosomal RNA, can be used entirely in vitro. In this approach, a plasmid has been constructed which contains the 16S ribosomal RNA gene under control of a T7 promoter. Mutations can be introduced in the 16S ribosomal RNA sequence and the mutated 16S ribosomal RNAs are produced by in vitro transcription. It is then possible to investigate how the mutations affect the assembly of the 16S ribosomal RNA into 30S subunits and the activity of the reconstituted 30S subunits in cell-free protein synthesis assays. Although these approaches are recent, they have already provided a large body of interesting information, relating specific RNA sequences to interactions with ribosomal proteins, to ribosome function, and to its response to antibiotics.Key words: ribosomal RNA, ribosome, site-directed mutagenesis, antibiotic resistance.

scholarly journals Effect of lucanthone (miracil D) on transcription of ribosomal RNA genes from Tetrahymena in vivo and in vitro

1979 ◽  
Vol 6 (7) ◽  
pp. 2391-2402 ◽  
Author(s):  
Ole Westergaard ◽  
Elmar Gocke ◽  
Ole F. Nielsen ◽  
Johan C. Leer
Keyword(s):  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Rna Genes

Activation of ribosomal RNA genes in pre-implantation In Vitro-produced and nuclear transfer bovine embryos

2000 ◽  
Vol 35 (6) ◽  
pp. 255-259 ◽  
Author(s):  
J Laurincik ◽  
V Zakhartchenko ◽  
B Avery ◽  
M Stojkovic ◽  
G Brem ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Nuclear Transfer ◽  
Ribosomal Rna Genes ◽  
Bovine Embryos ◽  
Rna Genes

Activation of ribosomal RNA genes in porcine embryos produced in vitro or by somatic cell nuclear transfer

2006 ◽  
Vol 74 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Bolette Bjerregaard ◽  
Hanne G. Pedersen ◽  
Anne S. Jakobsen ◽  
Lee F. Rickords ◽  
Liangxue Lai ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Ribosomal Rna ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Ribosomal Rna Genes ◽  
Rna Genes
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