Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids

1979 ◽  
Vol 174 (2) ◽  
pp. 221-224 ◽  
Author(s):  
Wolfgang Schumann
Keyword(s):  
Plasmid Vector ◽  
Direct Selection ◽  
Recombinant Plasmids ◽  
Selection Of ◽  
Selection Of Transformants

scholarly journals An Improved Cloning Vector for Construction of Gene Replacements in Listeria monocytogenes

2003 ◽  
Vol 69 (5) ◽  
pp. 3020-3023 ◽  
Author(s):  
Guojie Li ◽  
S. Kathariou
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Plasmid Vector ◽  
Gene Replacement ◽  
Direct Selection ◽  
Genetic Studies ◽  
Food Borne ◽  
Food Borne Illness ◽  
Selection For ◽  
Selection Of

ABSTRACT Listeria monocytogenes is a gram-positive, facultative intracellular bacterium implicated in severe food-borne illness (listeriosis) in humans. The construction of well-defined gene replacements in the genome of L. monocytogenes has been instrumental to several genetic studies of the virulence and other attributes of the organism. Construction of such mutations by currently available procedures, however, tends to be labor intensive, and gene replacement mutants are sometimes difficult to recover due to lack of direct selection for the construct. In this study we describe the construction and use of plasmid vector pGF-EM, which can be conjugatively transferred from Escherichia coli S17-1 to L. monocytogenes and which provides the genetic means for direct selection of gene replacements.

Direct selection of recombinant plasmids in Bacillus subtilis

Gene ◽  
1982 ◽  
Vol 20 (3) ◽  
pp. 459-469 ◽  
Author(s):  
Thomas J. Gryczan ◽  
David Dubnau
Keyword(s):  
Bacillus Subtilis ◽  
Direct Selection ◽  
Recombinant Plasmids ◽  
Selection Of

A plasmid vector allowing positive selection of recombinant plasmids in Streptococcus pneumoniae

Gene ◽  
1985 ◽  
Vol 39 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Hervé Prats ◽  
Bernard Martin ◽  
Philippe Pognonec ◽  
Anne-Catherine Burger ◽  
Jean-Pierre Claverys
Keyword(s):  
Streptococcus Pneumoniae ◽  
Positive Selection ◽  
Plasmid Vector ◽  
Recombinant Plasmids ◽  
Selection Of

scholarly journals Direct selection of recombinant plasmids with chlorate

1987 ◽  
Vol 43 (2) ◽  
pp. 201-205 ◽  
Author(s):  
Jochen Reiss ◽  
Walter Klingmüller
Keyword(s):  
Direct Selection ◽  
Recombinant Plasmids ◽  
Selection Of

A novel ColEl::Tn3 plasmid vector that allows direct selection of hybrid clones in E. coli

Gene ◽  
1980 ◽  
Vol 8 (3) ◽  
pp. 301-314 ◽  
Author(s):  
Luiz Shozo Ozaki ◽  
Maeda Shuichiro ◽  
Shimada Kazunori ◽  
Takagi Yasuyuki
Keyword(s):  
Plasmid Vector ◽  
Direct Selection ◽  
E Coli ◽  
Hybrid Clones ◽  
Selection Of
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