The genes for the eight subunits of the membrane bound ATP synthase of Escherichia coli

1981 ◽  
Vol 183 (3) ◽  
pp. 463-472 ◽  
Author(s):  
Flemming G. Hansen ◽  
Jørgen Nielsen ◽  
Erik Riise ◽  
Kaspar von Meyenburg
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Membrane Bound

scholarly journals Physiological and morphological effects of overproduction of membrane-bound ATP synthase in Escherichia coli K-12.

1984 ◽  
Vol 3 (8) ◽  
pp. 1791-1797 ◽  
Author(s):  
K. von Meyenburg ◽  
B.B. Jørgensen ◽  
B. van Deurs
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Membrane Bound ◽  
K 12

The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit δ of the membrane bound ATP synthase of Escherichia coli

1981 ◽  
Vol 184 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Jørgen Nielsen ◽  
Flemming G. Hansen ◽  
Jürgen Hoppe ◽  
Peter Friedl ◽  
Kaspar von Meyenburg
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Atp Synthase ◽  
Membrane Bound

Proton conduction by subunit a of the membrane-bound ATP synthase of Escherichia coli revealed after induced overproduction.

1985 ◽  
Vol 4 (9) ◽  
pp. 2357-2363 ◽  
Author(s):  
K. von Meyenburg ◽  
B.B. Jørgensen ◽  
O. Michelsen ◽  
L. Sørensen ◽  
J.E. McCarthy
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Proton Conduction ◽  
Subunit A ◽  
Membrane Bound

Promoters of the atp operon coding for the membrane-bound ATP synthase of Escherichia coli Mapped by Tn 10 insertion mutations

1982 ◽  
Vol 188 (2) ◽  
pp. 240-248 ◽  
Author(s):  
Kaspar von Meyenburg ◽  
Birgitte Bojer Jørgensen ◽  
Jørgen Nielsen ◽  
Flemming G. Hansen
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Membrane Bound ◽  
Insertion Mutations

scholarly journals Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jasmine M. Hershewe ◽  
Katherine F. Warfel ◽  
Shaelyn M. Iyer ◽  
Justin A. Peruzzi ◽  
Claretta J. Sullivan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Synthetic Biology ◽  
Membrane Protein ◽  
Membrane Vesicles ◽  
Processing Methods ◽  
Glycoprotein Synthesis ◽  
On Demand ◽  
Free Gene ◽  
Membrane Bound

AbstractCell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been limited. Here, we address this limitation by characterizing native membrane vesicles in Escherichia coli-based CFE extracts and describing methods to enrich vesicles with heterologous, membrane-bound machinery. As a model, we focus on bacterial glycoengineering. We first use multiple, orthogonal techniques to characterize vesicles and show how extract processing methods can be used to increase concentrations of membrane vesicles in CFE systems. Then, we show that extracts enriched in vesicle number also display enhanced concentrations of heterologous membrane protein cargo. Finally, we apply our methods to enrich membrane-bound oligosaccharyltransferases and lipid-linked oligosaccharides for improving cell-free N-linked and O-linked glycoprotein synthesis. We anticipate that these methods will facilitate on-demand glycoprotein production and enable new CFE systems with membrane-associated activities.

Sign in / Sign up

Export Citation Format

Share Document