The role of mitochondria in carbon catabolite repression in yeast

1976 ◽  
Vol 148 (2) ◽  
pp. 205-211 ◽  
Author(s):  
P. Haußmann ◽  
F. K. Zimmermann
Keyword(s):  
Catabolite Repression ◽  
Carbon Catabolite Repression

scholarly journals Carbon catabolite repression in pectin digestion by phytopathogen Dickeya dadantii

2021 ◽  
Author(s):  
Shiny Martis B ◽  
Michel Droux ◽  
William Nasser ◽  
Sylvie Reverchon ◽  
Sam Meyer
Keyword(s):  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Degradation Pathway ◽  
Hplc Method ◽  
Metabolic Switch ◽  
Dickeya Dadantii ◽  
Transcriptional Regulatory ◽  
Genes Encoding ◽  
Regulatory Model

The catabolism of pectin from the plant cell walls plays a crucial role in the virulence of the phytopathogen Dickeya dadantii. In particular, the timely expression of pel genes encoding major pectate lyases is essential to circumvent the plant defense systems and induce a massive pectinolytic activity during the maceration phase. While previous studies identified the role of a positive feedback loop specific to the pectin degradation pathway, here we show that the pel> expression pattern is controlled by a metabolic switch between glucose and pectin. We develop a dynamical and quantitative regulatory model of this process integrating the two main regulators CRP and KdgR related to these two sources of carbon, and reproducing the concentration profiles of the associated metabolites, cAMP and KDG respectively, quantified using a new HPLC method. The model involves only 5 adjustable parameters, and recapitulates the dynamics of these metabolic pathways during bacterial growth together with the regulatory events occurring at the promoters of two major pel genes, pelE and pelD. It highlights their activity as an instance of carbon catabolite repression occurring at the transcriptional regulatory level, and directly related to the virulence of D. dadantii. The model also shows that quantitative differences in the binding properties of common regulators at these two promoters resulted in a qualitative different role of pelD and pelE in the metabolic switch, and also likely in conditions of infection, explaining their evolutionary conservation as separate genes in this species.

scholarly journals Transformation of Escherichia coli with the pGLO Plasmid: Going beyond the Kit

2019 ◽  
Vol 81 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Charles E. Deutch
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Transformation Process ◽  
Host Restriction ◽  
Green Fluorescent ◽  
Restriction Modification ◽  
Restriction Modification Systems

The Bio-Rad pGLO bacterial transformation kit is commonly used to demonstrate this form of genetic exchange, which occurs in bacteria and eukaryotes and which differs fundamentally from transduction and conjugation. The basic experiment leads to the formation of green fluorescent colonies of Escherichia coli and can be extended to illustrate the specificity of the interaction between sugars and the AraC protein, the phenomenon of carbon catabolite repression, the substrate specificity of the β-lactamase encoded by the plasmid, and the role of host restriction/modification systems in the transformation process. pGLO DNA also can be isolated using plasmid mini-prep kits, analyzed with restriction endonucleases, and used to study the conditions for transformation in more detail.

Defining the genome-wide role of CRE1 during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis

2014 ◽  
Vol 73 ◽  
pp. 93-103 ◽  
Author(s):  
Amanda Cristina Campos Antoniêto ◽  
Lílian dos Santos Castro ◽  
Rafael Silva-Rocha ◽  
Gabriela Felix Persinoti ◽  
Roberto Nascimento Silva
Keyword(s):  
Trichoderma Reesei ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Rna Seq ◽  
Genome Wide

scholarly journals Role of the cAMP-Dependent Carbon Catabolite Repression in Capsular Polysaccharide Biosynthesis in Klebsiella pneumoniae

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e54430 ◽  
Author(s):  
Ching-Ting Lin ◽  
Yu-Ching Chen ◽  
Tzyy-Rong Jinn ◽  
Chien-Chen Wu ◽  
Yi-Ming Hong ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Catabolite Repression ◽  
Capsular Polysaccharide ◽  
Carbon Catabolite Repression ◽  
Polysaccharide Biosynthesis

scholarly journals Intracellular 2-keto-3-deoxy-6-phosphogluconate is the signal for carbon catabolite repression of phenylacetic acid metabolism in Pseudomonas putida KT2440

Microbiology ◽  
2009 ◽  
Vol 155 (7) ◽  
pp. 2420-2428 ◽  
Author(s):  
Juhyun Kim ◽  
Jinki Yeom ◽  
Che Ok Jeon ◽  
Woojun Park
Keyword(s):  
Pseudomonas Putida ◽  
Transcriptional Repression ◽  
Catabolite Repression ◽  
Phenylacetic Acid ◽  
Carbon Catabolite Repression ◽  
Transcriptional Level ◽  
Pseudomonas Putida Kt2440 ◽  
Coa Ligase ◽  
Additional Support

The growth pattern of Pseudomonas putida KT2440 in the presence of glucose and phenylacetic acid (PAA), where the sugar is used in preference to the aromatic compound, suggests that there is carbon catabolite repression (CCR) of PAA metabolism by glucose or gluconate. Furthermore, CCR is regulated at the transcriptional level. However, this CCR phenomenon does not occur in PAA-amended minimal medium containing fructose, pyruvate or succinate. We previously identified 2-keto-3-deoxy-6-phosphogluconate (KDPG) as an inducer of glucose metabolism, and this has led to this investigation into the role of KDPG as a signal compound for CCR. Two mutant strains, the edd mutant (non-KDPG producer) and the eda mutant (KDPG overproducer), grew in the presence of PAA but not in the presence of glucose. The edd mutant utilized PAA even in the presence of glucose, indicating that CCR had been abolished. This observation has additional support from the finding that there is high phenylacetyl-CoA ligase activity in the edd mutant, even in the presence of glucose+PAA, but not in wild-type cells under the same conditions. Unlike the edd mutant, the eda mutant did not grow in the presence of glucose+PAA. Interestingly, there was no uptake and/or metabolism of PAA in the eda mutant cells under the same conditions. Targeted disruption of PaaX, a repressor of the PAA operon, had no effect on CCR of PAA metabolism in the presence of glucose, suggesting that there is another transcriptional repression system associated with the KDPG signal. This is the first study to demonstrate that KDPG is the true CCR signal of PAA metabolism in P. putida KT2440.

Carbon catabolite repression gene creA regulates morphology, aflatoxin biosynthesis and virulence in Aspergillus flavus

2018 ◽  
Vol 115 ◽  
pp. 41-51 ◽  
Author(s):  
Opemipo Esther Fasoyin ◽  
Bin Wang ◽  
Mengguang Qiu ◽  
Xiaoyun Han ◽  
Kuang-Ren Chung ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Aflatoxin Biosynthesis

Carbon catabolite repression occurrence in photo fermentation of ethanol-rich substrates

2021 ◽  
Vol 297 ◽  
pp. 113371
Author(s):  
Grazia Policastro ◽  
Marco Giugliano ◽  
Vincenzo Luongo ◽  
Raffaele Napolitano ◽  
Massimiliano Fabbricino
Keyword(s):  
Catabolite Repression ◽  
Carbon Catabolite Repression
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