Complementation analysis of auxotrophic mutants of the moss, Physcomitrella patens, using protoplast fusion

1977 ◽  
Vol 155 (1) ◽  
pp. 103-107 ◽  
Author(s):  
N. H. Grimsley ◽  
N. W. Ashton ◽  
D. J. Cove
Keyword(s):  
Protoplast Fusion ◽  
Physcomitrella Patens ◽  
Complementation Analysis ◽  
Auxotrophic Mutants

Somatic Hybridization in the Moss Physcomitrella patens Using PEG-Induced Protoplast Fusion

2009 ◽  
Vol 2009 (2) ◽  
pp. pdb.prot5141-pdb.prot5141 ◽  
Author(s):  
D. J. Cove ◽  
P.-F. Perroud ◽  
A. J. Charron ◽  
S. F. McDaniel ◽  
A. Khandelwal ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Physcomitrella Patens ◽  
Somatic Hybridization

Segregation of altered parental properties in fusions between Saccharomyces cerevisiae and the D-xylose fermenting yeasts Candida shehatae and Pichia stipitis

1992 ◽  
Vol 38 (12) ◽  
pp. 1233-1237 ◽  
Author(s):  
Abindra S. Gupthar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Polyethylene Glycol ◽  
Gene Transfer ◽  
Key Words ◽  
Protoplast Fusion ◽  
Ethanol Tolerance ◽  
Pichia Stipitis ◽  
Candida Shehatae ◽  
Auxotrophic Mutants ◽  
Parental Type

A prototrophic strain of Saccharomyces cerevisiae CSIR Y190 MATa xyl−, resistant to high levels of ethanol, was hybridized with xylose-fermenting, auxotrophic mutants of Candida shehatae and Pichia stipitis through polyethylene glycol-induced protoplast fusion in an attempt to produce ethanol-tolerant, xylose-fermenting hybrids. Mononucleate fusants were obtained, but these dissociated into a mixture of parental-type segregants. Purified Candida- and Pichia-resembling segregants failed to acquire improved ethanol tolerance but expressed other novel properties of S. cerevisiae, suggesting that karyogamy was impaired after internuclear gene transfer. Key words: Pichia, Candida, Saccharomyces protoplast fusion.

Candida fennica: enhancement of protoplast formation and fusion

1992 ◽  
Vol 38 (8) ◽  
pp. 807-810 ◽  
Author(s):  
Carolina M. M. Andrade ◽  
Flavia M. Oliveira ◽  
Valter R. Linardi
Keyword(s):  
Key Words ◽  
Protoplast Fusion ◽  
Ultraviolet Light ◽  
Protoplast Regeneration ◽  
Protoplast Formation ◽  
Regeneration Frequency ◽  
Fusion Frequency ◽  
Auxotrophic Mutants ◽  
Enrichment Procedure ◽  
Dna Contents

Auxotrophic mutants were obtained by exposing Candida fennica FTPT/CCT-8903 to ultraviolet light and treating the cells with nystatin in an enrichment procedure. The mutants were used for protoplast fusion. The highest protoplast regeneration frequency was obtained when cells were treated with 10 mg NovoZym 234/mL in the presence of 0.8 M KCl for 60 min. Fusion frequency was in the range 5.84 × 10−5 to 7.2 × 10−5. Five prototrophic hybrid colonies, designated S9, S15, S17, S18, and S25, were isolated, and their DNA contents were measured. These measurements indicated that the S17 hybrid is haploid, S9 and S18 are polyploid, and S15 and S25 are both aneuploid. Key words: Candida fennica, nystatin, auxotrophic mutants, protoplast fusion.

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