Electron microscopy analysis of circular repetitive mitochondrial DNA molecules from genetically characterized Rho- mutants of Saccharomyces cerevisiae

1976 ◽  
Vol 146 (1) ◽  
pp. 61-78 ◽  
Author(s):  
J. Lazowska ◽  
P. P. Slonimski
Keyword(s):  
Electron Microscopy ◽  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Dna Molecules ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

scholarly journals A method for isolation of plasmid DNA replication intermediates from unsynchronized bacterial cultures for electron microscopy analysis.

1998 ◽  
Vol 45 (1) ◽  
pp. 233-240 ◽  
Author(s):  
S Srutkowska ◽  
G Konopa ◽  
G Wegrzyn
Keyword(s):  
Electron Microscopy ◽  
Dna Replication ◽  
Plasmid Dna ◽  
Microscopic Analysis ◽  
Normal Growth ◽  
Living Cells ◽  
Dna Molecules ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Replication Intermediates

Electron microscopy is a powerful technique for analysis of DNA replication intermediates. However, isolation of replicating DNA molecules from living cells is tricky and difficult, especially in the case of small DNA molecules (such as bacterial plasmids) whose initiation of replication is not easily synchronized. Here a relatively simple and rapid method for efficient isolation of replicating plasmid molecules from unsynchronized Escherichia coli cultures is described. The efficiency of this procedure is high enough for electron microscopy analysis of plasmid replication intermediates appearing in living cells in normal growth conditions. Under optimal conditions, using standard procedures of isolation of plasmid DNA, it is possible to achieve a content of only as few as 0.02 percent of replication intermediates in a plasmid DNA sample. The described method allowed us to enrich up to 100-fold the fraction of replication intermediates suitable for microscopic analysis among all plasmid molecules.

scholarly journals Electron microscopy analysis of biofilms produced by Staphylococcus aureus exposed to UV-light on the surface of SnO2 thin films

2021 ◽  
Vol 27 (S1) ◽  
pp. 3168-3170
Author(s):  
Hazel Jaynelle Morales-Rodriguez ◽  
Javier Camarillo-Cisneros ◽  
María Alejandra Favila-Pérez ◽  
Alva Rocío Castillo-González ◽  
Celia María Quiñonez-Flores ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Thin Films ◽  
Staphylococcus Aureus ◽  
Uv Light ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Sno2 Thin Films

scholarly journals Focused Ion Beam (FIB) Preparation and Electron Microscopy Analysis of Individual Microbolometer Pixels

2006 ◽  
Vol 12 (S02) ◽  
pp. 1270-1271
Author(s):  
M Olszta ◽  
J Dougherty ◽  
M Horn ◽  
EC Dickey
Keyword(s):  
Electron Microscopy ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005

scholarly journals Ileal Transposition (IT) Surgery Changing the Ultrastructure of the Transposed Segment as well as Jejunum. Histomorphometric and Electron Microscopy Analysis

2017 ◽  
Vol 28 (5) ◽  
pp. 1232-1239 ◽  
Author(s):  
Tomasz Sawczyn ◽  
Julia Zimmermann ◽  
Dominika Stygar ◽  
Michał Kukla ◽  
Katarzyna Nabrdalik ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Ileal Transposition ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis
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