Bridging large bone defects with a xenograft composited with autologous bone marrow

1982 ◽  
Vol 6 (2) ◽  
pp. 79-85 ◽  
Author(s):  
D. Gupta ◽  
S. Khanna ◽  
S. M. Tuli
Keyword(s):  
Bone Marrow ◽  
Autologous Bone Marrow ◽  
Bone Defects ◽  
Autologous Bone ◽  
Large Bone ◽  
Large Bone Defects

Repair of Large Bone Defects with the Use of Autologous Bone Marrow Stromal Cells

2001 ◽  
Vol 344 (5) ◽  
pp. 385-386 ◽  
Author(s):  
Rodolfo Quarto ◽  
Maddalena Mastrogiacomo ◽  
Ranieri Cancedda ◽  
Sergei M. Kutepov ◽  
Vladimir Mukhachev ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Autologous Bone Marrow ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Autologous Bone ◽  
Large Bone ◽  
Large Bone Defects

scholarly journals Restoration of Critical-Sized Defects in the Rabbit Mandible Using Autologous Bone Marrow Stromal Cells Hybridized with Nano-β-tricalcium Phosphate/Collagen Scaffolds

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Xuehui Zhang ◽  
Mingming Xu ◽  
Xinggang Liu ◽  
Feng Zhang ◽  
Yan Wei ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Tricalcium Phosphate ◽  
Bone Marrow Stromal Cells ◽  
Autologous Bone Marrow ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Collagen Scaffolds ◽  
Autologous Bone

Nano-β-tricalcium phosphate/collagen (n-β-TCP/Col) is considered with good osteoconductivity. However, the therapeutic effectiveness of n-β-TCP/Col scaffolds in combination with autologous bone marrow stromal cells (BMSCs) remains to be elucidated for the repair of critical-sized bone defects. In this study, we found that n-β-TCP/Col scaffolds exhibited high biocompatibilityin vitro. The introduction of BMSCs expandedin vitroto the scaffolds dramatically enhanced their efficiency to restore critical-sized bone defects, especially during the initial stage after implantation. Collectively, these results suggest that autologous BMSCs in n-β-TCP/Col scaffolds have the potential to be applied in bone tissue engineering.

Cell-Based Therapy by Implanted Human Bone Marrow-Derived Mononuclear Cells Improved Bone Healing of Large Bone Defects in Rats

2015 ◽  
Vol 21 (9-10) ◽  
pp. 1565-1578 ◽  
Author(s):  
Caroline Seebach ◽  
Dirk Henrich ◽  
Alexander Schaible ◽  
Borna Relja ◽  
Manfred Jugold ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Healing ◽  
Mononuclear Cells ◽  
Bone Defects ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Based Therapy ◽  
Large Bone ◽  
Large Bone Defects

scholarly journals Repair of orbital bone defects in canines using grafts of enriched autologous bone marrow stromal cells

2014 ◽  
Vol 12 (1) ◽  
pp. 123 ◽  
Author(s):  
Yefei Wang ◽  
Xiaoping Bi ◽  
Huifang Zhou ◽  
Yuan Deng ◽  
Jing Sun ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Autologous Bone Marrow ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Autologous Bone

Regeneration of large bone defects in sheep using bone marrow stromal cells

2008 ◽  
Vol 2 (5) ◽  
pp. 253-262 ◽  
Author(s):  
P. Giannoni ◽  
M. Mastrogiacomo ◽  
M. Alini ◽  
S. G. Pearce ◽  
A. Corsi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Large Bone ◽  
Large Bone Defects

The Application of a New Composite in Large Bone Defects using Fibrillar Collagen and HA/TCP

1987 ◽  
Vol 110 ◽  
Author(s):  
Masaaki Uratsuji ◽  
T. W. Bauer ◽  
S. I. Reger
Keyword(s):  
Bone Marrow ◽  
Biological Response ◽  
Volume Ratio ◽  
Bone Defects ◽  
Autogenous Bone ◽  
Fibrillar Collagen ◽  
Group Iii ◽  
Group I ◽  
Large Bone ◽  
Large Bone Defects

The handling property and short term biological response to a new composite of fibrillar collagen (FC) and porous calcium phosphate (HA/TCP) were studied. A 4 mm by 20 mm defect was created in the femora of rabbits. The rabbits were divided into four treatment groups and sacrificed from each group at 4, 8 and 12 weeks. In each group, the defect was treated as follows: in Group I with autogenous bone marrow; in Group II with FC and HA/TCP; and in Group III with FC and HA/TCP with bone marrow in volume ratio of 3:1. In the fourth group, the defect was unfilled. The femora were excised and studied by microradiography or histology or both. The FC could improve the handling property of the HA/TCP granules. Although the composite of FC and HA/TCP was not osteoinductive, the defects in Groups II and III showed good healing at 12 weeks without signs of inflammation. The results showed the composite of FC and HA/TCP to be an effective filler for large bone defects especially when mixed with autogenous bone marrow.

scholarly journals Evaluation of Human Bone Marrow Mesenchymal Stromal Cell (MSC) Functions on a Biomorphic Rattan-Wood-Derived Scaffold: A Comparison between Cultured and Uncultured MSCs

2021 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Payal Ganguly ◽  
Jehan J. El-Jawhari ◽  
James Vun ◽  
Peter V. Giannoudis ◽  
Elena A. Jones
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Endothelial Cells ◽  
Bone Defects ◽  
Human Endothelial Cells ◽  
Transcriptional Signature ◽  
Biological Potency ◽  
Large Bone ◽  
Vegf Release ◽  
Large Bone Defects

The reconstruction of large bone defects requires the use of biocompatible osteoconductive scaffolds. These scaffolds are often loaded with the patient’s own bone marrow (BM) cells to facilitate osteoinductivity and biological potency. Scaffolds that are naturally sourced and fabricated through biomorphic transitions of rattan wood (B-HA scaffolds) offer a unique advantage of higher mechanical strength and bioactivity. In this study, we investigated the ability of a biomorphic B-HA scaffold (B-HA) to support the attachment, survival and gene expression profile of human uncultured BM-derived mesenchymal stromal cells (BMSCs, n = 6) and culture expanded MSCs (cMSCs, n = 7) in comparison to a sintered, porous HA scaffold (S-HA). B-HA scaffolds supported BMSC attachment (average 98%) and their survival up to 4 weeks in culture. Flow cytometry confirmed the phenotype of cMSCs on the scaffolds. Gene expression indicated clear segregation between cMSCs and BMSCs with MSC osteogenesis- and adipogenesis-related genes including RUNX2, PPARγ, ALP and FABP4 being higher expressed in BMSCs. These data indicated a unique transcriptional signature of BMSCs that was distinct from that of cMSCs regardless of the type of scaffold or time in culture. There was no statistical difference in the expression of osteogenic genes in BMSCs or cMSCs in B-HA compared to S-HA. VEGF release from cMSCs co-cultured with human endothelial cells (n = 4) on B-HA scaffolds suggested significantly higher supernatant concentration with endothelial cells on day 14. This indicated a potential mechanism for providing vasculature to the repair area when such scaffolds are used for treating large bone defects.

scholarly journals Кісткова репарація щелеп під впливом суміші біоактивного скла і аутологічного кісткового мозку

2018 ◽  
Author(s):  
Ya. V. Pyuryk ◽  
Hr. V. Pohoretska ◽  
L. O. Patskan
Keyword(s):  
Bone Marrow ◽  
Clinical Practice ◽  
Bioactive Glass ◽  
Blood Clot ◽  
Maxillofacial Surgery ◽  
Autologous Bone Marrow ◽  
Bone Defects ◽  
Odontogenic Cysts ◽  
Jaw Cysts ◽  
Autologous Bone

Summary. Operation on removing odontogenic jaw cysts is one of the most common surgical interventions. Patients with radicular cysts of the maxillary bones make up about 6 % in the overall structure of dental morbidity. After the removal of cysts the bone cavities are formed, the healing of which is accompanied by frequent suppuration and is long-lasting. To speed up the healing of bone cavity using various grafts made of native or synthetic bone.The aim of the study – to improve the efficacy of treatment for odontogenic cysts, we decided to combine autologous bone marrow with an artificial bone substitute, creating a mixture that, by replacing bone defects with it, would optimize reparative processes and thus restore the anatomical and functional properties of the dento-facial system.Materials and Methods. 60 patients aged 18–65 years who were operated on the basis of radicular jaw cysts were examined. The patients were on inpatient treatment at the Department of Maxillofacial Surgery of Ivano-Frankivsk Regional Clinical Hospital. The diagnosis of the disease was verified on the basis of the data of the X-ray examination. The radiographic dimensions of the cysts were within 1.5–3.5 cm. Depending on the type of material used to substitute postoperative bone cavities, patients were divided into III groups: I – in 20 patients restoration was made a blood clot; II – in 20 patients postoperative bone defects were replaced with bioactive glass, and ІІІ – in 20 patients postoperative bone defects were replaced with a mixture of autologous bone marrow and bioactive glass.Conclusions. The developed composite material of bioactive glass with the addition of an autologous bone marrow is an inductor and a source of osteogenesis, which allows to recommend it as a method of choice in the treatment of patients with bone defects of the jaws that are formed after the removal of odontogenic cysts. Thus, the prospectivity and importance of the use of bioplastic material in the clinical practice of marrow bioactive mixture in clinical practice will significantly expand the possibilities of modern reconstructive and restorative maxillofacial surgery.

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